We narrowed to 958 results for: Tes
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TypeBlog Post...repository Name Serotype Category Depositor Notes pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources...
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Plasmid Grant to Aid Cervical Cancer Screening in Ghana
TypeBlog Post...screening in Ghana. While pap smears and visual tests are commonly used in Ghana, there is little screening... screening for HR HPV alongside the cytological tests currently in use. Gladys will use plasmids containing... -
Editor's Choice, October 2016
TypeBlog Post...Building Community.” This post leads readers to our latest podcast all about graduate student life and how...myself and two of my previous graduate school classmates, Ben Vincent and Niroshi Senaratne. When we recorded... -
Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service
TypeBlog Post...Plasmids offered as cloning grade DNA As we begin testing out this service, we’re starting with 210 plasmids...next generation sequencing and spectrophotometry to test purity (260/280 nm and 260/230 nm ratios) and measure... -
Congratulations, Deck The Lab winners!
TypeBlog Post...year with a #DeckTheLab themed nativity scene, courtesy of our house pets.. 😁 pic.twitter.com/qDylF8TdHH... social media throughout the year for more fun contests! ... -
Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs
TypeBlog Post...as CRISPR-based gene repression tools to find and test genes that may play a role in the development of...polymerases produced using plasmids from Addgene to test for the presence of pathogens in environmental samples... -
Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering
TypeBlog Post...Golden Gate cloning method to ligate expression cassettes of the gRNAs into a single vector in tandem. They...nickase vectors work successfully to target multiple sites in cultured human cells. While it has not yet been... -
Plasmid Preps: Different Purity, Different Quantities, Different Uses
TypeBlog Post...are used to insert recombinant DNA from prokaryotes or eukaryotes into a synthetic vector that can have ... -
What's New in CRISPR - May 2019
TypeBlog Post...demonstrate efficient editing in adult mice and in zygotes. Read the paper in Molecular Cell Find the Nme2Cas9... was developed by Rebecca Shapiro’s lab. After testing dCas9 fusions with transcriptional repressors, ... -
Your Top Requested Plasmid in 2015!
TypeBlog Post...2015 was... lentiCRISPR V2 LentiCRISPR v2 originates from Feng Zhang’s lab at the Broad Institute ...secretion, and identifying lysine methyltransferase substrates. We’re incredibly happy that lentiCRISPR v2 is... -
6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results
TypeBlog Post... This blog was originally published on BitesizeBio here. As part of my job ensuring plasmid quality at...Verify Plasmids Acknowledgements: Thank you BitesizeBio for originally publishing this and allowing us... -
What's New in CRISPR - Spring 2019
TypeBlog Post...be repurposed for double strand breaks at target sites. Read the paper in Nature Communications Find...is a single step gRNA multiplexing system that generates vectors containing high numbers of gRNAs in one... -
CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping
TypeBlog Post...And could it be a new tool for genome editing? To test the potential of Cas14, the Doudna lab cloned the...of dsDNA and RNA in trans. Fluorescent signal indicates the presence of this viral sequence in the assay... -
Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors
TypeBlog Post...compensation committee. The compensation committee creates a compensation philosophy that helps guide employee...spend time caring for my mother. For fun, I enjoy pilates, jewelry making, reading, sailing, and gardening... -
Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton
TypeBlog Post...nature of the Center for Regenerative Medicine accelerates research. Chapter 5: Research currently under... defines Open Science and how he practices and promotes open science in the lab - sharing reagents, cell... -
Why Do I Need an MTA Anyway?
TypeBlog Post...transfers. The UBMTA was drafted by the National Institutes of Health (NIH) and adopted by the Association... Processing: When you place an order, Addgene generates the MTA and emails it straight to you, or we can... -
Save Time and Money by Making Your Own Competent Cells
TypeBlog Post...being plated on agar with ampicillin selection. Notes and tips We’ve found that our competent cells ...contamination. Also conduct a positive control (test a plasmid that transforms well in other competent... -
Antibody Validation Using the Indirect ELISA Method
TypeProtocol...of our protocols supports reproducibility and accelerates science. Here, we list the specific equipment...instead of room temperature or 37 °C. The protocol notes when there are different incubation options available...empirically determined. If possible, run an initial test with serially diluted primary antibody and determine...Equipment Spectrophotometer compatible with 96-well plates 1–10 µL single channel pipette 2–20 µL single channel...VWR 76322-150 Pipette tips, 20 µL, VWR 76322-134 Pipettes, 10 mL, VWR 89130-898 1 L polystyrene bottle, ...display a green product. A precipitated product indicates the presence of too much HRP and the need to optimize... and incubated overnight with Anti-Desmin [D7]. Plates were incubated with HRP-linked anti-Human IgG secondary... -
Editor's Choice, September 2016
TypeBlog Post...Michael Koeris to learn how his company uses phage to test for Listeria in food products). I picked this post...Plasmids at a Low Price: Kits from Addgene Savvy Advocates Needed to Navigate a Scientific Enterprise in ... -
CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats
TypeBlog Post...might have a few positive control genes on which to test the validity of your biological model and the technical...starting point for developing a model and an assay to test that model. Both libraries are available from Addgene...