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... reaction could look like this: 1 µg DNA 1 µL of each Restriction Enzyme 3 µL 10x Buffer 3 µL 10x BSA ...then add the appropriate amount of Master Mix to each tube. This will save you time and ensure consistency...

.... Wipe down all pipettes and equipment with 10% bleach prior to use. Reagent Preparation 100 mM Valproic...containing 108 mL cells: Transfer 6 mL of BCD TFX into each of two 50 mL tubes. Cap the tubes and incubate for...

...ice for 5 min. Add 150 μL of cold Solution III to each tube. Mix by inverting several times. A white precipitate...careful, the pellet is harder to see and less well attached to the tube after the 70% ethanol wash. You can...

...TikTok for bite-sized portions of antibody concepts. Each short video answers an Antibodies 101 question, ...  Rachel Leeson...

...our speed round data presentation, with two winner each receiving a $500 travel award. All participants ...  Rachel Leeson...

...the lab? Or looking for ways to supplement your teaching? You’re in luck! Addgene’s Intro to the Lab Bench...introductory science techniques. It’s also great for teachers looking to supplement their curriculum. From the...

...prime editing systems through manipulating MMR machinery. On the fifth day of CRISPR, Addgene gave to me... Rachel Leeson...

...DuanVisualizing Your Plasmid Request Data Author: Joachim Goedhart  Created in collaboration with Addgene...  Rachel Leeson...

...our tips in our new series: Addgene Lab Tips! In each short episode of this series, we’ll highlight one...that will keep the lab running like a well-oiled machine. Lab tip #1: Glove trick Putting on lab gloves ...

...'ve all got different areas of expertise, we are each equally excited to talk to you, answer any questions...  Rachel Leeson...

...good starting points to understand the contours of each topic. The video channels are also underrated and...additional resources that might be helpful, please reach out to me by email.  About the author Niko McCarty...

... can be adapted for wet and semi-dry transfer approaches. Last Update: January 24, 2022 Workflow Timeline...to load an equivalent amount of total protein for each sample. Pro-Tip The ideal total protein loaded will...

...and SITN The Most Difficult thing Vini, Amy, and Outreach Scientist Jessica Welch Have Had to Explain Importance... when Communicating with Non-Scientists How to Approach Different Audiences Listen to the SITN Podcast...

... acid sequences that got closer and closer to achieving it. Once they designed the protein computationally...drugs, all developed with their protein design approach. Congratulations to David Baker, Demis Hassabis...

... blog, please reach out to [email protected] - we'd love to hear from you!       ...  Rachel Leeson...

...everything from Falcon tube trees to giant paper mache Krampus performing routine lab maintenance tasks... Rachel Leeson...

...Therefore, in this blog post we’re taking a moment to reach out to you, our readers, and ask you to share your...different fields. Exposure - With over 100,000 views each month, the Addgene Blog is a great way to get your...

...that plasmid deposits come from 49 countries* and reached 102 countries. We started from 474 plasmid deposits...Despite COVID-19 and lab closures, we’re expecting to reach ~14,000 plasmids deposited this year once they all...

...between some much-deserved vacations, sunshine, and beach trips, of course.    *not me, though. I don't dance...                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      Rachel Leeson...

...one problem - we didn't know their name. So we reached out to you and received a plethora of options, ...  Rachel Leeson...