Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 704 results for: des.1

Showing: 641 - 660 of 704 results

  1. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ... of low-toxicity RNA editing tools.   Fig. 1 Schematic of luciferase RNA knockdown assays using...The hardest part of any revolutionary discovery, including CRISPR, is turning potential into impact. ...two pieces: a single protein, computationally described as a fusion of four Cas7 proteins and a putative...RNA editing methods — streamlining experimental design and its potential for clinical applications. Specifically...high-fidelity rate. Both Cas7-11 and PASTE open up multitudes of possibilities at the bench and potentially...

  2. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ...processed since the declaration, only a small fraction (<1%) of authors have asked for advice about the scope...the time of manuscript submission. The rationale includes increasing the ability of reviewers (and, once...issue by requiring ever more condensed methods descriptions, legends and limiting numbers of figures such...could be mitigated by on-line services such as SlideShare, Figshare and Dataverse. Just as Addgene helps... raise the alpha isoform of GSK-3 to the same (deserved) stature as the beta isoform. Follow him on Twitter...

  3. Protocol - How to Design Primers

    Type
    Protocol
    ...fragment that needs to be amplified should be within 1-10 kB in size. The structure of the primer should ...18-24 bases 40-60% G/C content Start and end with 1-2 G/C pairs Melting temperature (Tm) of 50-60°C Primer... Protocols Designing Primers How to Design a Primer You may also like...Tips on designing primers for PCR Protocols...Cloning by PCR Agarose Gel Electrophoresis Primer Design for PCR Oligonucleotide primers are necessary when... when running a PCR reaction. One needs to design primers that are complementary to the template region.... They are synthesized chemically by joining nucleotides together. One must selectively block and unblock...

  4. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...can make this fun activity relate to STEM.” Figure 1 shows the activities we had for my school’s Math &... can teach  5-10 year olds in a single evening. Grades K-5 cover a broad range of attention spans, skill...CRISPR work? This section was essentially background slides getting everyone on the same page in terms of the... by running it by the teachers and sharing some slides on Twitter, where I was able to get some great ...

  5. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...antibiotic selection. Essentially, this gene is a 2-in-1 providing both bacterial and eukaryotic selection,...The NPTII gene confers resistance to several aminoglycosides, including G418 which is commonly used for ...DNA and generating DNA breaks. The Sh ble gene provides zeocin resistance by expressing a protein that...experiments in which off-target mutations are undesirable. Pros: Works across many species/systems. Cons...

  6. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    ...two (Knutson et al., 2010a,b).      Fig. 1: Undergraduates in Provost’s CURE prepare to analyze...semester one and then proceed to use those skills to design and carry out their own novel experiments in semester...resources, offers mentoring for instructors, and provides opportunities for students to exchange ideas and...results in student learning gains and positive STEM attitudes in CUREs. CBE Life Sciences Education. 21(4):ar74...

  7. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...antibody bonding strengths.    . Steps of an IP: 1) Protein extracts from cells or tissues contain a ...most situations.  A slightly different method is desirable if the antibody is expected to bind weakly to ...vary in shape and structure. Their porous surface provides a large area for binding, but antibodies conjugated... slightly higher, than those of agarose beads, despite the size difference. Magnetic beads are particularly...

  8. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...your virus. Let’s start there!     Figure 1: Flowchart showing the process for using pooled lentiviral... If not, our protocol on Lentivirus Production provides a general approach for producing lentivirus in...excellent protocol paper by the Feng Zhang lab describing construction, lentiviral production, transduction...the Broad Institute- A variety of protocols and guides regarding pooled libraries, from cloning a pooled...

  9. Transferable Skills: Negotiation

    Type
    Blog Post
    ...dollar contracts behind closed doors. Fig. 1: The mythical land of negotiations.    You negotiate...specific details about the individuals, each example provides a scenario where both people get something valuable...

