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  3. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...Researchers also discovered the modular format of some transcriptional activators: that the DNA binding...that use auxotrophic rescue genes as reporters and some that repress instead of activate transcription. ...

  4. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ... for an organization in a position that required some involvement in the grants process and managing programs...resources. 3. Get a mentor, find your people Find someone who is working or has worked as a Grant Professional...

  6. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...compared to wild-type SpCas9, which also displays some cleavage with an NGA PAM. This variant may increase...potentially reversible since there is no genomic edit. Some Cas13 enzymes require a single base protospacer ...

  7. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...Creative titles and controversial topics can garner some attention, though in reality much of our research...about your research is not hard either. Here are some resources: ImpactStory, a website that helps researchers...

  8. Hot Plasmids - November 2023

    Type
    Blog Post
    ...generate seven new prime editors, PE6a–g, that overcome some current prime limitations and enable impressive ...protein interactions or map a local proteome, but some applications have been challenging or inaccessible...

  9. Viral Production at Addgene

    Type
    Blog Post
    ...cultures are inspected for sterility. Transducibility Some viral vectors are tested in vitro and in vivo for...gene expression and/or function. These data are sometimes reported or posted on the material page for the...

  10. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...anyone who has worked with individual plasmids knows, some plasmids amplify well and others don’t. The size...s far better to verify via NGS now than to learn some of your plasmids didn’t amplify after your screen...

  11. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...talk is quite simple. It's you, a dry erase board, some markers (chalk is rare these days), and a room of..., if you cannot describe the likely outcome with some specifics, the search committee member cannot either...

  12. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...Every few months we highlight some of the new plasmids, antibodies, and viral preps in our repository...number of cell types at different genomic loci — in some cases, over 20-fold higher editing than PEmax. The...

  13. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...plasmid can transfer to, replicate in, and persist in. Some plasmids have a narrow host range, while others ...fully understood, but scientists have identified some of the features that confer broad host range. These...

  14. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...variety of related bacteria with similar Cas systems. Some of these Cas systems have enzymes small enough to...could likely do it using Prime editing alongside some creative (and significant!) protein engineering....

  15. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...the independent variable you are testing and not some other unintended factor. Control plasmids are used...different days, etc, but outside constraints may impose some limitations and you should be cognizant of these...

  18. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...nucleus, or visualizing DNA instead of proteins. Some stains can diffuse through the cell membrane freely...depending on the location of the targeted markers. Some commercially available fix/perm kits allow for labeling...

  19. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...bind discrete subunits or particular subunit pairs. Some of the antibodies in the collection are able to ...hybridoma antibodies developed in the Springer lab. Some are rabbit immunoglobulin G1 (IgG1) chimeric antibodies...

  20. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...configurations can generate functional scFvs; however, some individual scFvs performing better in one configuration... of screening. Created with Biorender.com.   Some phage display libraries screen antibody genes from...
Showing: 641 - 660 of 861 results