We narrowed to 735 results for: RAN-1

...References 1. Haery, Leila, Ryan C. Thompson, and Thomas D. Gilmore. "Histone acetyltransferases and histone...popular papers, but looked for papers from a wide range of authors. Just start writing When I first started...drafts of the manuscript. This is the time to transform the document into something cohesive-- change ...Tips for Improving Your Next Manuscript 6 Tips for Grant Writing Resources on Addgene.org Check out Our...

...antibody bonding strengths.    . Steps of an IP: 1) Protein extracts from cells or tissues contain a ... protein of interest, such as a sample transiently transfected or stably infected with the protein of ...downregulated, examine a protein’s stability or post-translational modifications, or study how a target protein...experiments using a tagged protein may not be translatable to the endogenous system and will need to be...

... reached labs in over 75 countries. Figure 1: SARS-CoV-2 proteins and other components. Image from...loop-mediated isothermal amplification after reverse transcription (RT-LAMP) and a colorimetric readout that can... 45 minutes. The team deposited the reverse transcriptase, protease, and Bst polymerase constructs from...toolkit include ones for expressing MMLV reverse transcriptase and Taq polymerase that can be used in a one-step...

...two (Knutson et al., 2010a,b).      Fig. 1: Undergraduates in Provost’s CURE prepare to analyze...). But is it possible to bring the kinds of transformative educational experiences afforded to those fortunate... now connects MDH-focused CUREs at 50 schools, ranging from R1 institutions to community colleges, and...NADH production.   CUREs are beginning to transform undergraduate education in the sciences by making...

...commandments for making it all work – most of the time. #1 Thou shalt find enjoyment in your work If you are ...responsibilities. Another tip: don’t miss your grandfather’s 100th birthday, as your mom will never forgive...when you can’t get home, babysitting or running errands. Give help when you have time, and in return you...decent meal, fold their laundry, take public transportation, call a cab and remember their own school assignments...

...to an antigen. AdEasier-1 cells BJ5183 E. coli cells containing the pAdEasy-1 packaging plasmid. Adenovirus...enzyme PmeI . This construct is then transformed into AdEasier-1 cells (Addgene #16399) , which are BJ5183...plasmid pAdEasy-1 (Addgene #16400) is suitable for most purposes. For especially long transgenes, the use of...gene therapy landscape . Signal Transduction and Targeted Therapy, 6 (1), 53. https://doi.org/10.1038/s41392...icosahedral capsids and build new virions. Figure 1: Wild-type adenovirus and its genome. Genes over or...production system. Figure is based on the use of pAdEasy-1. Created with BioRender.com. AdMax™ AdMax™ is another...as a Zika virus vaccine . Scientific Reports, 10 (1), 3613. https://doi.org/10.1038/s41598-020-60238-5...

...for your first journal club to begin! Figure 1: Discussing next-generation sequencing at the Addgene...of current new and exciting literature. As I transitioned out of academia and away from the bench I certainly...

...can make this fun activity relate to STEM.” Figure 1 shows the activities we had for my school’s Math &...olds in a single evening. Grades K-5 cover a broad range of attention spans, skill levels, and STEM exposure...aspects of talking to 8th graders was the broad range of questions I received. I gave my presentation ...

...blood test for HMGB1, the high mobility group box 1 protein. Normally localized to the nucleus, if the...of diagnosis) is about 35 years, but it usually ranges from 20-50 years and sometimes longer. There is...be Used to Study Cancer Drug Resistance Plasmid Grant to Aid Cervical Cancer Screening in Ghana Additional...

...testing. You can find Nick on LinkedIn. References 1. Skloot, Rebecca, and Bahni Turpin. The immortal life...cell line contamination: Epithelial Mesenchymal Transition Is Required for Acquisition of Anoikis Resistance... to carry our independent quality control for a range of testing, from animal doping to forensics, DNA...

...Addgene's Chemogenetics Plasmid Collection . Figure 1: General overview of a chemogenetics experiment. Early...bidirectional control of neuronal activity. Table 1: Descriptions of DREADDs and their activity in neurons... while PSAMs and LMOs have an effect for only 0.5–1 hours after delivery. Chemogenetic Ligand The chemogenetic...Causal Cellular and Neuronal Biology. Physiol Rev, 98 (1), 391–418. https://doi.org/10.1152/physrev.00009.2017...Neurophotonics 11 (2), 021005. https://doi.org/10.1117/1.NPh.11.2.021005 PMID: 38450294 Coward, P., Wada, H... Gi-coupled receptor. Proc Natl Acad Sci USA, 95 (1), 352–357. https://doi.org/10.1073/pnas.95.1.352 PMID... 451–460. https://doi.org/10.1007/s00213-004-2017-1 PMID: 15765260 Farrell, M. S., Pei, Y., Wan, Y., Yadav...

... typically ranges from 8–10 kb, encoded on RNA (Figure 1). This RNA is reverse transcribed into the provirus...— contains transgene and LTRs (chimeric 5’ LTR) Packaging plasmid 1 — contains gag and pol Packaging plasmid...pre-infusion CAR T-cell products. Journal of Translational Medicine , 20 (1). https://doi.org/10.1186/s12967-022...been removed or mutated for increased safety. Figure 1: Wild-type lentivirus genome. Created with BioRender.com...genes (for example, vif , vpr , vpu , and nef for HIV-1). The LTRs flank all of these genes, and anything ...and third-generation systems, please refer to Table 1. Figure 4: Third-generation lentiviral plasmids. Created...heterologous enhancer/promoter such as CMV or RSV Table 1. Summary of key differences between second- and third-generation...

