We narrowed to 933 results for: KIN

... blue, indicating that your IPTG and x-gal are working as they should be. Don't rush the process: It...between blue and white colonies. Take care in making your plates: X-gal is light and temperature sensitive...

...conserved cellular machinery to process gRNAs. Hijacking tRNA processing to make gRNAs In a recently published...Cas toolkit in human cells. Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK. Mol Cell. 2014 May 22...

...of the same technical advantages that come with working with prokaryotes. This includes but is not limited...selection rather than antibiotic selection when working with yeast, due to high rates of spontaneously ...

... cardiovascular and metabolic diseases. We are knocking out genes that have been implicated in disease...about the history. How had investigators done this kind of work before? Musunuru: In the "olden days," not...

...unpublished results at a conference? Post your working manuscript as a preprint too and you can share ...American Society for Cell Biology in a position working with preprints in life sciences. He also posted...

...proteins in your assay.  Narrowing the field After working your way through the above steps, you may find ...an antibody website. In 2016, the International Working Group for Antibody Validation proposed 5 pillars...

...developed by engineering the Cas12f scaffold gRNA and making strategic mutations in the DNA binding cavity of...half the size of the Cas9 or Cas12a based systems, making it well suited for AAV packaging. Most importantly...

...colleagues are working on developing new surface display vectors to improve expression and trafficking of these...

...people knew how to access Addgene, and that we were making it possible for scientists to both deposit and ...through physical outreach, visiting organizations, knocking on doors, and attending conferences. And when ...

... STED nanoscopy Article contributed by Eric J. Perkins Listen to Endosomal pH Sensors podcast segment ...approach allows minute-by-minute analysis of the kinetics of chromatin regulation and subsequent mathematical...

...out there, it’s obvious that CRISPR is a ground-breaking technology that’s already had a huge impact on...nuclease itself. Design and testing of eSpCas9 Looking at the structure of the Cas9 nuclease (PDB ID: ...

...something that is actually useful to you. When embarking on a new literature search, try to find a recent... on in any given research area. Unless you are looking for a particular protocol or are trying to replicate...

... to own the process entirely remains a large undertaking. Priority number one was to ensure that this ... load it on to our new MiSeq and set up the run making sure to note the appropriate barcode for each sample...

... ability to study and understand the complex interworking of the 86 billion neurons in the human brain... opens the door to in vivo experiments such as tracking the synaptic maturation pattern of hippocampal...

... been working with the Rare Genomics Institute to provide plasmid grants to researchers working on rare...

...or their translation disrupted through physical blocking of the translation machinery. In the Sánchez lab...planarians. Depending on which planarian species one is working with, the protocol will slightly differ. For example...

...fluorescent protein Dronpa. The authors tracked protein kinase A (PKA) activity at the plasma membrane in living...artifacts, it has better folding and maturation kinetics than other dark fluorescent proteins, it has good...

...specific gene or brain region. When it comes to making the genetic manipulation, there are two main decisions...should be used? Modifying gene expression Generally speaking, one may want to achieve either inhibition or ...

...engineered mice: transgenic mice, knockin and knockout mice, and conditional knockin or knockout mice. While these...

...detection We and other members of the lab had been working on advancing the paper-based platform toward a ...when we needed additional expertise. Perhaps most striking is that there were seven institutions involved...