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We narrowed to 1,075 results for: TOR

Showing: 641 - 660 of 1075 results

  1. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...to capture ideas, write down certain notes or brainstorm, but we have to admit that paper-based record...means to manage their work, combining data, team, inventory and project management functionalities with experiment... and included hundreds of researchers. The full story is available here. Our questions focused on researchers...all types of data, which enables researchers to store everything in one place. From pictures to excel ...lab (user, date, time, action) are required for regulatory purposes in many labs. Besides this being of ...

  2. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ... 37°C heat block, LED blue light illuminator or UV light illuminator. Approach: Highly similar to the ...equipment, Fluorescent emission reader Approach: FnCas9 Editor Linked Uniform Detection Assay (FELUDA) is a technique...method based on CRISPR-Cas12. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.02.29.971127 Rauch...SARS-CoV-2 Genetic Material. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.04.20.052159 Zhang...

  3. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...genetically encoded fluorescent probe. Several factors contribute to the popularity of GFP including (...organisms and cell types, (ii) no need to add a co-factor, (iii) easy visualization with standard filter ..., proteins that bind endogenous fluorescent co-factors can be used. Examples include the LOV domains that...Kumagai, 2013). Next to these naturally occurring co-factor binding proteins, systems wherein a protein binds...Smaller genetically encoded probes are based on cofactor binding proteins as described above. The smallest...

  4. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...editing approaches for exon skipping. Addgene depositor Charles Gersbach used paired zinc finger nucleases...multiple exons that, when deleted through NHEJ, will restore the dystrophin reading frame. gRNAs bind in intronic...this result, Nelson et al. find that dystrophin restoration is maintained for at least six months. Future...stem cells 2. Remove any immunogenicity of the AAV vector Once delivery has been optimized, it’s important..., Olson EN. Postnatal genome editing partially restores dystrophin expression in a mouse model of muscular...

  5. Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More

    Type
    Blog Post
    ... AAV Vectors for tageting GABA-ergic interneurons. Listen to Our Segment on these new AAV Vectors Hot ...AAV tools for targeting the nervous system, and vectors for zebrafish engineering. You can find additional...Out All of Our Hot Plasmids Articles Browse the Repository  ...

  6. Suggest A Plasmid You'd Like to Find at Addgene

    Type
    Blog Post
    ...for in the repository? Let us know! You can notify us to reach out to potential depositors in two ways...help us reach out to the corresponding potential depositors. 2. Tweet at us! Tweet @Addgene with #suggestaplasmid...depositing, we hope this campaign will expand the repository in ways that will be most useful to you, the ...

  7. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...co-ops to our directors, we can't celebrate Addgene without recognizing our incredible depositors and requestors...and CSO Melina Fan's words of welcome! Executive Director Chonnettia Jones and Board Member Joanna Brownstein...

  8. Addgene at SfN23!

    Type
    Blog Post
    ...have an antibody specialist, Amrita, and a viral vector specialist, Rebecca. They will be working with ...tackle any experimental questions you may have. Our Director of Content, Brook, and our scientific curation...curation manager, Angela, experts on the Addgene repository, are also attending! Finally, our team is rounded...about Addgene's ready-to-use antibodies, viral vectors, and, of course, plasmids! Plus, we'll be giving...

  9. A Control for All Seasons

    Type
    Blog Post
    ...studying the effect of a new tau protein kinase inhibitor on slowing diseases progression in an Alzheimer..., the experimental group, with the new kinase inhibitor and a second group, the positive control group...protocol but does not receive either protein kinase inhibitor. Since the negative control group does not receive...If one were studying the effects of tau kinase inhibitors on mice, for example, it would be better to treat...available materials, lethality of knockdown, or other factors, consider using one of the alternative, albeit ...