...complex. So how exactly does this work? Figure 1: APOBEC- and Cas9-mediated editing (ACE) reporter ... affecting protein translation of different reporter proteins. Protein translation requires the start ...compared to CRISPR/Cas9 edits that rely on double strand DNA breaks. Due to this inefficiency it is crucial...constitutively expressed to identify successfully transfected cells. C-to-T editing by the APOBEC-Cas9 editosome...that results in the restoration of fluorescence. Transient reporter for editing enrichment (TREE) The Wang...Wang and Brafman lab developed a transient reporter for editing enrichment (TREE) to purify single base... to cells segregated solely by a reporter of transfection, which only report the efficiency of plasmid...

...therapies to clinical trials in 2021. Figure 1: Schematic of the human MECP2 locus and MECP2 protein...References: Amir RE, Van den Veyver IB, Wan M, Tran CQ, Francke U, Zoghbi HY (1999) Rett syndrome is caused...collection of molecular biology tools so that both veteran and new Rett researchers can find the reagents ...maturation (Tillotson & Bird, 2019) and is a global transcriptional regulator of thousands of genes. Studies have...have suggested roles in transcriptional repression, activity dependent de-repression, chromatin remodeling...groove of AT-rich DNA sequences to regulate transcription (Baker et al., 2013). Mutations that affect ...gene editing, MECP2 reactivation, RNA editing, RNA trans-splicing, and protein replacement to reverse the...

...levels of DnaA to get the job done.   Figure 1: DNA melting by DnaA and IHF is the first step of ...the proteins cooperatively can separate the DNA strands. Created with BioRender.com. Once DnaA breaks...it recruits helicases to continue unwinding the strands. This creates two replication forks that move in...elongation phase, a DNA polymerase synthesizes a new DNA strand at each replication fork. Termination sequences...act as a feedback mechanism to reduce further transcription. Replication is also regulated by the balance... concentration is controlled at the level of translation. RepZ mRNA must fold into a “pseudoknot” held...intramolecular base pairs for the protein to be translated. An antisense RNA called Inc, also encoded by...

... over again. Here’s that process!   Figure 1: The Design-Build-Test-Learn cycle adapted for video...looking, I found an inexpensive HDMI transmitter that could transmit a signal between a wall. There was ... things first was getting that wireless HDMI transmitter/receiver, to beam the camera’s image to my remote...then set up a monitor connected to the wireless transmitter and an iPad in the office next door (connected...

...first foray into Reddit. Here are some reasons why: 1. Reddit has a large and active community of people... general advice on how to meet people, how to transition out of academia, and other science career topics.... Although I didn’t start answering until 5pm, frankly, I was typing far before five. Fortunately I have...

...gene, and a terminator, as in Figure 1). Next, up to six Level 1 modules can be assembled into a Level...directionally assembled into a Level 1 vector creating a single transcriptional unit (for example, a promoter...coli to maintain the final plasmid after transformation. Figure 1: Overview of the MoClo Toolkit components...consists of three sets of cloning vectors (Level 0, 1, and 2) which can be used in successive assembly steps... mammalian expression vectors for transient or stable transfections. PUF Assembly Kit Mammalian Expression...nucleases (TALENs) and transcription factors (TALE-TFs) for genome editing and transcription modulation applications...multigene constructs. MoClo Plant Parts III: Transformation & Genome Engineering Kit Plant Expression, ...

...cells were transfected using 1:1, 1:2, 1:3 and 1:6 µg of pRosetta :µg of PEI. The 1:2 and 1:3 ratios provided...18.9 18.9 1:2 18.9 37.8 1:3 18.9 56.7 1:4 18.9 75.6 1:5 18.9 94.5 1:6 18.9 113.4 Gently add the diluted PEI...possible range of ratios to test: Ratio of DNA:PEI Amount of DNA (μg) Volume of 1 mg/mL PEI (μL) 1:1 18.9 .... Thawed aliquots should be discarded after 1–2 months. 1 mg/mL polyethylenimine, linear MW 25,000 Da ...stable. Dilute 1:3 (µg DNA:µg PEI) in 500 µL total of OptiPro SFM (per 10 cm plate). 56.7 µL of 1 mg/mL PEI...for viral production) Day 1: Transfect Cells Day 2 (am): 18 h post transfection - Remove media, replace ...prepared, transfect cells with a fluorescent plasmid using a variety of ratios. Check the cells 1–2 days ...

...Communications , 15 (1), 3663. PMID: 38688943 Hsu, P. D., Scott, D. A., Weinstein, J. A., Ran, F. A., Konermann...pooled format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9..., 1163–1171. PMID: 23979020 Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D..., 17 (5), 471–479. PMID: 32203383 Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I....CRISPR-Cas9. Cell , 167 (7), 1829-1838.e9. PMID: 27984730 Ran, F. A., Hsu, P. D., Wright, J., Agarwala, V., Scott...Nature Protocols , 8 (11), 2281–2308. PMID: 24157548 Ran, F. A., Cong, L., Yan, W. X., Scott, D. A., Gootenberg...Communications , 14 (1), 6175. PMID: 37794046 Small precision edits Anzalone, A. V., Randolph, P. B., Davis,...