We narrowed to 869 results for: SOM

...reach for that silica spin column, stop to consider some ways to purify DNA without a kit. DNA purification...precipitated, dried, and then rehydrated. There are some slight modifications depending on the type of DNA...

..., experiments can be performed in the absence of some viral proteins (compared to viral infection). Therefore...allow for efficient polymerase activity, whereas some ratios result in significantly reduced activity ...

...Researchers also discovered the modular format of some transcriptional activators: that the DNA binding...that use auxotrophic rescue genes as reporters and some that repress instead of activate transcription. ...

... for an organization in a position that required some involvement in the grants process and managing programs...resources. 3. Get a mentor, find your people Find someone who is working or has worked as a Grant Professional...

...talk is quite simple. It's you, a dry erase board, some markers (chalk is rare these days), and a room of..., if you cannot describe the likely outcome with some specifics, the search committee member cannot either...

...compared to wild-type SpCas9, which also displays some cleavage with an NGA PAM. This variant may increase...potentially reversible since there is no genomic edit. Some Cas13 enzymes require a single base protospacer ...

...variety of related bacteria with similar Cas systems. Some of these Cas systems have enzymes small enough to...could likely do it using Prime editing alongside some creative (and significant!) protein engineering....

... organization that is committed to understanding some of the basic principles of life and health and works...E. E., Mahoney, J. T., Kojima, Y., Ding, Y., Somasundaram, S., Miller, J. A., Kalmbach, B. E., Radaelli...

...generate seven new prime editors, PE6a–g, that overcome some current prime limitations and enable impressive ...protein interactions or map a local proteome, but some applications have been challenging or inaccessible...

...cultures are inspected for sterility. Transducibility Some viral vectors are tested in vitro and in vivo for...gene expression and/or function. These data are sometimes reported or posted on the material page for the...

...anyone who has worked with individual plasmids knows, some plasmids amplify well and others don’t. The size...s far better to verify via NGS now than to learn some of your plasmids didn’t amplify after your screen...

...Every few months we highlight some of the new plasmids, antibodies, and viral preps in our repository...number of cell types at different genomic loci — in some cases, over 20-fold higher editing than PEmax. The...

...plasmid can transfer to, replicate in, and persist in. Some plasmids have a narrow host range, while others ...fully understood, but scientists have identified some of the features that confer broad host range. These...

...be affected by many experimental factors. To list some complicating factors for quantifying AAV: qPCR... or if the sample needs to be quantified again. Some of the issues with titrating by qPCR are addressed...

...Creative titles and controversial topics can garner some attention, though in reality much of our research...about your research is not hard either. Here are some resources: ImpactStory, a website that helps researchers...

...the independent variable you are testing and not some other unintended factor. Control plasmids are used...different days, etc, but outside constraints may impose some limitations and you should be cognizant of these...

...them back to wild type), differentiate them into somatic cells affected by the disease and study what’s ...guide RNA binding site works well. However, since some guide RNAs may tolerate a mismatch in the binding...

...immunostaining of the truncated YFP. In Thy1-Brainbow mice, some neurons expressed as many as 16 copies of the injected...cell, even axons of up to 100 microns in length. Some challenges of the Brainbow system are that it does...

...bind discrete subunits or particular subunit pairs. Some of the antibodies in the collection are able to ...hybridoma antibodies developed in the Springer lab. Some are rabbit immunoglobulin G1 (IgG1) chimeric antibodies...

...nucleus, or visualizing DNA instead of proteins. Some stains can diffuse through the cell membrane freely...depending on the location of the targeted markers. Some commercially available fix/perm kits allow for labeling...