We narrowed to 760 results for: mpo

1. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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   Blog Post

   ...pegRNAs for each desired edit, it simply becomes impossible (or at least unreasonable) to test them all. ...

2. Polymerase Chain Reaction Overview and Applications

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   Blog Post

   ...multiple starting DNA templates in one sample. It is important to note however, that multiplex PCR is more complicated...

3. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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   ...protein capsid encapsulating the genome is majorly composed of three proteins that can be easily modified ...

4. Tips for a 1st time AAV user (by a Rookie AAV user)

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   Blog Post

   ...virus from the cell pellet alone. If yield is important, or if the viral particles of the AAV serotype...

5. 10 Basic tips for mammalian cell culture

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   ...passage are too young and should not be used for important transfection experiments right after they’ve been...

6. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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   Blog Post

   ...improvement in off-target:on-target editing ratio. Importantly, the observed off-target loci displayed a broad...

7. 3D Printing Meets CRISPR Cas9

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   Blog Post

   ...function in the human body?” … and probably most importantly… "how do we know this?” Over the past fifteen...

8. Fluorescent Tagging of Endogenous Genes with SapTrap

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   Blog Post

   ... mitosis, mitochondrial fission/fusion, nuclear import, and neuronal trafficking. Although FPs have enabled...

9. AAV Vector Quality Control: Going the Extra Mile with NGS

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   Blog Post

   ...production process? These questions are especially important when producing multiple vectors in parallel because...

10. Cloning Mammalian Cells with the Agarose Method

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    Blog Post

    ...antibiotics. Lastly, and perhaps obviously, it is important to have a functional assay in place to screen ...

11. Google Forums Round Up: First Impressions of NgAgo

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    Blog Post

    ...respondents (transfection method, amount of each component per transfection etc.). Take Pooran's NgAgo Survey...

12. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    Blog Post

    ...native gels fail to detect EGFP dimerization. Importantly, all of these assays are performed in vitro. ...

13. Light Sheet Fluorescence Microscopy

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    Blog Post

    ...addition, the use of viruses allows much better spatiotemporal control over labeling methods as compared to...

14. Plasmids 101: Multicistronic Vectors

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    Blog Post

    ...expression levels between two or more genes is important. How do multicistronic vectors work? Scientists...

15. Tips for Using BLAST to Verify Plasmids

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    Blog Post

    ...Nucleotide collection (nr/nt)” as it contains a composite of GenBank, EMBL, DDBJ, and PDB sequences and ...

16. Which Fluorescent Protein Should I Use?

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    Blog Post

    ...temperature). pH Stability: This parameter is important if you are planning to express the FP in acidic...

17. Lentiviral Vector Guide

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    Guide

    ...laboratory, the non-required components have been removed, and the required components have been separated into...our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and common...require different isoforms of these packaging components. Therefore, lentivirus may not be efficiently...) necessary for integration. Not all of these components are necessary for lentiviral production in the...safety. For a summary of all lentiviral plasmid components, see the Lentiviral Plasmid Elements table . ...further replicate on their own. While the viral components are still separated on different plasmids, the...vectors separate transfer, envelope, and packaging components of the virus between different plasmids. The ...

18. Isolating Genomic Regions of Interest with the CRISPR System

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    Blog Post

    ...this technique, researchers have begun to make important discoveries in various biological fields. In 2015...

19. Great Results Start with Great Standard Curves

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    Blog Post

    ... or very similar buffers if not, since buffer components can affect the final readout. When preparing ...

20. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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    Blog Post

    ...purification protocol uses phenol, then it is important to confirm that your sample’s absorption peak ...