Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,052 results for: FER

Showing: 681 - 700 of 1052 results

  1. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...that “… the emergence of the video abstract genre offers a low-barrier opportunity for researchers to leverage...to measure the impact of their contribution. It offers a 5-step process and several examples for creating...professors at the University of Washington in St. Louis, offer guidance on how to make a low-cost whiteboard video...WeShareScience or email Ryan at [email protected]   References 1. Larivière, Vincent, Yves Gingras, and Éric...

  2. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ...of veracity of the conclusions drawn. The main difference with the PLOS policy is that access of this data...perhaps similarly impoverished lab) might apply different or novel tools and assumptions to reveal findings...geneticists may track the motion of multiple animals on different occasions via video, generating huge amounts of...responsible for storage.  If datasets are too large to transfer to a journal repository, they are likely too large...

  3. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    ...nucleotide insertions, dele­tions or other mutations, referred to as ‘indels’. While this is convenient for generating... iPSCs (or correct them back to wild type), differentiate them into somatic cells affected by the disease...iPSC clone picking, a 100-fold decrease is the difference between picking 100 versus 10,000 clones! We ...developing novel iPSC-based model systems.     References 1. Cong, Le, et al. "Multiplex genome engineering...

  4. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...flanking a FLOXed neoR gene. pAAV-SEPT-Acceptor, the preferred vector, contains a promoterless splice acceptor-IRES-neoR...Schofield, 2015; Moser and Hirsch, 2016; Epstein and Schaffer, 2017) Hopefully you’ve been able to check out...cohesin gene inactivation in human cancer.     References 1. Russell, David W., and Roll K. Hirata. "Human...27280971. 10. Epstein, Benjamin E., and David V. Schaffer. "Combining engineered nucleases with adeno-associated...

  5. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...to a mouse model of muscular dystrophy. Three different splice site donor and acceptor sequences were ...reconstituted after integration; and a sequence of stuffer DNA after the gRNA target site. Donor B contains...greater and more consistent transgene expression. References: Allocca M, Doria M, Petrillo M, Colella P, Garcia-Hoyos...retinal gene therapy Learn about the parts of an AAV transfer plasmid Resources on Addgene.org: Check out ...

  6. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...of interest supports a variety of processes in different cell types. One great way to do this is to use...SuperPLD module is optogenetically recruited by different CIB constructs to the plasma membrane or lysosomes...future updates to the collection! Resources and references Additional resources on the Addgene blog Viral....org Optogenetics Guide Optogenetics Plasmids References Aljiboury, A. A., Ingram, E., Krishnan, N., Ononiwu...

  7. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...insertions, like a fluorescent protein knock-in, suffer from low efficiency when a standard dsDNA repair... secondary structure. Today, multiple companies offer long single-stranded DNA production services or ...Quadros et al. attribute much of this variation to differences in guide RNA efficiency. For example, in a two-guide...Quadros for assistance with editing this blog post. References Yang, Hui, et al. "One-step generation of mice...

  8. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...directly into fertilized mouse eggs, circumventing the need for enrichment and selection offered by mouse ...into either the pronucleus or the cytoplasm of fertilized mouse eggs. Or, to avoid handling embryos ex ...therapeutic applications that better human health.     References Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib...

  9. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...nutrient-rich and also the best source for mycoplasma proliferation. Plus, it is added to the media after autoclaving... contact with a nutrient source, they start proliferating shortly. Once they are present in the laboratory...Bioluminescence assay kits are available from different biotech companies which are enzyme-based mycoplasma...and a Bachelor's degree in Biotechnology.       References Degeling MH, Maguire CA, Bovenberg MSS, Tannous...

  10. Finding Your Science Policy Path

    Type
    Blog Post
    ... away from the bench, I have worked for seven different organizations in a variety of science policy roles...cultural, and political. Science policy looks differently depending on how and where you pursue it. It ...you do science policy? Just like there are many different types of science policy careers, there are many...NSPN maintains a great resource guide including conferences, societies, and outreach groups. Grab a virtual...

  11. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...TALENs more specificity and a broader substrate preference than the ZFNs.   Genetic Engineering Era: Post...genetically engineered superhuman Khan. The novel, Inferno (2013) and the movie version (2016) stars Tom Hanks...that are typically associated with the DSBs and offered unprecedented prospects of curing diseases caused...your research Browse plasmids for base editing References Anzalone AV, Randolph PB, Davis JR, Sousa AA,...

  12. Science Guides

    Type
    Guide
    ...Pooled libraries are single preparations of many different plasmids. Plasmids within a given library have...the same backbone, but they express or target different genes. Some libraries cover the majority of the...Find educational guides to learn more about the different types of viruses that are commonly used in research...

  13. Addgene's eBook Collection

    Type
    Guide
    ...you're not sure where to start or need a handy reference, Addgene's eBooks are here to help!...you're not sure where to start or need a handy reference, Addgene's eBooks are here to help! 0 of 0 Selected...and management, resources for identifying your transferable skills, and tips on the job application process...

  14. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    ...Addgene is excited to announce we now offer an export license application for rabies materials! Some ...rabies plasmids to international requestors. We also offer export license applications for VSV and SARS-COV...before your account is charged.  Timelines  We have offered VSV and SARS-COV-1 plasmids under this application...

  15. Call for Guest Bloggers

    Type
    Blog Post
    ...has deposited If you’d like to write about a different topic, feel free let us know. What do you get ...audience of biological scientists from a variety of different fields. Exposure - With over 100,000 views each...interact with and discuss your work with many different people. Recent Guest Writers Have Included: Wenning...

  16. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    ...search engines, which allow you to search many different antibody catalogs at the same time.  If you're...Library for Cancer Research Human Luekocyte Differentiation Antigen Workshops Immune cells Flow cytometry...others) Data repository From the Human Cell Differentiation Molecules group Addgene Data Hub Any ...

  18. Interview with Bart Newland, Addgene’s Newest Board Member

    Type
    Blog Post
    ...large companies, Genzyme and Biogen. They’re very different jobs. Being general counsel at a small start-up...companies. It's one of those "every day is something different" type of jobs, which really suits my personality... Jennifer Tsang...

  19. AAV Packaged on Request is Here!

    Type
    Blog Post
    ...repository to be packaged in your choice of five different serotypes.   We are so excited for all the ways...Expanding our viral vector service Addgene has offered in-stock viral vectors since 2016, with the goal... Packaged on Request banner.   We currently offer serotypes AAV1, AAV2, AVV5, AAV8, or AAV Retrograde...

  20. Bringing Sustainable Practices to the Lab: Easy Action Items

    Type
    Blog Post
    ...sustainability problem, how can one little change make a difference? Individual changes to our behavior can lead ...energy, so even one less run a week makes a big difference. Clean out freezers so you can make room and ...only better for the environment, but most likely safer for researchers, as well. Additional resources...
Showing: 681 - 700 of 1052 results