We narrowed to 988 results for: igh

...biotin-avidin binding could provide a true measurement of high affinity (i.e. sub-nanomolar) protein-ligand interactions...

...elements Coding sequence features are relatively straightforward to define, but for control elements such as...

...amplify your insert DNA. It is important to use a high fidelity polymerase to minimize mutations. The fidelity...

...Then ask for their honest opinions -- this part might hurt, but if you pitch to enough people, you will...

... and human embryonic stem cells, they found very high recombination percentages (78-79% after two rounds...

...Watson-Crick base pairing, allowing for rational, straightforward design. Since the technique relies on base ...

...knowledge to impart. An undergraduate can mentor a high school student, a graduate student can mentor an...

...spectrophotometer uses the absorbance/transmission of light through a liquid to determine the concentration ...liquid. Molecules absorb different wavelengths of light to varying degrees and most have a specific wavelength... absorbance, you need to know the wavelength of light that your substance maximally absorbs. In the case...

...business consulting, mathematics, and more. That’s right, we have non-traditional scientists working in our...

...back actual magic (which had been lost for an age) fight in the Napoleonic Wars and against something even...

... jGCaMP7c variant in AAV1. This variant exhibits high contrast between its resting state and its calcium-bound...

...and, depending upon who your challenger was, you might have to drastically alter your strategy. I realized...

... Career Scientists, including their "Making the Right Moves: A Practical Guide to Scientifıc Management...

...transfection into mammalian cells is fairly straightforward and the resultant cells can either express ...

... an antibody labeled with Fc, Fab, heavy chain, light chain, constant region, and variable region. ...

...neuroscience with ever more efficient tools for light mediated neuronal control. The advantages of optogenetics...

...makes lentiCRISPR v2 great? It produces a ~10 fold higher titer virus, making it ideal for CRISPR pooled ...

...NHEJ is active throughout the cell cycle and has a higher capacity for repair, as there is no requirement...

...In many cases, CRISPR may be more efficient. You might often start with CRISPR/Cas9, but TALENs do have...

...is important to know that the student presenting right before me had been assigned a structure paper with...

...needs. The most often-cited reasons researchers might not post a preprint are reluctance to share their...

...actually were not many hurdles. It was pretty straightforward surprisingly. So I think a nice thing about...

...limitation of using loxP sites is the inability to tightly control which loxP sites recombine if more than...

...The coregulator ‘core’ complex is composed of a tightly-bound invariant group of proteins, whereas the ...

...education. If you suspect that science communication might be for you, don’t wait. Start communicating now....

...western blot as normal! Stripping your membrane Alright, so you’ve dried your membrane and returned it ...

...your desired genes. Remember Punnett squares from high school biology? They will come in handy here! Crossing...

...for a lay readership, and working with middle and high school students. One of these organizations was ...

...species one is working with, the protocol will slightly differ. For example, Girardia sp. and Dugesia ...

...supplementation with grant funds, money is always tight. Of course, unlike your traditional biology lab,...