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We narrowed to 876 results for: NSI;

Showing: 641 - 660 of 876 results

  1. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...plasmids tend to be high copy number. Here, we will consider the replication mechanism of plasmids under stringent...plasmid to balance the total plasmid number.  Let’s consider pSC101. This plasmid is stringently controlled...

  2. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...editing field by making experimental workflows considerably easier, faster, and more efficient than previous...novice to expert-level researchers in achieving consistently successful CRISPR experiments. Jessica is a ...

  3. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...from many other types of cancer. Mesothelioma is considered an orphan cancer; it is rare disease with only...non-resectable mesothelioma by the National Comprehensive Cancer Network, a coalition of the top 27 cancer...

  4. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...technical roles. Therefore, institutions are responsible for ensuring that either all affected scientists...and Brandeis universities have stated that they consider postdocs paid on stipends to be FLSA exempt, and...

  6. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...d like to manipulate in mind. Now it’s time to consider where you would like to attack this gene. Should...sequences into HEK293T cells. The fluorescence intensity was classified into four groups (4; brighter than...

  7. Lentivirus ddPCR Titration

    Type
    Protocol
    ...bleach. Safety Warnings Lentivirus is generally considered biosafety level 2+. Please ensure that you are... media and 11.1 µg/mL Polybrene. Mix the cell suspension well before seeding. Mix each well with a 1 mL...45° angle. Count to 20 while slowly and gently dispensing the droplets down the side of the tube. Place... 95 10 2 1 Denaturation 94 0.5 2 40 Annealing/Extension 60 1 2 40 Enzyme Deactivation 98 10 2 1 Hold 4...

  9. Five Popular Model Organisms

    Type
    Blog Post
    ...great model organism because they are easy and inexpensive to grow and maintain in the lab, have a short... worm, Caenorhabditis elegans, has been used extensively as a model organism since the 1970s when Sydney...

  10. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...this interaction will give you an idea of how responsive and supportive the ELN team is. Once you’ve made...information. If an employee leaves the institution, the transition is smooth as all data is organized within an ...

  12. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ... Summers, 1998). Multimeric plasmids are also considered to be less stable and more prone to being lost...the supercoiled plasmid monomer, and at <6 kb, consistent with the size of a supercoiled plasmid dimer....

  13. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...into the mouse zygote (Qin et al., 2015).  When inside the zygotes, the gRNA will seek out its target ... Ong ST, Bergstrom DE (2018) Viable Mice with Extensive Gene Humanization (25-kbp) Created Using Embryonic...

  14. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ... that have negative impacts on fitness. One consideration when making a transgenic cell line with the ...the piggyBac™ system, these challenges can be considerably reduced. The piggyBac™ transposon system can...

  16. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...training. Similarly, there are several factors to consider when selecting a postdoc mentor and lab. These... having sufficient data to write a manuscript. Consider questions like: What figures are missing? For ...

  18. Colony Formation Titering Assay

    Type
    Protocol
    ... in the lab by heating to 56 °C for 30 min. Considerations Before You Start The health of the target cell...pipetting or inverting. Aliquot 1.35 mL of the cell suspension into each well of the 6-well dish directly on... 48–72 h. Pro-Tip Different cells may be more sensitive to lentivirus or to polybrene, in which case this...

  19. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ... In research, iodixanol is used as an isomolar density gradient medium suitable for virus purification...to mammalian cells. Therefore, and unlike other density gradient media, it is reportedly not necessary ...steps are used to remove contaminants with lower densities, including empty capsids. The 60% step serves ...
Showing: 641 - 660 of 876 results