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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 960 results for: REP

Showing: 701 - 720 of 960 results

  1. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ...for PIs to include in their annual NIH progress reports information on the Individual Development Plans...comments were about the astounding lack of reproducibility being recognized in published work even in ...

  2. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    ...have deposited their plasmids into the Addgene repository. More than 4,500 plasmids have already been deposited...aware of what Addgene can do for them and how the repository can help them share their materials. The new ...

  4. Protocol - How to Design Primers

    Type
    Protocol
    ...together. One must selectively block and unblock repeatedly the reactive groups on a nucleotide when adding...

  5. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ... evolution, Tn7-like transposons captured and repurposed nuclease-deficient CRISPR-Cas systems to help...bacterial genome engineering tools, with early reports of 40%–60% efficient integration (Klompe et al....

  6. An Addgene Summer Internship

    Type
    Blog Post
    ...capstone project report and presentation, during the final week at Addgene. This report shared my work ...

  7. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ...scientific research are misidentified! Rigor and reproducibility of scientific data depends on validated reagents.... For instance, recently Cellosaurus received a report that HT-55, which was described as a colon cancer...

  11. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...swapped for alternative genetic cargo, they can’t reproduce more functional viruses later on. These built-... is! Addgene has a number of ready-to-use viral preps available for a variety of broadly applicable and...

  12. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...systems during experiments – neither ideal for the intrepid researcher. There existed, therefore, a need for...disturbed, the brain fibers could not be directly repurposed for gut implantation. The fibers they ended up...

  13. The Strength of Story Telling

    Type
    Blog Post
    ...taking beautiful photographs of their studies, reporting on amazing women in science, or asking someone...yourself open to conversation — put your work on preprint servers, share your email address far and wide...

  14. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...with implanted electrodes. The Archon1 voltage reporter allows a way to monitor neuronal electrical activity...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

  15. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...) using in utero electroporation or with AAVs (prepared with Addgene #104110 - #104112) (Figure 2). We...with an index-optimized clearing agent." Cell reports14.11 (2016): 2718-2732. PubMed PMID: 26972009. Ke...

  16. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...field. Those engineered sensors were designed to replace synthetic calcium dyes, which had been in use since...calcium-binding sites as possible for added sensitivity. As reported in Nature Methods, the FRET changes of these Twitch...

  19. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ... we found in our map have an impact on virus replication. While we and our collaborators are starting ...straight-forward. All constructs are tagged with a 2xStrep-tag for easy detection and affinity purification...

  20. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...Make sure your cells are as healthy as can be to prepare them for the arduous process of generating viral...Resources on Addgene.org Find Ready-to-Use AAV Preps Find Lab Protocols for Work with Virus Watch Our...
Showing: 701 - 720 of 960 results