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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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  1. Sequencing Primers

    Type
    Guide
    ..., reverse primer pBluescriptKS TCGAGGTCGACGGTATC For pBluescript vector pBluescriptSK TCTAGAACTAGTGGATC...TCTAGAACTAGTGGATC For pBluescript vector pBMN 5' GCTTGGATACACGCCGC MMLV sequence, for inserts in pBMN retroviral vector...

  2. Addgene's eBook Collection

    Type
    Guide
    ...Overlay Select Fluorescent Proteins 101 August 2017 | First Edition About Fluorescent Proteins 101 ... this eBook, you’ll find tips for choosing a fluorescent protein; information on FRET, biosensors, and...

  3. Promoters

    Type
    Guide
    ...proximal promoter, and distal promoter. Below describes the specifics of these regions in eukaryotic cells...Promoters Used in Research Promoter Expression Description CMV Constitutive Strong mammalian promoter from...Promoters used in Research Promoter Expression Description T7 Constitutive but requires T7 RNA polymerase...

  5. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...lentiviral packaging methods . The three plasmids described above (envelope, packaging, and transfer) are ...resistance is not the only type of selectable marker. Fluorescent reporters, such as GFP, are another common selection...marker. Instead of selection by an antibiotic, fluorescence-activated cell sorting (FACS) is used to sort...

  6. Plan Your Experiment

    Type
    Guide
    ...validate your genetic edit. Figure 1: Flow chart describing the general framework of a CRISPR experiment.... mRNA and Proteins In addition to the methods described above, you can also directly deliver mature mRNA...endosomal approach. Similar to the mRNA method described above, this method is also transient, as the RNPs...

  7. Lentiviral Vector Guide

    Type
    Guide
    ...resistance is not the only type of selectable marker. Fluorescent reporters, such as GFP, are another common selection...marker. Instead of selection by an antibiotic, fluorescence-activated cell sorting (FACS) is used to sort..., M., Sutherland, H., Saenz, D., Bickmore, W., Poeschla, E., & Bushman, F. D. (2007). Role of PSIP1/LEDGF...

  8. Educational Resources

    Type
    Guide
    ...include: Plasmids 101 CRISPR 101 Viral Vectors 101 Fluorescent Proteins 101 Science Career Guide Videos Watch...techniques. Topics include: Genome Engineering Fluorescent Proteins CRISPR Viral Vectors Plasmid Technology...

  9. Science Guides

    Type
    Guide
    ...Collection of guides that describe how popular scientific techniques work...

  10. Cloning

    Type
    Guide
    ...been made with different promoters, tags and fluorescent proteins. Thus, you can clone your gene of interest...
Showing: 701 - 711 of 711 results