We narrowed to 958 results for: Tes
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TypeBlog Post...been limited to the overexpression of various metabolites in Escherichia coli or Saccharomyces cerevisiae...regulator or the number of designed scaffold binding sites. With hundreds of characterized aptamer domains ...
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Advice for Moving Into Sales After Your Science Postdoc
TypeBlog Post... for marketing positions posted on recruitment websites, this job specifically looked for a researcher...think of to start was “Marketing for Dummies”. The fastest way to learn something about marketing would be... -
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering
TypeBlog Post...neurons at once. Addgene spoke to Boyden about this latest optogenetics advance, the big questions that motivate...neuron or set of neurons, you can see how it contributes to behavior or pathology, and by disabling neurons... -
Protocol - Over-Agar Antibiotic Plating
TypeProtocol... fits the plate. Incubate plates at 37 ℃ for 18 hours. Day 2 Observe plates for colony formation. Shown...Antibiotic Plating You may also like... Making LB Agar Plates Bacterial Transformation Recovering Plasmid DNA...source) Incubator Reagents 6 cm diameter LB/agar plates without antibiotic High concentration (100 mg/mL... the plate at room temperature for at least 30 minutes with the lid on to give the antibiotic time to ... with smaller size than the 1 mg/mL and 2 mg/mL plates and effective selection. Selection Curve of Transformed... -
K. phaffii: Rising to the Occasion
TypeBlog Post...short, K. phaffii is a non-pathogenic yeast that operates as an obligate aerobe. It’s also a methylotropic...has been sequenced for some time, clearing the greatest hurdle for genome engineering. A unique evolutionary... -
Addgene Depositors Get More Citations
TypeBlog Post...Addgene plasmids and those without, we adjust our estimates based on the journal that published the articles... This is also true for other journals, so our estimates now take into account any differences in citations... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog Post... of a 96-well plate. Then, samples from several plates are pooled together to create a single library ... from plexWell™ NGS data. Once Addgene produces plates of plasmid DNA, they are shipped to seqWell, where... -
Science Career Options
TypeBlog Post...writing skills Writing a thesis or dissertation demonstrates that you have advanced writing and formatting...apply for a job, it is useful to search online job websites to see what requirements or qualifications are... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog Post...alleviate this frustration by producing, titering, testing, and providing lentiviral preps of select lentiviral... vectors from the repository for you. We perform test infections with all of our viral preps and host ... -
FPbase: A new community-editable fluorescent protein database
TypeBlog Post...on a web-based interactive visualization of FP attributes (which turned into www.fpvis.org). That page...is also difficult to scale such a resource when updates depend on a single person. With thousands of scientists... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog Post...nearby resulting in fluorescence within tens of minutes (Cabantous et al., 2013). The Shu Lab took advantage...reporter can be used to visualize apoptosis of enterocytes in the midgut of Drosophila, an observation that... -
The Crispr Babies Saga Shows the Need for Action, Not More Delays
TypeBlog Post... all worked in tandem, within their respective mandates, to tackle a controversial and ethically fraught...stages of human clinical trials. This model also demonstrates that when we are at the point of scientifically... -
Early Career Researcher Toolbox: Free Tools for Science Writing
TypeBlog Post...Pomodoro technique, which is when you spend 25 minutes focusing on a task, like writing, and then take...managers, but Mendeley is free to use. You can take notes and highlight the papers saved to your Mendeley ... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog Post... cell. The viral DNA containing the gRNA then integrates into the genome. Thus the gRNAs responsible for...high throughput in vivo model to identify drug candidates that modulate ApoB-LP size and concentration,... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog Post...sequence containing transcription factor binding sites can increase transgene expression and transduction...validate ITR sequences as these enzymes cut at known sites within the ITR. However, small deletions and point... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog Post...layer of both bacteria types. In water, the dye dissociates into CV+ and Chloride (Cl-) ions. When the negatively...the cell purple. When the alcohol is added, it dehydrates the peptidoglycan layer, trapping the large dye... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog Post...under a new promoter each time a new hypothesis is tested. Controlling multiple genes with Gal4/UAS system...Targeted gene expression as a means of altering cell fates and generating dominant phenotypes., Development... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog Post...dynamically rearranging into different polymerization states. Instead, R body monomers respond to pH changes...their cell walls removed with lysozyme). This demonstrates that R bodies can function to break membranes... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog Post... up on a Saturday at like 7 am to go check your plates and you’re excited to do so, you know that that...Addgene has behind it is something that very much resonates with me.” About Addgene’s role in the future,... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog Post...enzymes which cleave the DNA molecules at specific sites and 2) running the reaction on an agarose gel to...To do this, you'll use enzymes with restriction sites that flank the insert. You will need to know both...