We narrowed to 972 results for: REP

...our strategy using the pMBP-LbCas12a and pC013-Twinstrep-SUMO-huLwCas13a plasmids from Addgene, which would...a seminar on “Tools to increase scientific reproducibility” by Benoit Giquel, an outreach scientist from...The talk highlighted the importance of reagent repositories and the myriad facilities offered by them which...

...increasing the standard mutation rate seen during DNA replication by 120-fold over background. Due to its modular...bacteria resistant to both spectinomycin and streptomycin, but could also be used for studying epistatic...generate specific mutations in your plasmids Use REPLACER Mutagenesis to mutate your plasmid Resources ...

...HUH-tag; increase homology-directed repair efficiency by linking the repair template VirD2 HUH-tag For creating...Many of these tools can be found in Addgene's repository (Table 1). Figure 2: Adding function to...

... an ERK kinase translocation reporter with a mRuby2 fluorescent reporter (ERK KTR-mRuby2). The ERK KTR...Activity with GFP Resources at Addgene.org Find KTR reporter plasmids Find photoactivatable fluorescent proteins...

...fluorescent biosensor. Scientific Reports,5(1). doi:10.1038/srep12397. PubMed PMID: 26215030. PubMed Central...2008). Rosella: A fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in...

...all viral genes except ITRs (inverted terminal repeats – the sequences that flank the deliverable DNA)...transduced gene is episomal and does not divide and replicate in the same way the host DNA does. Because of ... since you don’t have to wait for the host to replicate the DNA before expression. Now that you’ve got...

...research.   Abhi Aggarwal: Glutamate sensing reporters Abhi Aggarwal is a postbaccalaureate research ...third-generation intensiometric glutamate sensing fluorescent reporter (iGluSnFR3) to study glutamate, a signaling molecule...post-transfection, excited at 505 nm (right). Scalebar represents 100 µm. Image from Abhi Aggarwal.   Brandán...

...contains ccdB. A successful insertion will completely replace ccdB with the investigator’s insert of interest...ccdB SurvivalTM, one can efficiently propagate and prep plasmids containing the ccdB gene, which can then... strains could possibly be used to propagate and prep your ccdB-containing empty backbones but should ...

...community. This is no small task, however, at a repository that consistently receives around 200 new DNA... over 2000 primers. Two 96-well PCR plates are prepared with the primer stocks for forward and reverse...primers that bind within or must read through repetitive regions (such as LTRs or ITRs), hairpins, or ...

...workforce working group report. http://acd.od.nih.gov/biomedical_research_wgreport.pdf Resources at Addgene...perspective of both grassroots organisations that represent postdocs and institutes that employ them, the ...

...subscription), archived on PubMed Central, or in preprint onBioRXIV. One of the biggest issues you will ...looking for a particular protocol or are trying to replicate an experiment, the methods section probably won... days of file cabinets filled to the brim with reprints and papers with scribbled notations in the margins...

...couple of problems. You couldn’t do it without repeating the same guide RNA twice. The second problem was... created enough regulatory elements, CRISPRs, repressors, activators. We’ve been putting together very...will be available on GitHub, an open source code repository. Addgene: Over the longer term, where is this...

...important plasmid elements – promoters, origins of replication, protein tags, and antibiotic resistance markers...sequences consisting of two 13-bp long palindromic repeats separated by an 8-bp long asymmetric core spacer...the same or on separate pieces of DNA. Both 13bp repeat sequences on a single loxP site are recognized ...

...protein-coupled receptors (GPCRs), that have been reprogrammed to respond only to inert, nonnative ligands....DREADD-based chemogenetics in mice. Scientific Reports 9: https://doi.org/10.1038/s41598-019-41088-2 Magnus...chemogenetics in our chemogenetics guide Find viral preps for your research Find chemogenetics plasmids for...

....9 kb stuffer sequence that can be removed and replaced with an shRNA sequence of your choice. More information...to produce virus? Get the ready-to-use lentiviral prep from the Addgene viral service. CRISPR technology...together with expression of fluorescent or enzymatic reporters for cellular assays and animal imaging studies...

...PMM2 gene. Videira’s group recently reviewed and reported that PMM2-CDG patients suffer from immunological...drugs can be used to treat these diseases (drug repositioning). The ultimate goal of this work is to lower... construct NFIX expression vectors as well as reporters of NFIX function. They will then mutagenize the...

...-stranded DNA of ~36kb, with inverted terminal repeats (ITR) present at both ends.   Figure 1:...genes E1 and E3. Without these genes, AdVs cannot replicate on their own but can be produced in E1-containing...this “cytopathic effect.” Amplification can be repeated multiple times (2-4 rounds) at increasing scale...

...hundred nl. Alternatively, for larger brain areas, repeated staggered injections may be considered. Timing...ways how scientists have used Addgene’s plasmid repository and viral service for their behavioral studies...of our viral vectors blog posts Find tips for preparing AAVs View our Beginner's Guide to Viral Vectors...

...and can be negative and/or even replicated studies to show reproducible results. This a great resource ...targeting vectors that each contains a guide RNA and repair template of your choosing (Schwartz and Jorgensen...

...the detection antibody is biotin-conjugated, streptavidin (which has a high affinity for binding biotin...reached 4.0, you can resolve this issue when you repeat the experiment by diluting your standard curve,...of the line. With these tips in mind, you’ll be prepared to run an ELISA in your lab!   References and...