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We narrowed to 958 results for: Tes

Showing: 661 - 680 of 958 results

  2. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...stayed to see the Opening Keynote address with Bill Gates. The long line for bag check was definitely worth...for global health and the various initiatives the Gates’ Foundation is involved with was one of the highlights...

  4. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...restriction sites to flank your insert : You can use PCR Based Cloning and add restriction sites to the ends...as long as it is already bounded by restriction sites that are also present in the same orientation on...Analyzer , allow you to identify which restriction sites are present in a given sequence. When selecting ...recipient plasmid as well as a specifically designed test digest later to verify that the insert was cloned...a version of your insert flanked by restriction sites compatible with the recipient plasmid's MCS. However... within your insert. Adding desired restriction sites to your recipient plasmid : You can modify the MCS...

  5. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...dilution is appropriate). Quality of duplicates: Exclude duplicates from analyses if there is more than...-log of the expected titer of the samples being tested. Always include a No Template Control (NTC), i....small aliquot of each standard (enough for 1 or 2 plates) and store at -20 °C. Once a standard is thawed...the assay multiple plasmids containing ITR were tested. Plasmid #59462 is one plasmid that gave reliable...consistent results. Use the recommended plasmid, or test multiple plasmids to find a suitable one. Some labs...second peak at a temperature of ~70–75 °C usually indicates the presence of primer dimers which can increase...

  6. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...repository  Name Serotype Category Depositor  Notes pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources...

  7. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...After a virion infects a cell, the single particle creates many copies of itself, sometimes up to millions...infected cell.) This is in contrast to bacteria and eukaryotes, which use a binary replication process whereby...

  8. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ... Dangerous Viruses Contributed by guest blogger Tessa Cressy from the labs of Dr. Elke Mühlberger and ...Medical School describes how AAVs function and indicates important things to consider if you’re getting...

  9. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...applied for identification of essential MYC binding sites in a wide range of human cells.  A new pooled library...targeting start codons, splice acceptor and donor sites, and introducing premature stop codons.    New...

  11. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...to the lysosome, and degradation of autophagic substrates inside the lysosome. Historically, methods for...the cytosol, this ratio is low; but when Keima accumulates in autolysosomes, this ratio is high. When untargeted...

  14. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...KSQ uses a CRISPR-Cas9 platform to systematically test the function of all 20,000 genes in disease models... of Oncology Target Discovery at the Novartis Institutes for Biomedical Research. Drug development was...

  19. Summer SciComm: Science Storytelling

    Type
    Blog Post
    ...again until you don’t.” As every researcher can attest to, science is nothing if not solving your way ...creativity, changed the outcome of the project, or illustrates the value of the work and then build a story ...
Showing: 661 - 680 of 958 results