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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,018 results for: PLE;

Showing: 641 - 660 of 1018 results

  2. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    ...molecular biology and scientific research. There are multiple ways foreign DNA can be introduced into cells ...plasmid can give them different phenotypes. For example, this plasmid encodes a green fluorescent protein...eukaryotic cells via a virus or viral vector. One example are bacteriophages that attach to bacterial membranes...References Baltrus, David A., and Karen Guillemin. "Multiple phases of competence occur during the Helicobacter...

  3. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...was not at all the case. The conference featured people who work in sociology, behavior, and artificial...versa. Even within the biological sciences, there’s plenty of interaction between neuroscience and other fields...microscopes, and dissection tools, but there were also people ready to discuss their VR helmets, gait tracking...field Not to toot our own horn too much, but many people who came to the Addgene booth (there were over ...

  4. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...conditions in order to maximize the mutation rate. For example the Phage Assisted Continuous Evolution (PACE) ...diversity introduced by lambda red recombineering and Multiplex Automated Genome Engineering (MAGE) will always...interest. A gRNA is used to direct the PolI3M-nCas9 complex to a DNA site of interest, which nCas9 nicks and...progeny of a single starting cell. Additionally, multiplexing of guides allows EvolvR to target more than ...

  5. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...primer known as a megaprimer. Piecing together multiple PCR products using sequence homology is also common... Cloning, and SLIC can all be used to assemble multiple fragments, but these methods can be expensive ...could make the E. coli do the work by using the principles of recombineering. In recombineering, bacteria...other methods, such “BAC shaving” would require multiple steps. One potential pitfall of REPLACR-mutagenesis...

  6. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...this complex holds a pair of DNA ends together, forming a paired end complex The paired end complex then... factors can also influence NHEJ activity. For example, repressing RecA and overexpressing the NHEJ machinery...

  7. Addgene: The First Twenty Years

    Type
    Blog Post
    ...plasmids between scientists. The service would allow people to add a gene to a repository others could request... had 1,000 depositing scientists. Ensuring that people knew how to access Addgene, and that we were making...which continue to grow today. “I generally refer people to Addgene for the basics of molecular biology ...reagents, and we realized we could use the same principles to provide recombinant antibodies, another plasmid-based...

  8. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ... when scientists talk about their precious RNA samples. RNA is inherently less stable than most macromolecules...RNA. To protect the RNA from degradation, several simple methods have been employed which modify the sugar...suggest coupling several of them together, for example phosphorothioates with 2’-O-methyl modifications...RNA to control the entire editing system with a simple LED light. Lighting up your gRNA with fluorescent...

  9. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    ...promoter region, which is predicted to be required to decouple gene expression and plasmid number. In a suite...fluctuations. When cells are dividing rapidly, multiple rounds of replication are initiated before the...greatly impacting the level of gene expression. For example, the synthesis of deoxychromoviridans requires ...products is needed, such as within a multi-protein complex, stabilized promoters can eliminate variability...

  10. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type
    Blog Post
    ...ever like to write about a recent plasmid deposit please sign up here. No time to read? Listen to our Hot...aberrantly activate the proteasome. Clift et al. tested multiple methods for TRIM-Away delivery. For small cell...rapid and reversible recruitment of chromatin complexes to any genomic locus in any cell type. By varying...FIRE-Cas9, experiments can be designed to target multiple loci at once. In addition, the approach allows...

  11. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ... channels and pumps in developmental biology.  Examples of some ion-channel and ion-pump encoding genes...genes HCN1 KCNJ2 KCNJ13 GABRG1 KCNIP2 KCNQ1 Examples of some genes encoding gap junction proteins cx43...also modulating bioelectric states in vivo. For example, the Levin lab from Tufts University has used Arch...sensitive fluorescent voltage indicator with near-complete desensitization in continuous light. ArcLight ...

  13. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    ...accessible, anyone could present the material. For example, Addgene’s contributions by Tyler Ford included... and matched to serve a specific audience. For example, I joined this effort later in the year and in ...diverse and international team assembled the right people at the right time. Be inclusive and seek solutions...like to join or contribute to this collaboration, please check out the official website, follow the group...

  14. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...service, and more For the first time in the last couple of years, we don’t have to worry about having enough...community is loving it. Almost 10,000 viral vector samples were distributed in 2018 and we are poised for ...unsolicited praise: “Addgene, for one, is a sterling example of collaboration and innovation between legal and...Our long reaching reputation helps us hire great people from farther away via connections and even via ...

  15. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...on the symposium as a whole and for registration please go to http://futureofresearch.org/boston/. The ... about how the system operates and functions” “Complete lack of information on number of postdocs” “Postdocs...comments are well correlated with published data. For example, in 2010, less than 15% of postdocs had achieved...beginning to address these issues independently; for example, Harvard’s Therapeutics Graduate Program has a ...

  16. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    ...enzymes   The solution to the PAM problem seems simple, in theory: find a Cas enzyme or evolve one that...Cas proteins that don’t utilize NGG   There are plenty of Cas9 variants which utilize alternative PAMs...engineering of the PAM-interacting domain of Cas9. After multiple rounds of engineering, they developed the SpRY...display higher off-target effects than SpCas9, a pleasant surprise since the PAM flexibility could in theory...

  17. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type
    Blog Post
    ... around the world to detect, purify, quantify, deplete, and visualize proteins of interest (Greenfield...antibody expression. If starting from a protein sample (for instance, affinity purified antibodies from...recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. eLife 8: . https://doi.org...NH, Rahman MZ, He L, Xin L, Shan B, Li M (2016) Complete De Novo Assembly of Monoclonal Antibody Sequences...

  19. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type
    Blog Post
    ... into functional multi-subunit macromolecular complexes, but a serendipitous discovery led them to investigate...different Arg-mutant enzymes could be rescued by supplementation with guanidine hydrochloride (GdnHCl). Rescue...previous approval by the FDA, GdnHCl could, in principle, provide an effective treatment for rare diseases...concentrations (≤3 mM) of GdnHCl can functionally rescue multiple Arg-mutant proteins in living baker’s yeast cells...

  20. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...EnvA receptor (TVA) in user-defined cells. For example, a researcher can deliver Cre-dependent TVA to ...optogenetic or chemogenetic receptors) enables complex control of specifically defined neuronal populations...how newly generated neurons integrate into the complex pre-existing circuit of the brain. Another study...circuitry, and continue to contribute answers to the complex questions of how the brain operates. References...
Showing: 641 - 660 of 1018 results