We narrowed to 934 results for: Kin

1. CasPEDIA: A Functional Classification of Cas Enzymes

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   ...useless without an adjacent PAM or protospacer-flanking sequence (PFS) in some cases. The resource displays...they're available through Addgene. So whether you're looking for the exact Cas9 plasmid used by Jinek et al....

2. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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   ...initiates the extracellular signaling-regulated kinase (ERK) signaling cascade. This signaling is a lot...signaling by using a fluorescent reporter for Erk kinase translocation. These results demonstrate that OptoNBs...

3. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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   ...behind. While commercial kits are available for making small point mutations, large deletions/insertions...-12 kb range. REPLACR-mutagenesis also requires making your own electrocompetent cells in which the phage...

4. Plasmids 101: Blue-white Screening

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   ... blue, indicating that your IPTG and x-gal are working as they should be. Don't rush the process: It...between blue and white colonies. Take care in making your plates: X-gal is light and temperature sensitive...

5. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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   ...conserved cellular machinery to process gRNAs. Hijacking tRNA processing to make gRNAs In a recently published...Cas toolkit in human cells. Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK. Mol Cell. 2014 May 22...

6. Plasmids 101: Yeast Vectors

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   ...of the same technical advantages that come with working with prokaryotes. This includes but is not limited...selection rather than antibiotic selection when working with yeast, due to high rates of spontaneously ...

7. Kiran Musunuru on the Newest TALEN Genome-Editing System

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   ... cardiovascular and metabolic diseases. We are knocking out genes that have been implicated in disease...about the history. How had investigators done this kind of work before? Musunuru: In the "olden days," not...

8. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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   ... STED nanoscopy Article contributed by Eric J. Perkins Listen to Endosomal pH Sensors podcast segment ...approach allows minute-by-minute analysis of the kinetics of chromatin regulation and subsequent mathematical...

9. Addgene: The First Twenty Years

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   ...people knew how to access Addgene, and that we were making it possible for scientists to both deposit and ...through physical outreach, visiting organizations, knocking on doors, and attending conferences. And when ...

10. Antibodies 101: Selecting the Right Antibody

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    ...proteins in your assay.  Narrowing the field After working your way through the above steps, you may find ...an antibody website. In 2016, the International Working Group for Antibody Validation proposed 5 pillars...

11. What's New in CRISPR - November 2021

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    ...developed by engineering the Cas12f scaffold gRNA and making strategic mutations in the DNA binding cavity of...half the size of the Cas9 or Cas12a based systems, making it well suited for AAV packaging. Most importantly...

12. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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    ...colleagues are working on developing new surface display vectors to improve expression and trafficking of these...

13. Summer SciComm: Preprints

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    ...unpublished results at a conference? Post your working manuscript as a preprint too and you can share ...American Society for Cell Biology in a position working with preprints in life sciences. He also posted...

14. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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    ...out there, it’s obvious that CRISPR is a ground-breaking technology that’s already had a huge impact on...nuclease itself. Design and testing of eSpCas9 Looking at the structure of the Cas9 nuclease (PDB ID: ...

15. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

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    ...something that is actually useful to you. When embarking on a new literature search, try to find a recent... on in any given research area. Unless you are looking for a particular protocol or are trying to replicate...

16. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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    ... been working with the Rare Genomics Institute to provide plasmid grants to researchers working on rare...

17. Educational Planarian Tools for Teaching Developmental Biology Techniques

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    ...or their translation disrupted through physical blocking of the translation machinery. In the Sánchez lab...planarians. Depending on which planarian species one is working with, the protocol will slightly differ. For example...

18. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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    ...fluorescent protein Dronpa. The authors tracked protein kinase A (PKA) activity at the plasma membrane in living...artifacts, it has better folding and maturation kinetics than other dark fluorescent proteins, it has good...

19. Mouse Modeling, Part 2: Breeding and Crossing Mice

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    ...engineered mice: transgenic mice, knockin and knockout mice, and conditional knockin or knockout mice. While these...

20. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    ... to own the process entirely remains a large undertaking. Priority number one was to ensure that this ... load it on to our new MiSeq and set up the run making sure to note the appropriate barcode for each sample...