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We narrowed to 980 results for: ARC

Showing: 601 - 620 of 980 results
  1. Plasmids 101: The Promoter Region – Let's Go!

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    Blog Post
    ...not a big issue for cells, but it may confound research results or even kill your cells if your gene of...right tissue-specific promoters as described by researchers at the NIH here.  Note: A. Max Juchheim contributed...Resources on Addgene.org Find Plasmids for Your Research     ...
  2. Recombinases AAV Preps

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    Collection
    ...pAAV.TBG.PI.FLPo.rBG TBG none 8 Melina Fan , Addgene Research Program Light-Inducible Recombinases ID Name Promoter...
  3. Plasmids 101: Expressing shRNAs for RNA Interference

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    Blog Post
    ...useful and most importantly, effective. In the research world, there is a myriad of tools this could be...intended target. RNAi is a valuable tool for your research arsenal, and there is still a dedicated field ...about miRNA-guided gene silencing. Nucleic Acids Research, 50(2), 617–634. https://doi.org/10.1093/nar/gkab1256...
  4. Antibodies 101: Normalization and Loading Controls for Western Blots

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    Blog Post
    ...would be directly reported by band intensity. Researchers use later steps in this chain (blue) to infer...controls (green). Created with BioRender.com. Researchers often rely on assumptions that their loading ...your particular setup. We recommend starting by searching the literature to see what is already known about...
  5. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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    Blog Post
    ... generate full libraries of mutant proteins. Researchers from the Whitehead lab developed One pot saturation...saturation mutagenesis? How would you use it in your research? References 1. Natarajan, Chandrasekhar, et al...Central PMCID: PMC3159719. 6. Firnberg, Elad, and Marc Ostermeier. “PFunkel: Efficient, Expansive, User-Defined...
  6. Antibodies 101: Single Chain Fragment Variables (scFvs)

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    Blog Post
    ...application for basic and translational research.  Basic research applications of scFvs: The SunTag system...Plasmid Collection Find antibodies for neuroscience research from the Trimmer lab Check out Addgene's latest...
  7. Filming Science Videos in the Age of Social Distancing

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    Blog Post
    ... COVID-19 pandemic. The Pandemic Pivot Back in March 2020, just four months after I started with Addgene...completely out of reach for Addgene.  So, onto research! I needed to design a system that would allow ...  Quintin Marcelino...
  8. Retrovirus Plasmids

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    Collection
    ... retroviral plasmid with your gene of interest? Search our database for retroviral transfer plasmids that...
  9. Generating Mouse Models Using CRISPR/Cas9

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    Blog Post
    ...mouse genome. With these early technologies, a researcher would first introduce a mutation into a mouse...and rushed to test it. I still remember the day, March 16, 2014, that I got my first preliminary results...CRISPR-Cas9 is an acquired immune system in bacteria and archaea. As you know well if you’ve been following the ...
  10. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

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    Blog Post
    ...efficiency of homology directed repair, with researchers hypothesizing that they can efficiently integrate...bp sequence around the desired insertion site to search for potential gRNAs. As with other homology-directed... under a Creative Commons 4.0 license. The research groups responsible for Easi-CRISPR have also recently...
  11. Hot Plasmids: Fall 2025

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    Blog Post
    ...decades since this transfer was first proposed, researchers have shown that these transplanted mitochondria...and altering the composition and dynamics. The researchers demonstrated this perturbation interrupted the...license.  In addition to chromoproteins, the researchers developed a version of the kit that uses melanin...
  12. Building Global Connections with the International Mentorship Program USA-EUROPE

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    Blog Post
    ...couldn’t be luckier with my mentor, since she does research at UK in the same field that I do: prostate cancer...highlights included remarks by the Spanish Secretary of Research, Development, and Innovation, D. Carmen Vela, ...    Rosario Fernandez-Godino is a Postdoctoral Researcher at MEEI-Harvard Medical School, where she studies...
  13. mTOR Pathway

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    Collection
    ... Cancer Research mTOR Pathway mTOR Pathway You May Also Like: Cancer...
  14. Antibodies 101: Antibody Engineering and Directed Evolution

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    Blog Post
    ...antibodies means improving research. More specific antibodies support common research tools like western blots... Antibody Topics Antibody Data Repositories and Search Engines Antibodies 101: Producing Recombinant Antibodies...
  15. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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    Blog Post
    ...high levels of spurious expression.  As such, researchers developed new tools called FLEx/DIO vectors where...expression levels over several weeks allows the researcher to determine when the optimal time to conduct...merely a new lot, be thoroughly tested in the researcher's system before beginning large scale experiments...
  16. How to Design Your gRNA for CRISPR Genome Editing

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    Blog Post
    ...CRISPR Plasmids for Your Research Find CRISPR Pooled Libraries for Your Research ... human stem cell genome editing. Nucleic Acids Research 41:9049–9061 . https://doi.org/10.1093/nar/gkt555...
  17. p53 Pathway

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    Collection
    ... Cancer Research p53 Pathway p53 Pathway You May Also Like: Cancer...
  18. Live and Let Dye: Self-Labeling Protein Tags

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    Blog Post
    ...stability, or other properties is an active area of research. If size is a concern for your application, SNAP-tag...gene of interest with these tools. Conclusion Researchers and vendors continue to develop new ligands for...Proteins Inside Living Cells. Accounts of Chemical Research, 44(9), 784–792. https://doi.org/10.1021/ar200099f...
Showing: 601 - 620 of 980 results