We narrowed to 934 results for: Kin
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TypeBlog Post...some people find this hard so get used to it by making it a concrete goal to say “thank you, job well ... they are at risk of being fired and should be looking for a new position. One of the most common mistakes...
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AAV Production in HEK293 Cells
TypeProtocol...After 2 months, discard the tube and thaw a new working stock. 40% POLYETHYLENE GLYCOL (PEG) 8000 solution... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog Post... days or even weeks. Primer design was done painstakingly by hand. Records of plasmid construction were...newly sequenced bacterial or viral strains. We are working on a way for SnapGene users to apply features from... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog Post...experiments, making AAV starts with some plasmids. You just need three plasmids to start making AAV: the... -
Celebrate Open Data Day with Addgene’s AAV Data Hub!
TypeBlog Post... much-needed community resource. Instead of painstakingly searching for experimental details in research...currently still in beta version, so we’re always looking for ways to improve the viewing or data deposition... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog Post...484 variants, including some potential tetramer-breaking mutations. The library was then screened through...excitation at 493 nm and max emission at 509 nm), making it useful in multicolor labeling particularly with... -
New and Upcoming Viral Vectors - Spring 2019
TypeBlog Post...properties (for example, overall brightness or on/off kinetics) that makes them suitable for different applications...with a CAG promoter exhibits high specificity for Purkinje neurons. 105321 AAV1 pGP-AAV-syn-jGCaMP7c... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog Post...sugar may be a more attractive choice for labs looking to improve their AAV yields. Given the wide range...Clathrin-mediated endocytosis and subsequent endo-lysosomal trafficking of adeno-associated virus/phage." Journal of ... -
Antibodies 101: Epitope Tags
TypeBlog Post...application. Depending on the tag, directly imaging or tracking your protein may also be possible, without the...also be removed by treating the protein with enterokinase, which recognizes the 5 amino acids on the C... -
Antibodies 101: Chimeric Antibodies
TypeBlog Post...You are a scientist looking to determine how Protein A and Protein B interact. You read extensive research...Robinson, M. K., Graham, E. T., Sabo, J. P., Owens, R., King, D. J., Norris, S. H., & Scher, D. S. (1994). Isotype... -
What the HEK?
TypeBlog Post... four. No Y chromosome is present in this line, making it genetically female. HEKs are generally cultured... line for you. Are HEKs for you? If you are looking for a human cell line model, HEKs might be a great... -
Addgene Begins Distribution of Recombinant Antibodies
TypeBlog Post...catalog, so that you will know exactly what you are working with. We will also be collecting feedback from ...Resource Identifiers (RRIDs) for each antibody, making it easy for you to determine whether any given ... -
RUBY-Red Siliques
TypeBlog Post... be green, containing the desired mutation but lacking both RUBY and the construct that rescues the mutation...RUBY was integrated into a CRISPR system, then tracking the segregation of the CRISPR construct could ... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog Post...spatio-temporal regulation, and is difficult to reverse, making it unsuitable for some types of experiments. (To...arabinose, requires many wash steps to remove arabinose making it difficult to regulate quickly. Arabinose also... -
Summer SciComm: Science Storytelling
TypeBlog Post...by outright stating the big-picture problem, by asking your audience a simple question, like “Have you...ending of the story. At this point you may be thinking “I don’t want to bury my results all the way ... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog Post...Wang, Y., Missinato, M. A., Onuoha, E., Perkins, L. A., Watkins, S. C., . . . Bruchez, M. P. (2016). A ... -
Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors
TypeBlog Post...researchers to utilize and develop these tools by making their own fluorescent protein technologies widely...learn more about Xin and Fatima's work and keep checking back in with the Addgene blog for future award... -
The MTA at Addgene
TypeBlog Post...centralized tracking when biological reagents are shared — both at what time and with whom. Tracking these ... -
New and Upcoming Viral Vectors - June 2019
TypeBlog Post...contrast” variant provides improved detection for tracking large populations of neurons in wide-field imaging...fusion fluorescent proteins can affect DREADD trafficking and localization. The Sternson lab recently deposited... -
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog Post...interpreting human variation at large scales and making personalized medicine a reality,” Chiasson explains...replicate current knowledge, but the sensor can also kinetically outperform current methods. Unger plans to use...