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Showing: 601 - 620 of 858 results

  2. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...important plasmid elements – promoters, origins of replication, protein tags, and antibiotic resistance markers...proteins or co-factors, thus allowing for broad applications in a variety of experiments. LoxP (locus of ...

  4. Hot Plasmids - October 2020

    Type
    Blog Post
    ...Jerome Goudeau, Cynthia Kenyon, and colleagues at Calico Life Sciences. Like many split FPs, split-wrmScarlet...Hybrid for Multiplexed Editing and screening Applications (CHyMErA) Knockout Libraries express hybrid ...

  6. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...location of the cut and subsequent edit. In these applications, location takes priority over cutting efficiency...in double stranded cleavage and base editing applications, with high editing capacity in both contexts...

  7. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...important to consider that the same receptor can elicit different responses depending on what cell type...chemogenetics for research and potential clinical applications. Science 6436: eaav5282. https://science.sciencemag.org...

  8. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...BioRender.com In their recent ACS Synthetic Biology publication, Mary Dunlop’s lab generated a photosensitive...regions with Vivid (Vvd) photodimers. Upon the application of blue light, Vvd dimerizes bringing the C-terminal...

  9. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...experiment and they don't do anything. Viruses are complicated and they perform complex biological tasks, both...vector may undergo genomic rearrangements during replication. To avoid genomic rearrangements, try amplifying...

  10. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...rho-dependent and rho-independent. Rho factor is a helicase which assists RNA polymerase in the termination...Central PMCID: PMC104046. Wodrich H, Schambach A, Kräusslich H-G. Multiple copies of the Mason–Pfizer monkey...

  11. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...typically requires transducing with a limiting multiplicity of infection (MOI) to ensure only one copy integrates...lentiviral vector titration and in the use of multiplicity of infection (MOI) for predicting gene transfer...

  12. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...these people and bios were likely part of the publicity. Good panels do not waste time on long introductions...on 1-2 panelist for each question in a way that elicits those interesting stories and opinions. Don't let...

  14. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ..., and making sure we always maintain a backup replicates of each clone in case of contamination. We do...population of transfected clones. The technique is applicable to a wide variety of cell lines, from HEK to ...

  15. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...targeting anywhere in an exon. But things get more complicated if you’d like to use homology directed repair...editing rates. This PAM flexibility should expand applications that require very specific targeting, like homology...

  17. Pooled Library Amplifications

    Type
    Blog Post
    ...to amplify the sample for further downstream applications. As individual libraries can vary significantly...containing smaller, recombined backbones that may replicate faster. For this reason, if your library has inserts...

  18. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ... into Addgene’s CRISPR data? Read our recent publication, “The Expanding Dissemination and Distribution...support for, this and previous Addgene CRISPR publications.  References and resources References Pyhtila...

  19. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...moments. The models function as thinking tools – eliciting questions like “what does this model representt...or trying to explain your science to the general public, visit the Center for BioMolecular Modeling web...

  20. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ..., blastp, and blastx searches! Now, before you click the BLAST button, consider the Program Selection...may have noticed the “Algorithm parameters” link. Click on this link to view advanced BLAST options and ...
Showing: 601 - 620 of 858 results