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Showing: 681 - 700 of 706 results

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CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

Type

Blog Post

...that the desired edit will be performed on all relevant bases within the editing window, for example all...
Antibodies 101: Immunoprecipitation

Type

Blog Post

...conformations and interactions. For difficult to release proteins, including those in the nucleus, more ...
Viral Vectors 101: Pseudotyping

Type

Blog Post

...Gutierrez-Guerrero, et al, 2020). Because retrovirus is closely related to lentivirus, many retroviral envelope proteins...
Tips for Using FRET in Your Experiments

Type

Blog Post

...FRET occurs. By photobleaching the acceptor, you release the donor's quenching and the fluorescence of the...
Plasmids 101: The Promoter Region – Let's Go!

Type

Blog Post

...cells and typically only in the same or closely related species from which they were derived. Similarly...
New Optogenetic Tools for Cytoskeleton and Membrane Control

Type

Blog Post

...roles played by microtubules, Opto-katanin will surely be useful for a wide variety of experiments. Opto-RhoGEFs...
CRISPR 101: Homology Directed Repair

Type

Blog Post

...double-strand break repair: how to fix a broken relationship. Pardo B, et al. Cell Mol Life Sci (2009). PubMed...
Using Phosphoserine to Study Protein Phosphorylation

Type

Blog Post

...(7). This modification allowed the deletion of release factor 1, the protein responsible for terminating...
Replacing Paper: Tips for Choosing an Electronic lab Notebook

Type

Blog Post

...we have to admit that paper-based record keeping barely copes with the growing amounts of digital data ...
Transferable Skills Guide: Time Management

Type

Blog Post

...as I progress. I’ve also recently started using Trello to manage larger, multi-person projects. Day-to-day...
An Integrin Antibody Toolkit from IPI

Type

Blog Post

...courses on technologies and laboratory techniques related to antibody discovery. You can find cell surface...
CRISPR 101: Validating Your Genome Edit

Type

Blog Post

...outlined below, the technique can be used as a preliminary validation on your bulk population. This way ...
Transferable Skills Guide: Conflict Resolution

Type

Blog Post

...between two different teams at work, I don’t just rely on what I know about those particular teams at that...
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

Type

Blog Post

...observed in a given area. In 2013, Dawen Cai, et al. released a refinement of the Brainbow technology, Brainbow...
Antibodies 101: Multiplex Immunofluorescence

Type

Blog Post

... colocalization factor to assess the spatial relationship between the targets. Or, it might be sufficient...
Plasmids 101: Dimers and Multimers

Type

Blog Post

...overlap with the band corresponding to the nicked or relaxed conformation of the plasmid monomer (see this article...
Recombinase-based State Machines Enable Order-dependent Logic in vivo

Type

Blog Post

...successful implementation of GRSMs depends on the reliability of the parts used to build them. We recommend...
Technique: Probe Phage Genomes for Host Binding Proteins

Type

Blog Post

...membranes will then be exposed to a lysis buffer to release recombinant proteins directly onto the nitrocellulose...
Plasmids 101: Using Transposons in the Lab

Type

Blog Post

...first copy themselves as RNA transcripts and then rely on a reverse transcriptase enzyme to reverse transcribe...
Mycoplasma Contamination: Where Does It Come From and How to Prevent It

Type

Blog Post

...or universal. This method is quick, efficient, reliable and cost-effective (Sung, 2006). The sensor cell-based...

Showing: 681 - 700 of 706 results

Results per page:

CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

Antibodies 101: Immunoprecipitation

Viral Vectors 101: Pseudotyping

Tips for Using FRET in Your Experiments

Plasmids 101: The Promoter Region – Let's Go!

New Optogenetic Tools for Cytoskeleton and Membrane Control

CRISPR 101: Homology Directed Repair

Using Phosphoserine to Study Protein Phosphorylation

Replacing Paper: Tips for Choosing an Electronic lab Notebook

Transferable Skills Guide: Time Management

An Integrin Antibody Toolkit from IPI

CRISPR 101: Validating Your Genome Edit

Transferable Skills Guide: Conflict Resolution

Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

Antibodies 101: Multiplex Immunofluorescence

Plasmids 101: Dimers and Multimers

Recombinase-based State Machines Enable Order-dependent Logic in vivo

Technique: Probe Phage Genomes for Host Binding Proteins

Plasmids 101: Using Transposons in the Lab

Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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