We narrowed to 404 results for: pho;

1. Simplify Cloning with in vivo Assembly

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   Blog Post

   ...unamplifiable’ Check your amplification by gel electrophoresis. In our experience, good amplification after...

2. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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   ...non-radiative transfer for energy between neighboring fluorophores, FRET can be used to detect the inter- and intramolecular...

3. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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   ...Cas9, such as visualizing genomic loci using fluorophore tagged dCas9 may require the use of plasmid or...

4. Plasmids 101: Protein tags

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   ...tag for purification? Or you want to combine a fluorophore with a tag that localizes your protein to the...

5. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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   ...direction (which is not easy to see in colony PCR electrophoresis), is free of undesired mutations, and that ...

6. Antibodies 101: The Four ELISAs and When to Use Them

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   ... as horseradish peroxidase (HRP) or alkaline phosphatase (AP). Fluorescent tags may also be used, but ...

7. Hot Plasmids Spring 2024

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   ...6).   Figure 6: Illustration of split fluorophore assay to test reconstitution via mRNA trans-splicing...

8. Plasmids 101: Broad Host Range Plasmids

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   ...Gluconacetobacter xylinus, Gluconobacter spp., Hyphomicrobium spp., Klebsiella aerogenes, K. pneumoniae, ...

9. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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   Blog Post

   ...will be cleaved resulting in activation of the fluorophore. The fluorescent signal can be used as an indicator...

10. Transferable Skills Guide: Career Planning Resources

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    Blog Post

    ...HR, greatly increasing your chances of getting a phone interview! If you don’t have a particular employer...