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We narrowed to 963 results for: tes

Showing: 601 - 620 of 963 results

  1. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...advantage of this system is that streptavidin conjugates are commercially available for a wide range of... heterogeneous cell mixes, like bulk lympho-/splenocytes, you might only be interested in one of the various...mummification). Permeabilization, as the name suggests, perforates the cell membrane to allow for antibodies to ...

  2. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...speakers talk without looking at their slides or notes. Listen to their stories and narratives (see below... that you can deliver most of your talk without notes. Don’t be hard on yourself if you stumble or make...more engaging session. The best speakers use no notes and can concentrate on delivery, rapport with the...

  3. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...them in 1885. E. coli are mainly found in the intestinal tract of animals. There are many different naturally...coli used today are descended from two individual isolates, the K-12 strain and the B strain. K-12 was isolated...typically standard; just having the gene listed indicates it is non-functional. If a gene is deleted, it...

  4. Chemogenetics Guide

    Type
    Guide
    ...luciferin) is added, the luciferase enzyme generates light that activates the opsin. The opsin may excite or ...activated through chemogenetic or optogenetic routes. Image courtesy of Ute Hochgeschwender . Table 3. Descriptions...that bind a specific ligand. Ligand binding then activates G-proteins to modulate downstream signaling. GPCRs...back-metabolized to clozapine and other clozapine metabolites and these can have off-target effects. More recent...allows for influx of Na + and/or K + ions, and activates neuronal activity. Figure 3: PSAM4-based ligand-gated...RHJ, DiBerto JF, et al. (2015). A New DREADD Facilitates the Multiplexed Chemogenetic Interrogation of...

  5. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...for the PI and lab members.  Make coffee or beer dates to talk to as many lab alumni as you can or schedule...or at least observe grant writing? Get equipment quotes? Understand administrative responsibilities? Is...this blog as a guide. Start a document to record notes and observations so you have data to review when...

  6. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ...expected fragments for that plasmid. This phenomenon indicates the presence of a multimer.   Fig. 1:... be used to detect multimers, since restriction sites in multimers usually repeat in tandem along with... of the plasmid monomer (see this article from BiteSize Bio for more information on how different plasmid...

  7. Finding Your Science Policy Path

    Type
    Blog Post
    ...Each path to science policy is unique and this creates a rich and diverse workforce. Out of all the career...interests.  Action Items:  Sign up to receive updates from ESAL and explore their Local Engagement Playbook...others (see more in Figure 2). Starting early demonstrates not only your interest to future positions, ...

  8. Antibodies 101: Validation

    Type
    Blog Post
    ...aware of any possible alternative translation start sites or alternative splicing that could lead to some ...with your protein of interest and show just the faintest band from the cells transfected with the other...work great in another context! So the next time you test an antibody (perhaps one from Addgene!), consider...

  9. CRISPR Library Amplification

    Type
    Protocol
    ... bioassay plates and remove bacteria. Use one scraper for all plates. Use two 10 mL pipettes (one for ...Antibiotic 245 mm bioassay plates (Molecular Devices, X602) Pro-Tip Pour these plates at least one day in advance...Agar + Antibiotic plates. Prewarm 12 mL recovery media at 37 °C (for at least 15 minutes). Prewarm 3X LB... Agar + Antibiotic plates at 37 °C. Prewarm 8X LB Agar + Antibiotic Bioassay plates. Prechill Micropulser...Micropulser cuvettes on ice. Thaw 4 tubes of electrocompetent cells on ice for 15-20 minutes or until completely...transformed cells on each of the eight bioassay plates (two plates per tube). Distribute evenly with a sterile... edge that can scrape plates more abrasively at a certain angle. Incubate plates upside down at 30 ℃ overnight...

  10. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...improvement over Cas9-mediated HDR for the loci tested, where average HDR-mediated editing frequency was...sequences, expanding the number of available target sites for base editing (Kim et al., 2017). For each base...have an improved base editing capacity, even at sites previously difficult to target. ABE8s can achieve...

  11. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    ...holiday spirit by kicking off our annual #DeckTheLab contest. The bar is set very high from last year’s impressive...some cheer by snapping a photo of you and your labmates celebrating the holidays and tweeting it with ...

  12. Introducing the Addgene Antibodies 101 eBook!

    Type
    Blog Post
    ... first edition of our Antibodies 101 eBook! The latest addition to our eBook collection is a handy resource...start looking for answers.    We hope you find the latest addition to our eBook collection useful. And, as...

  13. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

    Type
    Protocol
    ...existing sites in the original vector. Designing oligos To add NdeI, PacI, AscI and MfeI sites between ...EcoRI and SalI sites of the vector, we design a top oligo with each of the additional sites in tandem ( ... to start at 95°C for 2 minutes. Then, gradually cool to 25°C over 45 minutes. Ligation Dilute 5μL of ...tutorial we will discuss how to add new restriction sites to the MCS of an empty vector. However, the following...set of oligos containing your desired restriction sites and add them to your existing vector. It is a couple...oligo), but this would destroy the EcoRI and SalI sites in the final vector. Order the following oligos ...Place tube in 90-95°C hot block and leave for 3-5 minutes. Remove the hot block from the heat source (turn...

  14. The 12 Days of CRISPR

    Type
    Blog Post
    ...celebrate the 12 Days of CRISPR with some of the latest and greatest in CRISPR tools and resources! Ready to sing...

  16. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    ... spent the last week perusing the Deck The Lab contest submissions on Twitter and Instagram and we’re ...these creative entries as the winners of the 2019 contest. Agar art For the second year in a row, agar ...

  17. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    .... There is value, then, in having a guide that curates this information. And so I created the Codon Guide... genetic engineering curriculum at MIT. He also writes Codon, a blog about biotechnology.    ...

  20. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    ...year with a #DeckTheLab themed nativity scene, courtesy of our house pets.. 😁 pic.twitter.com/qDylF8TdHH... social media throughout the year for more fun contests!  ...
Showing: 601 - 620 of 963 results