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We narrowed to 750 results for: mpo

Showing: 681 - 700 of 750 results

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Experimenting in an Open Source lab: from CRISPR to Cats

Type

Blog Post

...belong to. The team at the Victoria Makerspace is composed of computer scientists, engineers, and biologists...
Tips to Make the Most of a Scientific Conference

Type

Blog Post

...also have a dedicated app with a social media component, but if it doesn’t seem like this is being used...
Tips for a 1st time AAV user (by a Rookie AAV user)

Type

Blog Post

...virus from the cell pellet alone. If yield is important, or if the viral particles of the AAV serotype...
10 Basic tips for mammalian cell culture

Type

Blog Post

...passage are too young and should not be used for important transfection experiments right after they’ve been...
xCas9: Engineering a CRISPR Variant with PAM Flexibility

Type

Blog Post

...improvement in off-target:on-target editing ratio. Importantly, the observed off-target loci displayed a broad...
Polymerase Chain Reaction Overview and Applications

Type

Blog Post

...multiple starting DNA templates in one sample. It is important to note however, that multiplex PCR is more complicated...
Viral Vectors 101: Producing Your rAAV

Type

Blog Post

...or producer cells for further processing. What component(s) you need to collect will depend on the serotype...
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

Type

Blog Post

...pegRNAs for each desired edit, it simply becomes impossible (or at least unreasonable) to test them all. ...
AAV Vector Quality Control: Going the Extra Mile with NGS

Type

Blog Post

...production process? These questions are especially important when producing multiple vectors in parallel because...
Cloning Mammalian Cells with the Agarose Method

Type

Blog Post

...antibiotics. Lastly, and perhaps obviously, it is important to have a functional assay in place to screen ...
3D Printing Meets CRISPR Cas9

Type

Blog Post

...function in the human body?” … and probably most importantly… "how do we know this?” Over the past fifteen...
Fluorescent Tagging of Endogenous Genes with SapTrap

Type

Blog Post

... mitosis, mitochondrial fission/fusion, nuclear import, and neuronal trafficking. Although FPs have enabled...
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

Type

Blog Post

...protein capsid encapsulating the genome is majorly composed of three proteins that can be easily modified ...
Google Forums Round Up: First Impressions of NgAgo

Type

Blog Post

...respondents (transfection method, amount of each component per transfection etc.). Take Pooran's NgAgo Survey...
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

Type

Blog Post

...native gels fail to detect EGFP dimerization. Importantly, all of these assays are performed in vitro. ...
Light Sheet Fluorescence Microscopy

Type

Blog Post

...addition, the use of viruses allows much better spatiotemporal control over labeling methods as compared to...
Plasmids 101: Multicistronic Vectors

Type

Blog Post

...expression levels between two or more genes is important. How do multicistronic vectors work? Scientists...
Tips for Using BLAST to Verify Plasmids

Type

Blog Post

...Nucleotide collection (nr/nt)” as it contains a composite of GenBank, EMBL, DDBJ, and PDB sequences and ...
Which Fluorescent Protein Should I Use?

Type

Blog Post

...temperature). pH Stability: This parameter is important if you are planning to express the FP in acidic...
Isolating Genomic Regions of Interest with the CRISPR System

Type

Blog Post

...this technique, researchers have begun to make important discoveries in various biological fields. In 2015...

Showing: 681 - 700 of 750 results

Results per page:

Experimenting in an Open Source lab: from CRISPR to Cats

Tips to Make the Most of a Scientific Conference

Tips for a 1st time AAV user (by a Rookie AAV user)

10 Basic tips for mammalian cell culture

xCas9: Engineering a CRISPR Variant with PAM Flexibility

Polymerase Chain Reaction Overview and Applications

Viral Vectors 101: Producing Your rAAV

PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

AAV Vector Quality Control: Going the Extra Mile with NGS

Cloning Mammalian Cells with the Agarose Method

3D Printing Meets CRISPR Cas9

Fluorescent Tagging of Endogenous Genes with SapTrap

Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

Google Forums Round Up: First Impressions of NgAgo

When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

Light Sheet Fluorescence Microscopy

Plasmids 101: Multicistronic Vectors

Tips for Using BLAST to Verify Plasmids

Which Fluorescent Protein Should I Use?

Isolating Genomic Regions of Interest with the CRISPR System

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