...research and expanded choice when it comes to ordering high-quality materials from the Addgene repository. Read... be aware that this ordering process will look slightly different from our standard process. We’ll walk... and flexibility; while ensuring we can maintain high standards for both our depositors and requestors...

...In this quarterly blog series, we’ll highlight a few of the new CRISPR plasmids available at Addgene....and human cells. Using the structure of CasX at eight different states of assembly from cryo-electron ...

...efficient capture. We wanted to be able to build a highly multivalent assembly connected by covalent bonds...St. Andrews discovered the domain. It is small, highly stable and has this amazing chemistry locking the... the variation in cancer, not just the CTCs with high marker expression level. Labs have been trying different...

... has been the go-to place. In 2012, his National High Magnetic Field Lab worked with an impressive 1,350... Proteins 101: GFP Fusion Proteins - Making the Right Connection.  mEmerald plasmids and many more! Baird...know how all the parts go together, we can fix it. Right now, we have no idea." Now, it will be that much...

...Cas9, then with the sgRNAs, and can result in a higher titer due to the smaller size of the plasmids. ...of selection. Shalem and Sanjana say they kept a high representation of library within cells and aimed... construct, but in some biological systems one “might need larger numbers.” Further details and protocols...

...Chemistry symposium and a career night. The society also visits high schools to promote chemistry degrees...scientific meetings for students to attend. It also hosts high level academic debates for its members to become...

... available, and have deep tissue penetration and high spatial resolution. By piecing together genes from...are sequentially exposed to acoustic pulses with high enough amplitudes to first collapse arg2 and then...development of ARGs and have already developed a high-throughput screening method for serial acoustic ...

... these results, it’s clear that editing is much higher in the PAM-out configuration. In addition, D10A...D10A editing efficiency is consistently higher than that of H840A, especially with smaller nick distances...cuts close to your insertion site, you can’t obtain high HDR efficiency. Nickases create a staggered cut ...

... Hill. Chromatin immunoprecipitation followed by high-throughput sequencing, ChIP-Seq, is the go-to method... purified for subsequent library preparation and high-throughput sequencing. Improvements to the CUT&RUN...challenging to collect large numbers of cells or when a high degree of signal to noise is desired. It also provides...

...endophyte screening system and plans to employ the light‐inducible bacterial expression system pDawn to clone... India Dr. Mrinalini Nair, Associate Professor (Right), focuses on molecular biotechnology for improving... Rivera Solís, Associate Professor (above, far right), thrives to make affordable biosensors that detect...

... LEGO and science: The potential is endless One night, while opening a LEGO set with my husband, I thought...that resembles a real laboratory. It only took one night to build the first model, but because we are [email protected]. Who knows, your story might just get “legofied.” Many thanks to our guest blogger...

... in signaling processes including smell, taste, sight, inflammation, and neurotransmission. But with approximately...activated downstream of GPCRs that are not involved in sight (Olsen RHJ et al., 2020). A transducer complex consists...Gγ-GFP2 will be in close association, resulting in a high level of GFP2 emission. Following activation in ...

...protocol, you might have noticed that there are many options to elute your DNA prep. You might see protocols...denature, break, and even change the DNA sequence. High pH favors the breakage of the hydrogen bonds, destabilizing...

...knowledge base extensively, you open doors to fresh insights and broaden your understanding, ultimately enhancing...areas in need of improvement. These unrelated ideas might hold the key to unlocking new perspectives or even...to leave a little space for the unexpected — it might just be the key to unlocking the next frontier of...

...communicated the science in my blog posts. This post highlights several of these free tools which will hopefully... selecting “Tools” and then clicking the “Usage rights” dropdown menu, you can search for images “Labeled...

...arrive. Getting off the plane in Bozeman Sunday night, it was easy to spot meeting participants. They ...the ones holding poster tubes (or as our baffled flight attendant called them, "long, skinny things") and...thinking I was an engineer. The talks we heard Monday night represented engineering first and foremost, from...

... allow his team to work mostly at home. Having a high degree of freedom and diversity built into their...new things, diversity of responsibilities, which might include training or teaching others, research, and...sometimes taken to an extreme by scientists) Are highly mobile and can move to a new workplace if opportunities...

...telling it another way. You’ll know you are on the right track if they start asking you questions. Remember...Escape from the Ivory Tower.”  9)   If you think you might want to get serious about science writing, look ...News. Ask your institutional news office if there might be opportunities to blog or write for them. 10)  ...

...bacteriostatic reagents can kill bacteria when used at high concentrations (and vice versa). Looking around ...Antibiotics vary in their sensitivitly to heat and/or light – do not add them to media hotter than about 55oC...55oC and store plates/stocks wrapped in foil if a light-sensitive antibiotic like Tetracycline is used. ...

...pBR322, pACYC, and pUC were developed to provide higher copy number vectors that could be used in these...updated and added new content to our eBook. Some highlights of the 2nd edition eBook include: new chapters...