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  2. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...diseases in which sequence replacement holds some kind of fitness advantage but error-prone repair of the... the gene would be disastrous (i.e. if breaking the gene is worse than leaving the mutation alone). CRISPR...

  3. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    ...Photons Chemical reaction High-energy photons Kinetics of Photon Generation Slower Faster Cofactors...been identified in various species with differing kinetics, substrate requirements and photon emission wavelengths...

  4. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ... those first. Depending on the markers you are looking at and the antibodies you are using, you may need... general consensus seems to be that if you are looking for a rare population or are unsure what amount...

  5. Clear and Accessible Writing

    Type
    Blog Post
    ...Addgene was built on the concept of accessibility: making plasmids more accessible to scientists around the...helpfully. You and your readers are now a team, working together to share information. Sometimes it is ...

  6. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...useless without an adjacent PAM or protospacer-flanking sequence (PFS) in some cases. The resource displays...they're available through Addgene. So whether you're looking for the exact Cas9 plasmid used by Jinek et al....

  7. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...performed to amplify the target using PCR. Instead of tracking the reaction progress as in real time PCR, each...introduced how digital PCR works, let’s explain what kind of assays can be used to sniff out your desired ...

  8. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ... and careful Addgenies, but also a lot of good tracking. Our Software and IT Teams made major upgrades...plasmids in the repository. The Outreach Team has been making sure scientists know about the new initiative and...

  9. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...FRET-based sensors such as Twitch. While calcium is king in the sensor world, there are sensors available...expression and downstream monitoring. Nervous about making a virus for your biosensor? Don’t be! We have you...

  10. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...replicate and spread. Instead, viruses replicate by hijacking the host’s system to produce more virus and continue...viral applications to help you start (safely!) thinking about the possibilities. Ready to plan your next...

  12. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...information on this system! GPCR-APEX: Interested in tracking the downstream signaling pathways mediated by ... JA, Gygi SP, Kruse AC (2017) Multidimensional Tracking of GPCR Signaling via Peroxidase-Catalyzed Proximity...

  17. Plasmids 101: Blue-white Screening

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    Blog Post
    ... blue, indicating that your IPTG and x-gal are working as they should be. Don't rush the process: It...between blue and white colonies. Take care in making your plates: X-gal is light and temperature sensitive...

  18. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...conserved cellular machinery to process gRNAs. Hijacking tRNA processing to make gRNAs In a recently published...Cas toolkit in human cells. Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK. Mol Cell. 2014 May 22...

  19. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...of the same technical advantages that come with working with prokaryotes. This includes but is not limited...selection rather than antibiotic selection when working with yeast, due to high rates of spontaneously ...

  20. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ... cardiovascular and metabolic diseases. We are knocking out genes that have been implicated in disease...about the history. How had investigators done this kind of work before? Musunuru: In the "olden days," not...
Showing: 681 - 700 of 926 results