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  1. New CRISPR Tools: Cas7-11 and PASTE

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    Blog Post
    ...integrases bioRxiv 2021. 11.01.466786; doi: https://doi.org/10.1101/2021.11.01.466786 Additional Resources ... With Cas13  CRISPR Cheat Sheet Resources at Addgene.org CRISPR Guide...
  2. Transferable Skills Guide: Creativity

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    Blog Post
    ...Review. Retrieved January 14, 2022, from https://hbr.org/2019/11/5-mental-mistakes-that-kill-your-productivity...Review. Retrieved January 14, 2022, from https://hbr.org/2019/07/a-brief-exercise-to-spur-innovation-on-...
  3. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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    Blog Post
    ...toxins to claim territory or kill competing microorganisms, but did you know bacteria also produce substances...about knockout/knock-in plasmids Resources on Addgene.org Check out our molecular biology reference Search...
  4. Hot Plasmids - November 2023

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    Blog Post
    ...design. Nature Communications, 14(1), 5141. https://doi.org/10.1038/s41467-023-40788-8. PMID 37612276.   Optogenetic...proximity labeling experiments in cells, tissues, and organisms. Lee, S.Y., Cheah, J.S., et al. (2023). Engineered...
  5. Viral Production at Addgene

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    Blog Post
    ...Gene Therapy Methods, 25(2), Article 2. https://doi.org/10.1089/hgtb.2013.131 Wang, Y., Bergelson, S., ...Human Gene Therapy Methods, 29(2), 96–103. https://doi.org/10.1089/hgtb.2017.198  ...
  6. Tips for Getting a Faculty Position

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    Blog Post
    ...please do not hesitate to email me at snappe@janelia.hhmi.org." You can find his original blog post below... Series Science Career Options Resources on Addgene.org Learn more About Careers at Addgene ...
  7. CRISPR 101: Homology Directed Repair

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    Blog Post
    ...). PubMed. Making designer mutants in model organisms. Peng, Y. et al. Development 141; (2014). PubMed...CRISPR Editing in Human Cells Resources on Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs...
  8. Harnessing Bacterial Toxins for Allelic Exchange

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    Blog Post
    ...s toolbox. Depending on the growth rate of the organism of interest, from the day primers arrive, the ...about Lambda Red recombineering Resources on Addgene.org Find plasmids for allelic exchange Read our ...
  9. Evolution of Lab Techniques

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    Blog Post
    ... abundance. If a scientist wants to know if an organism has a particular gene mutation, southern blotting...Check out Plasmid Cloning by PCR Resources on Addgene.org Check out Our Protocol Pages Find Molecular ...
  10. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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    Blog Post
    ...difficult given that the viral reservoir spans multiple organ systems. Both approaches may prove more successful...Genome-Wide Screens Additional Resources onf Addgene.org Check out Addgene’s newly updated CRISPR Guide...
  11. Plasmids 101: Dimers and Multimers

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    Blog Post
    ... in the comments below or contact us at help@addgene.org. Resources and References References Bacolla...19408315; PMCID: PMC2735187. More resources on Addgene.org  Addgene's Plasmids 101 eBookAddgene's Molecular...
  12. Finding Your Perfect Job After University

    Type
    Blog Post
    ...Luckily, I have worked for a variety of different organizations, so I tend to provide them with an overview ...practices, but can sometimes go unnoticed at large organizations. Another downside to large commercial companies...
  13. Important Considerations When Using AAVs

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    Blog Post
    ..., AAV7, AAV8, AAV9 Adapted from https://www.addgene.org/viral-vectors/aav/aav-guide. Where in the tissue...Viral Vector Elements Additional Resources on Addgene.org Find AAV Vectors for Your Research Get Ready-to-Use...
Showing: 641 - 660 of 949 results