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We narrowed to 957 results for: REP

Showing: 621 - 640 of 957 results

  1. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...workforce working group report. http://acd.od.nih.gov/biomedical_research_wgreport.pdf Resources at Addgene...perspective of both grassroots organisations that represent postdocs and institutes that employ them, the ...

  2. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    ...subscription), archived on PubMed Central, or in preprint onBioRXIV. One of the biggest issues you will ...looking for a particular protocol or are trying to replicate an experiment, the methods section probably won... days of file cabinets filled to the brim with reprints and papers with scribbled notations in the margins...

  3. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    ...couple of problems. You couldn’t do it without repeating the same guide RNA twice. The second problem was... created enough regulatory elements, CRISPRs, repressors, activators. We’ve been putting together very...will be available on GitHub, an open source code repository. Addgene: Over the longer term, where is this...

  4. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...important plasmid elements – promoters, origins of replication, protein tags, and antibiotic resistance markers...sequences consisting of two 13-bp long palindromic repeats separated by an 8-bp long asymmetric core spacer...the same or on separate pieces of DNA. Both 13bp repeat sequences on a single loxP site are recognized ...

  6. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...and can be negative and/or even replicated studies to show reproducible results. This a great resource ...targeting vectors that each contains a guide RNA and repair template of your choosing (Schwartz and Jorgensen...

  7. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ....9 kb stuffer sequence that can be removed and replaced with an shRNA sequence of your choice. More information...to produce virus? Get the ready-to-use lentiviral prep from the Addgene viral service. CRISPR technology...together with expression of fluorescent or enzymatic reporters for cellular assays and animal imaging studies...

  9. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...-stranded DNA of ~36kb, with inverted terminal repeats (ITR) present at both ends.   Figure 1:...genes E1 and E3. Without these genes, AdVs cannot replicate on their own but can be produced in E1-containing...this “cytopathic effect.” Amplification can be repeated multiple times (2-4 rounds) at increasing scale...

  10. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...the detection antibody is biotin-conjugated, streptavidin (which has a high affinity for binding biotin...reached 4.0, you can resolve this issue when you repeat the experiment by diluting your standard curve,...of the line. With these tips in mind, you’ll be prepared to run an ELISA in your lab!   References and...

  11. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...protein-coupled receptors (GPCRs), that have been reprogrammed to respond only to inert, nonnative ligands....DREADD-based chemogenetics in mice. Scientific Reports 9: https://doi.org/10.1038/s41598-019-41088-2 Magnus...chemogenetics in our chemogenetics guide Find viral preps for your research Find chemogenetics plasmids for...

  12. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...far more than it was worth to repair the controller and a new replacement was not possible, but one of ...  We’ve also learned to be resourceful and to repurpose old equipment for new uses. We’ll probably never...

  13. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    ...into pieces small enough to be pulled down via streptavidin IP. Biotinylated dCas9 goes along for the ride...the following changes to create CAPTURE 2.0:  Replace the biotinalatable tag on dCas9 with a BioTAP-tag...identified with the multiplexed CAPTURE 2.0 largely replicated interactions previously identified with the original...

  14. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...conference, what worked? Use this information to prepare for the next meeting. Navigating talks Before the...publication, consider uploading your work to a preprint server (and include this information on the poster...later on. Update your LinkedIn and your resume and prepare business cards. You might not consider being a ...

  15. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...the genome and is propagated during normal DNA replication and cell division. Mobile-CRISPRi does not disrupt...measured by knockdown of a constitutely expressed RFP reporter, was variable across bacterial strains, ranging...guides that target essential genes were less represented while bacteria containing guides targeting non-essential...

  16. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...brought into the hood. A 48-well plate is also prepared with about 200 ul of culture medium containing..., and making sure we always maintain a backup replicates of each clone in case of contamination. We do...of single clones obtained (on occasion we have prepared two 48-well plates of clones) which enables investigators...

  17. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...sure cells don't stay out too long While you’re preparing things, make sure your cells don’t stay out too...continued use inadvisable for experiments requiring reproducible results. Most cell culture media contain the...cells stored in their original condition and can replace your working stock if it gets too old or contaminated...

  18. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...complicated if you’d like to use homology directed repair to make precise edits or knock-ins. Editing efficiency...very specific targeting, like homology directed repair and base editing, to more loci. Although these ...CRISPR Base Editing Learn about Homology Directed Repair Resources on Addgene.org Check out our CRISPR...

  19. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...Fig. 1: A schematic representation of Sybr Green chemistry. Blue arrows represent fluorescent signaling...amplicons under 100 bp (Ma, 2015.) A schematic representation of the Taqman reaction can be seen to the left...

  20. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...a three color panel or a 40 color panel! When preparing a spectral flow experiment and panel, there are...first run a new panel on a spectral cytometer, you prepare reference controls of both beads and cells for ...signature upon antibody binding, leading to discrepancies during unmixing. Additionally, it is critical...
Showing: 621 - 640 of 957 results