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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,052 results for: FER

Showing: 601 - 620 of 1052 results

  1. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    ... value, clearly communicate exactly what a site offers to their users, and most importantly, allow those...get scientists excited about what Addgene has to offer, and to help scientists find what they need - whether...wanted to highlight our blog, daily updates, and offer better exploration throughout the site.  Blugene...2011, and while you may have seen our mascot at conferences, on our website, or in social media, we wanted...

  2. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    .... The following Friday, I received the official offer. I was so happy to hear back, I accepted that day... food service industry, which followed a very different schedule. Fortunately, I was provided with a detailed...gave me enough time to become familiar with the different types of issues that were important to this function...Specifically as a co-op, it is encouraged to explore different teams and work on projects there. At this point...

  3. Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

    Type
    Blog Post
    ...plasmids for expressing different types of rhodopsins and sensors as well as references and links to other ...using both its Addgene ID number as well as the reference for its original publication. To help facilitate... citation that you can put straight into your reference section. Why do this? Not only does citing plasmids...

  4. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...the future. Studies show that the majority of job offers arise as a result of existing professional (and...interest, even excitement, in a position. Everyone prefers to hire someone that will be excited to be there...resume should also be customized to highlight different skills and to get the important information in..., you will never get the help that can make a difference. Having your resume forwarded personally gives...

  5. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...eyes to the many different jobs associated with science, especially if the conference is a big multidisciplinary...curious and interdisciplinary.   3. “There are many different ways to succeed – as loner or gregarious;  dictator...leaving academia should present their research at conferences to obtain a “crash-course in all the real life...

  6. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...nontarget strand. Protein Cas: Different CRISPR systems involve different proteins, but many of them are...laboratory applications. At Addgene, we sometimes refer to the programmable guide sequence as the spacer...scaffold. gRNA: "Guide RNA" is a general term referring to the RNAs used by Cas proteins, either a crRNA...

  7. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...the discoveries yet to come.      References and resources References Arbabi-Ghahroudi M (2017) Camelid...become appealing candidates for the detection of different cancers (Jin, et al. 2023).  So where do all these...    Figure 2: Genetically modified mice offer an alternative method to producing single-domain...

  8. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    ...general primer design, as well as primer design for different cloning applications. Primer3 and Primer3Plus...calculate how much insert and backbone you need for different types of cloning methods, such as restriction ... any cloning method. You can select from five different insert:backbone ratios. In-Fusion cloning molar...Resources on Addgene.org: Read our Molecular Biology Reference Find molecular biology protocols Browse our protocol...

  9. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    ....  3. Do you prefer being a generalist? Working on a variety of projects and in different fields of research...along a career path in these two fields looks different however. In industry the career ladder is larger...training can prepare you to present your work at conferences, help with manuscript and grant preparation, ...

  10. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type
    Blog Post
    ...compare and analyze any differences between our sequence results and the reference sequences. Using Snapgene...features To align NGS sequencing results with reference sequences To design experiments for further plasmid...Additioinal Resources on Addgene.org Molecular Biology Reference Pages Basic Molecular Biology Protocols Diagnostic...

  11. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...nomenclature throughout the rest of this blog post, GFP refers to sfGFP-GB1.   Tanenbaum et al. examined the power...SunTag? Tanenbaum et al. performed a variety of different experiments in their paper, and so can you! In...we’ll save that application for another day! References 1. Tanenbaum, Marvin E., et al. “A protein-tagging...Visit Our Fluorescent Protein Guide Page Find Luciferase Plasmids ...

  12. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...interest –  try pRS plasmid shuttle vectors. You may prefer the more traditional approach of an autonomously...strain or a mutated version of YGOI to test the different phenotypes of the specific mutations. For these...YGOI on a western blot or under a microscope, we offer a collection of vectors carrying several fusion ..., or knocking down YGOI through RNA-mediated interference (e.g. L4440) and observing the downstream effects...

  13. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...plasticware or think about conserving reagents and buffers.  However, I had very environmentally conscious...required less agar, less ethidium bromide, less stop buffer and miniscule amounts of plasmid sample.  How satisfying..., I was bothered by the fact that I was wasting buffer running my tiny gels in a normal gel box. It took... small enough to minimize the required area and buffer volume.  So, I designed my own gel box (see illustration...

  14. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...this emerging technology! Resources and References References Orguc, S., Sands, J., Sahasrabudhe, A., ...obvious next applications are to expand the tool to different brain-organ axes. Alternatively, the system could...Free Behavior. 2020 42nd Annual International Conference of the IEEE Engineering in Medicine & Biology...

  15. What the HEK?

    Type
    Blog Post
    ...Why the HEK not? References and Resources References Shaw G., et al., Preferential transformation of ...for and in HEKs. These systems built around HEKs offer an advantage to use the cell line and we will highlight...

  16. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...outside of the cell. The same protein may have different epitopes available if it is being selected out...controls in validation, as you may need to test different configurations and conditions to understand exactly... what a given protein is doing or changing in different conditions. Just remember to keep good records...when you’re building your antibody library! References and Resources More resources on Addgene’s blog...

  18. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    ...each of the gRNAs. The idea was to assign four different barcodes to each of the gRNAs and to embed these...of the recombination sites in the construct to different lengths, the density of cells that are labeled...fusions as a DNA staining dye Article contributed by Jennifer Tsang Figure 4: Double stranded DNA bound...40 mM to 100 mM NaCl but stained evenly in 1x TE buffer. The lab also used tTALE-FP in a single molecule-fluorescence...

  19. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ... together as useful and productive, forming a different constellation of interests and skills than what... experimental design and data with authors and referees. And I am constantly teaching – often explaining...Advancement of Science (AAAS), we have all sorts of different editors. Do you like science journalism? Take ...short program to build skills and connections. AAAS offers a summer program that gives graduate students an...

  20. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...calcium sensors, different iGABASnFR have been developed and are suited for different applications. The... a few versions of these jGCaMP7 sensors with different properties (for example, overall brightness or...on/off kinetics) that makes them suitable for different applications. We recently released a new variant...
Showing: 601 - 620 of 1052 results