We narrowed to 937 results for: KIN

1. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

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   ...researchers to utilize and develop these tools by making their own fluorescent protein technologies widely...learn more about Xin and Fatima's work and keep checking back in with the Addgene blog for future award...

2. Plasmids 101: Gram Negative and Positive Bacteria

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   ...and how it can be used in a lab? Here we’ll be talking about the ins and outs of this classification system...negative (red/pink) staining. Image credit: Mark Perkins https://www.flickr.com/photos/occbio/7092385559...

3. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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   ...effectiveness of CRISPR/Cas9, scientists are still looking for ways to diminish the possibility of those off-target...s Disease.” Journal of Alzheimer's disease & Parkinsonism. https://doi.org/10.4172/2161-0460.1000439 6...

4. Predicting Adverse Reactions to Monoclonal Antibody Drugs

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   ...differentiated into dentritic cells or macrophages through cytokine induction (Groell et.al, 2018). Thus, microscopic...mimic the human lymphatic system or even the human skin and subcutaneous models (Groell et.al, 2018). Treating...

5. Trading Spaces - Visiting Scientist Edition

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   ...you have one) to communicate your plan as well. Making logistical and funding arrangements Visiting scientist...really good time! If it sounds right for you, start thinking outside of your current research box, send out...

6. The MTA at Addgene

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   ...centralized tracking when biological reagents are shared — both at what time and with whom. Tracking these ...

7. Multiplexed Overexpression of Regulatory Factors (MORF) collection

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   ...valuable reference if you are looking to understand a pathway, or just looking for the best astrocyte differentiation...

8. Early Career Researcher Toolbox: Free Tools for Science Writing

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   .... There’s also tips for setting writing goals, tracking progress, and starting a writing group. Loop Habit...literally a tomato! Image from Gerlos.   Tools for checking grammar and jargon Hemingway Editor  This is a...

9. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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   Blog Post

   ...diseases in which sequence replacement holds some kind of fitness advantage but error-prone repair of the... the gene would be disastrous (i.e. if breaking the gene is worse than leaving the mutation alone). CRISPR...

10. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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    ...initiates the extracellular signaling-regulated kinase (ERK) signaling cascade. This signaling is a lot...signaling by using a fluorescent reporter for Erk kinase translocation. These results demonstrate that OptoNBs...

11. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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    ...efficiency. Check out Addgene’s blog to learn about making your own competent cells and our protocols page...passed down through cell division. You can find all kinds of different lentiviral and AAV plasmids as well...

12. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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    ...however can interfere with other genetic pathways making it difficult to link a phenotype to the gene being...TurboID and miniTurbo Article contributed by Eric Perkins  Listen to the TurboID and miniTurbo podcast segment...

13. Screening for Successful Genome Editing with Digital PCR

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    ...performed to amplify the target using PCR. Instead of tracking the reaction progress as in real time PCR, each...introduced how digital PCR works, let’s explain what kind of assays can be used to sniff out your desired ...

14. Addgene’s Viral Service - Why Virus? Why Now?

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    ... and careful Addgenies, but also a lot of good tracking. Our Software and IT Teams made major upgrades...plasmids in the repository. The Outreach Team has been making sure scientists know about the new initiative and...

15. Clear and Accessible Writing

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    ...Addgene was built on the concept of accessibility: making plasmids more accessible to scientists around the...helpfully. You and your readers are now a team, working together to share information. Sometimes it is ...

16. CasPEDIA: A Functional Classification of Cas Enzymes

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    ...useless without an adjacent PAM or protospacer-flanking sequence (PFS) in some cases. The resource displays...they're available through Addgene. So whether you're looking for the exact Cas9 plasmid used by Jinek et al....

17. Viral Vectors 101: Inverted Terminal Repeats

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    ...proteins and mutations in the trs site reduce Rep nicking. Since Rep proteins are important for viral genome...PMID: 28967288. Shitik, E. M., Shalik, I. K., & Yudkin, D. V. (2023). AAV- based vector improvements unrelated...

18. Viral Vectors 101: Biosensors

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    ...FRET-based sensors such as Twitch. While calcium is king in the sensor world, there are sensors available...expression and downstream monitoring. Nervous about making a virus for your biosensor? Don’t be! We have you...

19. OCaMP: A New Calcium Indicator for Neural Imaging

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    ...box above. The number of action potential (AP) spiking events are indicated below the trace; asterisk ... now available through Addgene. Whether you're working with cell cultures, slice preparations, or in vivo...

20. Fluorescent Proteins 101: Luciferases

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    ... light Chemical reaction High-energy photons Kinetics of photon generation Slower Faster Cofactors...and more. These variant proteins offer different kinetics, substrate requirements, and photon emission wavelengths...