We narrowed to 761 results for: Rest

1. Antibodies 101: Monoclonal Antibodies

   Type

   Blog Post

   ... binds to a specific region of your protein of interest. For example, if you are studying a protein that...

2. Luminescent Imaging with Nano-lanterns

   Type

   Blog Post

   ...and photobleaching. Researchers have long been interested in using luminescence to get around these issues...

3. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

   Type

   Blog Post

   ...Variants Identifies Oncogenic Alleles. Kim E, Ilic N, Shrestha Y. et al. Cancer Discovery 2016. PubMed PMID: ...

4. Multiplex Genome Editing with CRISPR-Cpf1

   Type

   Blog Post

   ...multi-gene editing with a single crRNA array. Are you interested in using Cpf1 multiplexing in your research? ...

5. Addgene’s Viral Service - Why Virus? Why Now?

   Type

   Blog Post

   ... developments on this project and on all the interesting things going on at Addgene. Sharing speeds science...

6. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

   Type

   Blog Post

   ...gRNA and enrich cells for your CRISPR mutant of interest, give these methods a try.  References Højland...

7. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

   Type

   Blog Post

   ...mRNAs and two nuclear long non-coding RNAs. One interesting twist to Cas13d enzymes is their cleavage pattern...

8. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

   Type

   Blog Post

   ...ability to reversibly bind and release a protein of interest in response to light allows for tagless protein...

9. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

   Type

   Blog Post

   ... ZFN require custom nucleases for each gene of interest. CRISPR-Cas9 tools have been used in immunology...

10. Top Requested Lentivirus and AAV of 2016

    Type

    Blog Post

    ...in transgenic animals, where cre expression is restricted to a particular cell type. The human synapsin...

11. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type

    Blog Post

    ...University of Pennsylvania. She is particularly interested in science communication and epigenetics. Follow...

12. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type

    Blog Post

    ...multifragment assembly methods are preferred. No such restriction applies to deletions; using REPLACR-mutagenesis...

13. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type

    Blog Post

    ...share it as soon as possible. Addgene: How much interest are you seeing in this new tool? Mendenhall: We...

14. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type

    Blog Post

    ...great potential for multiplexed genome editing. Interested in using PTG plasmids in your research? Check...

15. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type

    Blog Post

    ...off, to reversibly activate or repress genes of interest. They also “showed that different guide RNAs have...

16. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type

    Blog Post

    ...biotech startup process and work environment! Interested in Synthetic Biology? Read On! Synthetic Biology...

17. Dos & Don'ts When Publishing a Scientific Manuscript

    Type

    Blog Post

    ...whether your manuscript fits the scope and the interest of the journal. Be sure to check the Author Guidelines...

18. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type

    Blog Post

    ...there a senior person whose career path you are interested in following? Someone you admire for their insight...

19. Antibodies 101: Affinity Reagents

    Type

    Blog Post

    ...chains made by immune systems. But here, we're not interested in antibodies. Instead, we're going to focus ...

20. Antibodies 101: Selecting the Right Antibody

    Type

    Blog Post

    ... been validated in the species your protein of interest comes from. If one is not available, don’t lose...