  10. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...with CRISPR–Cas nucleases, base editors, transposases and prime editors. Nat Biotechnol 38:824–844 . https... are even more exquisite for the so-called base editor Cas9, which makes DNA changes in the absence of...breaks (Rees et al., 2018). For C>T and A>G base editors, the intended edit must be in a 5 - 10 nt window... guest blogger John Doench! John Doench is the Director of R&D in the Genetic Perturbation Platform at...

  11. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    ...and viral vectors containing genetic elements from rabies plasmids to international requestors. We also...rabies materials! Some of the materials in our repository, including rabies plasmids, are export-controlled...approved, allows Addgene to ship plasmids or viral vectors containing sequences from rabies and all other ...

  12. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    ...ground and what the founders hope to see in the repository’s future. If you’ve ever been curious about what...be successful and the importance of lentiviral vectors in ensuring that success Chapter 6 - Developing...distribution!) Chapter 8 - Opportunities to create repositories with other materials Chapter 9 - The Founders...computer systems and networks. Benjie was Addgene's Director of Technology for 7 years, and is now Chief Software...

  13. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    ...Joanne Kamens, Addgene’s executive director, said during our celebratory lunch, it’s hard to picture one ... the scientists who deposited plasmids to the repository and for the scientific sharing community (check... in 2016 and we just reached our 10,000th viral vector shipped! In the upcoming months, we will be moving...

  14. Grad School Advice Part 1: Picking a Lab and a Project

    Type
    Blog Post
    ...? 11:51 - 13:28 : How do you define what a good mentor is? 13:29 - 14:31 : What drives you to do science...the Addgene Blog Get Advice on Choosing a Good Mentor Learn How the Future of Research Looks to Improve... mentorship... mentor...

  15. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ...including vector-insert joining for recombinant plasmid construction (restriction cloning), adaptor ligation...reliably detect single-base differences. The original creators of this technique visualized the ligation product...specific base sequences, but are sensitive to distortions in helix shape (22). Large purine:purine mismatches... the Thermostable Ligase Reaction Temperature Calculator can be used to estimate an appropriate incubation...

  16. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...Meselson-Stahl, Hershey-Chase...Who was I to dispute history? And boy did I make the right decision. If I’d ...kit creation and quality control efforts with depositors; Gizela, the lab member who knows how the physical...responsible for guiding new deposits into the repository, performing quality control, and providing technical...Jefferson dined alone.” There was a time in human history when an intelligent, motivated person really could...it alone. The help of fellow lab members and collaborators will not only get your paper out the door much...

  17. Grad School Advice Part 2: Building Community

    Type
    Blog Post
    ...your community, think hard about picking a good mentor, and begin considering career options early. Tune...Depace's Talk on "Precision and Plasticity in Mentoring" We'd like to thank Niroshi and Ben for taking...the Addgene Blog Get Advice on Choosing a Good Mentor Learn How the Future of Research Looks to Improve... mentor...

  18. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ... the party going! At Addgene, we’ve set-up an exhibitor booth in order to help scientists find the plasmids...benefit from depositing their constructs to the repository, and getting feedback from our community. It ...Zhang was awarded one of this year’s SfN Young Investigator Awards and we couldn’t wait to congratulate ... to help their labs save time and resources by storing and distributing their plasmids with the scientific...

  19. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    ...resources. In 2022 alone, our website saw 2.2 million visitors from 231 countries. Guides and protocols were ...beloved blog received 1.3 million page views from visitors in over 200 countries. We, like everyone around...have many successes to reflect upon, a wealth of history to learn from, and a compelling future to plan ...forward to working with Addgene colleagues, collaborators, partners, and the larger scientific community...

  20. What's New in CRISPR - May 2019

    Type
    Blog Post
    ...editing (SECURE) base editors Keith Joung’s lab recently found that a cytosine base editor (CBE) with rat APOBEC1... tethering of DNA template to Cas9 SECURE base editors Nme2Cas9 CRISPR interference in Candida albicans...Read the paper in Nature Find the SECURE base editors All-in-one AAV delivery of Nme2Cas9 with gRNA ...
Showing: 641 - 660 of 1075 results