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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 937 results for: KIN

Showing: 701 - 720 of 937 results

  1. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ... those first. Depending on the markers you are looking at and the antibodies you are using, you may need... general consensus seems to be that if you are looking for a rare population or are unsure what amount...

  2. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...replicate and spread. Instead, viruses replicate by hijacking the host’s system to produce more virus and continue...viral applications to help you start (safely!) thinking about the possibilities. Ready to plan your next...

  3. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...information on this system! GPCR-APEX: Interested in tracking the downstream signaling pathways mediated by ... JA, Gygi SP, Kruse AC (2017) Multidimensional Tracking of GPCR Signaling via Peroxidase-Catalyzed Proximity...

  6. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ... blue, indicating that your IPTG and x-gal are working as they should be. Don't rush the process: It...between blue and white colonies. Take care in making your plates: X-gal is light and temperature sensitive...

  7. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...conserved cellular machinery to process gRNAs. Hijacking tRNA processing to make gRNAs In a recently published...Cas toolkit in human cells. Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK. Mol Cell. 2014 May 22...

  8. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...of the same technical advantages that come with working with prokaryotes. This includes but is not limited...selection rather than antibiotic selection when working with yeast, due to high rates of spontaneously ...

  9. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ... cardiovascular and metabolic diseases. We are knocking out genes that have been implicated in disease...about the history. How had investigators done this kind of work before? Musunuru: In the "olden days," not...

  11. Summer SciComm: Preprints

    Type
    Blog Post
    ...unpublished results at a conference? Post your working manuscript as a preprint too and you can share ...American Society for Cell Biology in a position working with preprints in life sciences. He also posted...

  12. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...proteins in your assay.  Narrowing the field After working your way through the above steps, you may find ...an antibody website. In 2016, the International Working Group for Antibody Validation proposed 5 pillars...

  13. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...developed by engineering the Cas12f scaffold gRNA and making strategic mutations in the DNA binding cavity of...half the size of the Cas9 or Cas12a based systems, making it well suited for AAV packaging. Most importantly...

  15. Addgene: The First Twenty Years

    Type
    Blog Post
    ...people knew how to access Addgene, and that we were making it possible for scientists to both deposit and ...through physical outreach, visiting organizations, knocking on doors, and attending conferences. And when ...
Showing: 701 - 720 of 937 results