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Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

Type

Blog Post

... ZFN require custom nucleases for each gene of interest. CRISPR-Cas9 tools have been used in immunology...
How to Keep a Lab Notebook for Bioinformatic Analyses

Type

Blog Post

...University of Pennsylvania. She is particularly interested in science communication and epigenetics. Follow...
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

Type

Blog Post

...multifragment assembly methods are preferred. No such restriction applies to deletions; using REPLACR-mutagenesis...
Top Requested Lentivirus and AAV of 2016

Type

Blog Post

...in transgenic animals, where cre expression is restricted to a particular cell type. The human synapsin...
Luminescent Imaging with Nano-lanterns

Type

Blog Post

...and photobleaching. Researchers have long been interested in using luminescence to get around these issues...
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

Type

Blog Post

...Variants Identifies Oncogenic Alleles. Kim E, Ilic N, Shrestha Y. et al. Cancer Discovery 2016. PubMed PMID: ...
Multiplex Genome Editing with CRISPR-Cpf1

Type

Blog Post

...multi-gene editing with a single crRNA array. Are you interested in using Cpf1 multiplexing in your research? ...
Addgene’s Viral Service - Why Virus? Why Now?

Type

Blog Post

... developments on this project and on all the interesting things going on at Addgene. Sharing speeds science...
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

Type

Blog Post

...mRNAs and two nuclear long non-coding RNAs. One interesting twist to Cas13d enzymes is their cleavage pattern...
AAV Q&A with Tim Miles

Type

Blog Post

...understanding that AAV-BR1 has the same genome size restrictions as other AAV. Therefore, one should aim to keep...
Sweating the Small Stuff: Details in the Lab

Type

Blog Post

...experiment. For example, I used to constantly underestimate time needed to prep for an experiment (labeling...
Antibodies 101: Buffers, Storage, and Conjugates

Type

Blog Post

...protein in an experiment with many proteins of interest! Research the brightness of your fluorophores ...
Antibodies 101: Isotypes

Type

Blog Post

...primary antibody but doesn’t bind your antigen of interest. Isotype controls are used to make sure the staining...
Antibodies 101: Monoclonal Antibodies

Type

Blog Post

... binds to a specific region of your protein of interest. For example, if you are studying a protein that...
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

Type

Blog Post

...gRNA and enrich cells for your CRISPR mutant of interest, give these methods a try. References Højland...
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

Type

Blog Post

...ability to reversibly bind and release a protein of interest in response to light allows for tagless protein...
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

Type

Blog Post

...share it as soon as possible. Addgene: How much interest are you seeing in this new tool? Mendenhall: We...
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

Type

Blog Post

...great potential for multiplexed genome editing. Interested in using PTG plasmids in your research? Check...
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

Type

Blog Post

...off, to reversibly activate or repress genes of interest. They also “showed that different guide RNAs have...
Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

Type

Blog Post

...biotech startup process and work environment! Interested in Synthetic Biology? Read On! Synthetic Biology...

Showing: 621 - 640 of 755 results

Results per page:

Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

How to Keep a Lab Notebook for Bioinformatic Analyses

REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

Top Requested Lentivirus and AAV of 2016

Luminescent Imaging with Nano-lanterns

Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

Multiplex Genome Editing with CRISPR-Cpf1

Addgene’s Viral Service - Why Virus? Why Now?

Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

AAV Q&A with Tim Miles

Sweating the Small Stuff: Details in the Lab

Antibodies 101: Buffers, Storage, and Conjugates

Antibodies 101: Isotypes

Antibodies 101: Monoclonal Antibodies

Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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