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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,025 results for: URE

Showing: 1021 - 1025 of 1025 results

  1. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...Introduction Sequence verification of important plasmid features (such as the gene/insert, fusion proteins, point...sequencing primers, including: Is the primer unique? Make sure the primer only anneals once within the entire construct...mis-called? Open the trace file and use the search feature in the program to locate the incorrect sequence...sequence. Look at the peaks in the area and make sure they are justifiable peaks. For instance, in the trace...

  2. Fluorescence Titering Assay

    Type
    Protocol
    ...generally considered biosafety level 2+. Please ensure that you are in compliance with your institution...Microcentrifuge tubes, Neptune 3745.X 6-well tissue culture treated dish, Corning 3516 15 mL conical tubes,... supernatants to multiple freeze-thaw cycles. Procedure Seed 75,000 cells into each well of a 6-well dish... the average of multiple dilutions. Sample Data Figure 1: 293T cells were transduced with a range of dilutions...

  3. Colony Formation Titering Assay

    Type
    Protocol
    ...Microcentrifuge tubes, Neptune 3745.X 6-well tissue culture treated dish, Corning 3516 15 mL conical tubes,...transduction, and not during the cell seeding step. Procedure Before beginning a colony formation assay, the... the average of multiple dilutions. Sample Data Figure 1: A549 cells were transduced with the indicated...then stained with 0.1% crystal violet and counted. Figure 2: A549 cells were transduced with the indicated...

  4. Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs

    Type
    Protocol
    ...so tight that you cannot perform your duties. Be sure to try out different sizes to know your fit. It ...protocol goes a long way. It is the easiest way to ensure that you’re doing your work in the safest way possible...note that you do so at your own risk; you should ensure that any local guidance is also adhered to. None...

  5. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...amounts) Appropriate restriction enzyme (see manufacturer's instructions for proper amount) Approrpriate...Approrpriate restriction digest buffer (see manufacturer's instructions) Gel loading dye Electrophoresis buffer... dig one out of the freezer and you are not 100% sure it is what you are looking for, but you have a map...
Showing: 1021 - 1025 of 1025 results