Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 768 results for: Dos

Showing: 661 - 680 of 768 results

  5. CRISPR Library Amplification

    Type
    Protocol
    ...total plasmid DNA. Tips and Troubleshooting What do I do if my transformation efficiency is not high enough... Pooled Libraries Molecular Biology Reference How do I process my Addgene pooled library? Introduction...Endura from two separate transformations). Pro-Tip Do not pipette repeatedly or mix when removing SOC containing...Critical Be careful not to rip or shred the agar. Do so by gentle spreading. Some spreaders have a sharp...Maxi Kit (one conical is its own Maxiprep). Critical Do not freeze pellets for later purification. Immediately...colder reagents (not including the recovery media!). Do not proceed with Maxipreps or NGS until adequate ...representation is maintained. Maxipreps - Less is more: Do not overload the Maxipreps as yield can dramatically...

  6. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ... regions communicate with each other and how they do it (i.e. where the signals come from and what implications...migrates through neurons where it can replicate and by doing so spread across several synaptic connections (Ugolini...

  7. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ... given sequence. You want to choose enzymes that: Do not cut within your insert. Are in the desired location...(usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid. Bonus: It is helpful...GAATTCATGTGGCATATCTCGAAGTAC-3'. Many restriction enzymes do not cut DNA efficiently at the end of a linear piece...any 6 bases, but you should ensure that the bases do not result in the formation of a hairpin structure...empty plasmid, you will still have colonies when you do not add ligase. If the colonies are a result of recipient...significantly more colonies when you add ligase. If you do not see any colonies, you should conduct a positive...

  8. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...want to choose enzymes that: Flank your insert, but do not cut within your insert Are in the desired location...(usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid Will result in your...you cannot find enzymes that meet these criteria, do not fear. You have other options, such as: Adding...empty plasmid, you will still have colonies when you do not add ligase. If the colonies are a result of recipient...significantly more colonies when you add ligase. If you do not see any colonies, you should conduct a positive...

  9. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...little excess gel around the band as possible. To do so, it is often important to take the excised band... about DNA quantification here . Tips and FAQ How do you get better resolution of bands? A couple simple...gel comb; or c) loading less DNA in the well. How do you get better separation of bands? If you have similarly...

  11. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...less stable than viruses produced in cell lines that do not express these sugars. (Takeuchi et al., 1997)...

  14. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...allowing co-imaging. And because bioluminescent markers do not need to be excited by an external light source...

  16. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...machinery such as Rep or other initiator proteins, they do not require host proteins for replication and can...spp., Rhizobium spp., Rhodopseudomonas spp., Rhodospirillum spp., Shewanella spp., Thiobacillus spp., Xanthomonas...

  20. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ... extension can usually ensure that the 3’-base(s) do not form secondary structures. The introduction (...
Showing: 661 - 680 of 768 results