We narrowed to 717 results for: CaS

1. Evolution of Lab Techniques

   Type

   Blog Post

   ...detection methods are also commonly used. In all cases, the result can be quantified by the strength of...

2. How to Negotiate a Successful Lab Start Up Budget

   Type

   Blog Post

   ...that means saying no to a negotiation or, in this case, to academia altogether.   The primary point of ...

3. Is this the right place for me? 8 tactics for choosing a lab

   Type

   Blog Post

   ...undermining personal integrity, destructive innuendo and sarcasm, persistent teasing, name calling, insults, intimidation...

4. Technique: Probe Phage Genomes for Host Binding Proteins

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   Blog Post

   ...what her cat is thinking. Find her on Twitter @JessicaSacher.   References 1. Nobrega, Franklin L., et al...

5. When Fidelity Matters: A frank discussion about ligase fidelity

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   Blog Post

   ...lacking gaps or mismatched base pairs (as in the case for LCR, LDR and Padlock Probes), use of a high ...

6. RNA Interference in Plant Biology: New Tools for an Old Favorite

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   Blog Post

   ...Bassik MC (2016) Systematic comparison of CRISPR/Cas9 and RNAi screens for essential genes. Nat Biotechnol...

7. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

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   Blog Post

   ...of AAV vector we produce containing a DIO/FLEx cassette using next-generation sequencing (NGS) (Guerin...

8. A Guide to Getting Started in Undergrad Research

   Type

   Blog Post

   ...think reflecting on why it is we enjoy, or in some cases, don’t enjoy, is worthwhile and can help us make...

9. Cloning

   Type

   Guide

   ...promoters and into backbones with different selection cassettes). Although gateway cloning is a simple and efficient...

10. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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    Blog Post

    ...other technologies such as optogenetics and CRISPR/Cas9 will help immunologists create even better tools...

11. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

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    Blog Post

    ...and Wei‐Lih Lee. "Photoactivatable GFP tagging cassettes for protein‐tracking studies in the budding yeast...

12. Cancer and the Immune System: Deciphering the Relationship

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    Blog Post

    ... (VEGF) in tumors is attributed to a signaling cascade, where lack of oxygen in the tumor, activates hypoxia...

13. Special Delivery: Fluorophore Targeting for FRET Studies

    Type

    Blog Post

    ...acceptor fluorophores, it’s best to consider these cases separately when talking about the tools that are...

14. A Primer on Optogenetics: Introduction and Opsin Delivery

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    Blog Post

    ...titer must be determined empirically (this is the case for any viral injection into the brain). As a general...

15. Selecting Your Plasmid Purification Kit

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    Blog Post

    ...step and provide troubleshooting advice. In these cases, it can be helpful to select a kit with easy-to-...

16. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type

    Blog Post

    ...range of possibilities in clinical situations. In cases where the objective is to fix a disease-inducing...

17. Troubleshooting and Optimizing a Western Blot

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    Blog Post

    ...concentration to detect low levels of proteins. In this case, it might be faster to run the same amount of protein...