We narrowed to 1,052 results for: FER
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TypeBlog Post... been difficult to compare the performance of different nucleases. Nicola Patron’s lab compared the efficiency...genome editing toolkit New Cas protein identified Jennifer Doudna’s lab recently identified Cas14, a family... Jennifer Tsang...
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MassBioEd Career Exploration Day at Addgene
TypeBlog Post...learn about intellectual property from our Tech Transfer team members Tony Cova and Chiara DeNatale and...of the students were interested to see so many different career options in science, with Abigail Anderson...professor. There are amazing opportunities that are offered with a biology major.” After hearing about many... -
Your Top Requested Plasmid in 2015!
TypeBlog Post... name suggests, lentiCRISPR v2 is a lentiviral transfer vector used to deliver both Cas9 and your gRNA...metalloproteinase-9 secretion, and identifying lysine methyltransferase substrates. We’re incredibly happy that lentiCRISPR... forward to another great year of sharing! References 1. Shalem, Ophir, et al. "Genome-scale CRISPR-Cas9... -
Inntags: Innovative Protein Epitope Tagging
TypeBlog Post...antibody. There is one major caveat - a tag may interfere with protein localization and/or function, so ... like FLAG, HA, and Myc can adopt a number of different conformations and thus potentially interact with...our older epitope tags - only time will tell! References 1. Georgieva, Maya V., et al. "Inntags: small... -
More Data for You: Find Articles Citing Addgene Plasmids
TypeBlog Post...validated in. If a plasmid's Addgene ID # has been referenced in other publications, you'll find a link to ...Additional features Once you've clicked on the "# References" link under the "Resource Information" heading...Addgene’s Plasmids 101 eBook Protocols and Plasmid References @Addgene ... -
Microbes: Look, Listen, and Tell
TypeBlog Post...When you think about going to a scientific conference, you may think about sitting amongst a sea of chairs...ultrasound dyes because these dyes “collapse” at different ultrasound frequencies. Read more about ‘Using...communication check out these SciComm blog posts. References 1. Bourdeau, Raymond W., et al. "Acoustic reporter... -
Science Communication for Everyone
TypeBlog Post... how our articles have helped them understand different topics of science, especially disease related ...terms like manipulations, anomaly, and so on, differently than scientists would. For example, “manipulate... receive our science career guide Learn about different careers in science communication Resources on... -
Stepping into a Virtual Internship: My Experience at Addgene
TypeBlog Post...internship would be a perfect way of exploring my different interests but also a way of challenging what I...would look like. While working remotely was a different experience, It was helpful to have Addgenies reassure...spoke with amazing people that were a part of the different departments of the company. I asked questions ... -
Six Spooky Science Stories and Halloween at Addgene
TypeBlog Post...nervous system: infected grasshoppers expressed different proteins in their brains compared to uninfected...and geotactic behavior (Biron et al., 2005). References Biron, D. G., et al. "Behavioural manipulation... Jennifer Tsang... -
Phage Directory: From Phage Therapy to a Repository of Phage Information
TypeBlog Post...test. These includes labs with a large number of different phages or with phages targeting organisms closely...prepare phages for clinical use. There are also different regulations from country to country when it comes... Jennifer Tsang... -
IDP and your PI: A Roadmap for Career Planning and Personal Development
TypeBlog Post...then formulate a plan to further develop your transferable skills and reach your career goals. Although...know your strategies in the comments section! References 1. Yearly Planning Meetings: Individualized Development... What Makes a Good Manager Anyway Developing Transferable Skills During Science Training ... -
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog Post...elements. Regulatory DNA function is typically inferred based on correlative biochemical characteristics...Bioengineering from the University of Pennsylvania. References: 1. Lee HJ, Kim E, Kim JS. Targeted chromosomal...Aug 1;289(31):21312-21324. PubMed. More CRISPR Refereces: Browse CRISPR Plasmids CRISPR Information ... -
Three Tips to Organize Your Lab Notebooks in the New Year
TypeBlog Post...post-its to make finding your most used protocols & references easy to find It’s happened to all of us. It’s...consider using color coded binders and tabs (for different experiments or projects) to make it easier to ...small by identifying the protocols, guides, or references that your labmates ask to borrow most frequently... -
What's New in CRISPR - September 2019
TypeBlog Post...active in E. coli and human cells Two years ago, Jennifer Doudna’s lab discovered a new Cas protein, CasX...human cells. Using the structure of CasX at eight different states of assembly from cryo-electron microscopy... Jennifer Tsang... -
SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog Post... to use the same peptide tag for all sorts of different protein systems and they shouldn’t get in the ...marker expression level. Labs have been trying different approaches to improve CTC capture, but I hope ...Addgene. I am a big fan; thanks for the T-shirt! References: SpyLigase peptide-peptide ligation polymerizes... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog Post...per experiment. In the McKay lab we study how differential access to transcriptional regulatory elements...nuclei extraction. They report that this change offers a significant improvement and simplification of...cell or tissue preparation. All in all, CUT&RUN offers a great alternative to ChIP-seq, especially in ... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog Post...in decreased efficiency of recombination and preferentially leads to gene constructs where the stop cassette...likely generalizable to other systems as well. References: del Valle Rodríguez A, Didiano D, Desplan C...plasmids at Addgene Read our Molecular Biology Reference ... -
Bringing Sustainable Practices to the Lab: Recycling
TypeBlog Post...meaningful changes in the lab could make a big difference in our global impact as scientists. Many scientists... single-stream or city recycling services don’t offer plastic recycling, specialized companies can recycle...Sustainable Practices to the Lab: Easy Action Items References Geyer, Roland, Jenna R. Jambeck, and Kara Lavender... -
An Addgene Co-op: The Intersection of Fun, Friendship, and Work
TypeBlog Post.... Northeastern compiles a list of hundreds of different jobs for students to apply to, and after a grueling...marketing and social media co-op. There are a lot of different worries that come along with entering the workforce...of my co-op. I had to do research on loads of different best practices, create an email strategy, and ... -
Embracing Serendipity: A Crucial Element in the PhD Journey
TypeBlog Post...room for serendipity during your PhD studies and offer two simple steps to welcome these unforeseen moments...project's potential. Widening your search creates fertile ground for serendipitous encounters that could ...enrich the depth and creativity of your work, while offering mental health benefits by encouraging activities...