  10. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...successful and meaningful career in the grants profession: 1. Build on your existing skills & passion  Skills such...LLC, a grant professional development firm that includes the social media community GrantChat. She has ... from administration and fundraising to program design and evaluation. She and her team at J. Miller &...

  11. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ... such a popular model organism? Zebrafish (Figure 1) were initially recognized as a useful vertebrate ...deactivation method in zebrafish are short, modified oligonucleotides called morpholinos. When injected into zygotes...CRISPR/Cas9-mediated insertion of foreign DNA. Despite these caveats, several features of zebrafish—including...

  12. Viral Production at Addgene

    Type
    Blog Post
    ...determine purity of the AAV preparation. Figure 1: Silver staining of purified and non-purified AAV ...protocols, titering methods, and quality control are described below. AAV Production AAV distributed by Addgene...) are generated from lentiviral preparations (described above) that are subject to a concentration step...

  13. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...blood test for HMGB1, the high mobility group box 1 protein. Normally localized to the nucleus, if the...tumors.  This process is not quick. It can take decades from the first exposure to asbestos to the time...T-cells to more easily recognize and cause the destruction of cancer cells. Pembrolizumab has shown some...

  14. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...again. Here’s that process!   Figure 1: The Design-Build-Test-Learn cycle adapted for video making...Good as a WFH desk, and as a video control center.   Cycle 2: Zooming Forward Design: We produced ... So, much like SynBio, it was time to start our Design-Build-Test-Learn cycles. If you’re not familiar...through. I know cameras, but we needed someone else. Design: Jennifer thankfully volunteered, and we decided...here, the operator is in a different room.   Design: The previous cycle made me realize we needed to...restrictions in place, most importantly, masks.  Besides the muffled voices beneath masks, humans rely on...reach for Addgene.  So, onto research! I needed to design a system that would allow me to set up a camera...

  15. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...commandments for making it all work – most of the time. #1 Thou shalt find enjoyment in your work If you are ...that extra two miles which is way more fun. And despite those who may wish to tell you so – towel corners...

  16. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...first foray into Reddit. Here are some reasons why: 1. Reddit has a large and active community of people...They also host AMA forums where users can ask designated scientists anything about a particular topic....calendar invite over to the r/Science team with a description of our AMA and Joanne’s preferred time to start...was writing all day and straight on through her designated two hours for answering questions. She was able...toxic advisors, fruitless job searches, and even despair at having chosen an academic past. I hope I was...receive via email. The cool features are too many to describe here, but I have over 2,000 notes which Evernote...

  17. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...email Ryan at [email protected]   References 1. Larivière, Vincent, Yves Gingras, and Éric Archambault.... After months, or years, of grueling effort to design, fund, and conduct a research project, the vast...provide more than enough time for researchers to describe their science, and for viewers to decide if reading...is of interest. In his research, Scott Spicer concludes that “… the emergence of the video abstract genre...websites). WeShareScience is a social platform that provides links to the videos, keeping the copyright and...

  18. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...levels of DnaA to get the job done.   Figure 1: DNA melting by DnaA and IHF is the first step of ...replicate—but not always to the same extent. As described in our previous Origin of Replication post, DNA...replication. For example, the plasmid ColIb-P9 encodes the protein RepZ to drive its own replication. ...

  19. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...conference for any reason was a Singleton. Figure 1: The Binning Singletons logo. Image: James et al.,...was that kid who used recycled notebook paper, despite the fact that it was gray. He has worked in molecular...molecular and microbiology for the last two decades as a staff scientist, but before going to graduate ...

  20. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ... panel design. Find streptavidin plasmids and biotin plasmids at Addgene!     Figure 1: Indirect... this system is increased flexibility for panel design. Another perk is that one primary antibody can ...for example, a previously induced gene knockout. Besides amine- and DNA-binding dyes, nucleoside analogs...walk through the topics we covered in this post. Besides direct labeling, indirect labeling can be used ...surface, dump gating is a useful way to get rid of undesired cell populations. Dump gating uses the same color...
Showing: 641 - 660 of 704 results