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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 960 results for: REP

Showing: 641 - 660 of 960 results

  1. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...sure cells don't stay out too long While you’re preparing things, make sure your cells don’t stay out too...continued use inadvisable for experiments requiring reproducible results. Most cell culture media contain the...cells stored in their original condition and can replace your working stock if it gets too old or contaminated...

  2. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    ... cell or its genetic material is damaged beyond repair, that cell will most often commit itself to the...Platform (Life Technologies, Carlsbad, CA) that represents 180 genes from the Ras signaling pathway. Its...commonly found across all TCGA tumor samples is represented, making the results of any such study as clinically...

  3. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...Researchers from a variety of institutions recently reported their inability to recapitulate the results of...those of the guest blogger and do not necessarily represent the views of Addgene. Addgene performs Sanger ...greater efficacy, researchers will have to carry out repeated transfections with the ssDNA guide, although many...

  4. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    ...gene construct, you don’t have to worry about repeating elements anymore. The kit contains sequences for...about the advantages of talking to people and representing yourself as a real person, so that students ...doing. They look at structure, impediments, the representation of women on committees, and how the culture...

  5. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...the name suggests, you may also concentrate your prep to the desired concentration using these columns... this assay will also measure the purity of your prep. In this method, antibodies are separated by size...Contaminating bands lower the purity of your antibody prep. If you have multiple contaminating bands or a few...

  6. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    ...evil. However, it is easy to do sloppy science, misrepresent science, or misuse science, and that is what...of creating a technology that could eventually replace scientists themselves.  The sections on optogenetics...12 years, I’m very familiar with the role our repository has played in these fields, and seeing names ...

  7. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...diseases and metabolic diseases are also underway and reported results are encouraging. Importantly, a significant...combined with AAV for in vivo gene editing, and reports suggest that the first clinical study combining... Find AAV plasmids Find ready-to-use AAV viral preps Browse all viral vectors ...

  9. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ...get Addgene from a concept to an up and running repository. As they built the foundation for what Addgene... part about working at Addgene?” Most Addgenies reply with an enthusiastic, “Our company culture! Addgene...things!!”  Okay - honestly, maybe not everyone replies with quite so much animation, but the gist is a...

  10. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    ...become a key focus in recent years. TnpB nucleases represent an exciting class of compact genome editors, typically...TEEP — a tool based on animal data. Step 2: Prepare Oligos Oligo 1: Add 'tcaa' to the 5′ end of your... cells. Conclusion & Future Directions TnpB represents a new generation of compact, efficient, and versatile...

  11. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ...fragment can be used directly in your assemblies. Preparing reagents Keep it cool You’ve done all the learning...number of assemblies, you will also likely need to miniprep a lot of plasmids from your bacterial stocks beforehand...protocol shared by Dr. Rob Hurt for high-throughput minipreps in 96-well plates. Plate wisely You will likely...

  12. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...interpret the results, and develop a successful, reproducible assay. That information can make you a much ...email. Notes from real-life hiring managers In preparation for updating this post, I took an informal poll...applicants. In short, while a cover letter can’t replace skills and experience, it can help the hiring manager...

  13. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...encountering flow data in the literature. Data reported from flow cytometry experiments can be a little...from broadest to most specific marker, and are represented as a series of dot plots in the same order. However...2018, uses quadrants to gate and has numbers representing the percentage of cells in each gate. Used under...

  14. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...option.  Target protein/antibody interactions To prepare for an IP, samples are typically lysed in a non-denaturing...protein/capture antibody intact. The wash step is repeated several times to ensure non-specific proteins ... (1984) Purification and some properties of streptococcal protein G, a novel IgG-binding reagent. J Immunol...

  15. Lab to Office Culture Shock

    Type
    Blog Post
    ...transition from the bench can be jarring for the unprepared, and unfortunately, there aren’t a lot of resources...involves a few key features that can be difficult to replicate in other work settings: The sense of being the...question as well then as I would now. I would have to repress laughter if I was asked that question today, because...

  17. Bioinformatics at Addgene

    Type
    Blog Post
    ...Generation Sequencing (NGS) technology. As our repository continues to expand, we realized the need to ...steps within its own container because of the reproducibility containers provide and for convenient compatibility...processes correctly. After this validation check, we repeated our strategy and copied a few lines of our existing...

  18. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ...potentially affect function [5]. We and others have reported that FP cysteine residues form inappropriate disulfide... all, of the FP fusions. Our moxFPs currently represent one of the most inert solutions for imaging in...and Jack Griffith, her research focuses on the replication machinery of Kaposi’s sarcoma-associated herpesvirus...

  19. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...activation of the circuit promotes subsequent, repeated activation), this is an approximation of reward...instills a sense of “reward”), then the animal will repeatedly nose-poke at the active hole [1, 2].    Place...differential participation in reward circuitry." Cell reports 8.6 (2014): 1857-1869. Pubmed PMID: 25242321. Pubmed...

  20. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...your insert of interest, flanked by long terminal repeat (LTR) sequences, which facilitate integration of...(see Figure 2).  Figure 2: Schematic representation of integration process and episomal forms of...dividing cells because they lack an origin of replication (Banasik and McCray, 2010). However, IDLV genomes...
Showing: 641 - 660 of 960 results