Addgene

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Showing: 661 - 680 of 706 results

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CRISPR 101: RNA Editing with Cas13

Type

Blog Post

...sites from the 18,385 observed with REPAIRv1 to merely 20. Expanding RNA Editing Capabilities through ...
A Career in Grants: How To Become a Grant Professional

Type

Blog Post

...close-out a grant. Grant professionals also build relationships with foundations and government grantmakers....
Negative Can Be Positive: Open AAV Data with Addgene

Type

Blog Post

... AAV questions every year on a range of issues related to AAV vectors, many from people trying to answer...
Deep Dive: Fixing and Permeabilizing for Immunofluorescence

Type

Blog Post

...maintaining the structure of your samples, the relationships between cellular components, and the accessibility...
MXS Chaining

Type

Blog Post

...CpG dinucleotides in their 6-mer sequences, are rarely found in the transcriptomes of these species and...
Tips for Screening with Yeast Two Hybrid Systems

Type

Blog Post

...set up, conducted and analysed with care to get reliable information. Although two-hybrid systems are a...
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

Type

Blog Post

... of the reasons why we have only three biology-related Nobel prizes, out of which only one was done in...
Your Lentiviral Plasmid FAQs Answered

Type

Blog Post

...Viral components typically total <1.5kb, which is rarely more than 30% of the transfer plasmid. Many transfer...
Hot Plasmids: Fall 2024

Type

Blog Post

...researchers used the reporter to visualize biologically relevant forces at focal adhesions in living cells in several...
Antibodies 101: Immunoprecipitation

Type

Blog Post

...conformations and interactions. For difficult to release proteins, including those in the nucleus, more ...
Viral Vectors 101: Pseudotyping

Type

Blog Post

...Gutierrez-Guerrero, et al, 2020). Because retrovirus is closely related to lentivirus, many retroviral envelope proteins...
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

Type

Blog Post

...muscles of the gut. Embryos that lack bagpipe have severely disrupted visceral mesoderm and subsequently the...
Plasmids 101: Plasmid Incompatibility

Type

Blog Post

...daughter cell during cell division. They don’t simply rely on chance (or random diffusion) to make this happen...
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

Type

Blog Post

...structure with regard to the Mentor-Singleton relationship. Some of the variability was organic, as some...
Hot Plasmids - August 2020

Type

Blog Post

...guide RNA (gRNA) sequences. But these approaches rely on continual expression of both dCas9 and the gRNA...
Plasmids 101: Broad Host Range Plasmids

Type

Blog Post

...aurum, M. smegmatis, Paracoccus denitrificans, Pasteurella multocida, Porphyromonas gingivalis, Proteus ...
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

Type

Blog Post

...containing FLEx technology. Read the genOway press release for more information. References 1. Atasoy, D.,...
CRISPR 101: Targeting RNA with Cas13a (C2c2)

Type

Blog Post

...Class 2, candidate 1, 2, or 3). C2c1 and C2c3 are related to Cpf1, but they require both a tracrRNA and crRNA...
CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

Type

Blog Post

...that the desired edit will be performed on all relevant bases within the editing window, for example all...
Plasmids 101: Gateway Cloning

Type

Blog Post

...their desired DNA sequences. Gateway technology relies on the two reactions described below: The BP Reaction...

Showing: 661 - 680 of 706 results

Results per page:

CRISPR 101: RNA Editing with Cas13

A Career in Grants: How To Become a Grant Professional

Negative Can Be Positive: Open AAV Data with Addgene

Deep Dive: Fixing and Permeabilizing for Immunofluorescence

MXS Chaining

Tips for Screening with Yeast Two Hybrid Systems

Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

Your Lentiviral Plasmid FAQs Answered

Hot Plasmids: Fall 2024

Antibodies 101: Immunoprecipitation

Viral Vectors 101: Pseudotyping

Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

Plasmids 101: Plasmid Incompatibility

Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

Hot Plasmids - August 2020

Plasmids 101: Broad Host Range Plasmids

Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

CRISPR 101: Targeting RNA with Cas13a (C2c2)

CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

Plasmids 101: Gateway Cloning

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