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Showing: 701 - 711 of 711 results

  1. Immunocytochemistry

    Type
    Protocol
    ...nM DAPI working solution: Prepare a 300 µM DAPI stock solution by diluting 2.1 µL of the 5 mg/mL DAPI ...working solution by diluting 5 µL of the 300 µM DAPI stock solution into 5 mL PBS. Protect from light. Procedure...

  2. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ... heat-inactivated FBS and 5mL of 100X glutaGRO. Store at 4 °C. Pro-Tip Different brands and FBS lots can...and have been sufficiently expanded, prepare cell stocks and/or harvest to test for protein expression. ...

  3. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ... want to clone from the start codon (ATG) to the stop codon (TGA, in this example). Assuming you are ...taking the final 18bases of the ORF, including the stop codon (5'-TGGCATATCTCGAAGTACTGA-3'), then adding...

  4. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ... as follows: In the Chart Editor , select the Customize tab. Select the Series drop down menu. Select ...trendline in Google Sheets as follows: In the Customize tab, select the Label drop down menu and choose...

  5. Weighing Reagents Protocol

    Type
    Protocol
    ...of materials for an experiment or reagents for a stock mixture is an essential laboratory technique, as...

  6. Centrifugation

    Type
    Protocol
    ...unbalanced or improperly loaded centrifuge. Immediately stop the centrifuge and investigate the issue. When you...

  9. Western Blot

    Type
    Protocol
    ...microcentrifuge tube. Use the lysate immediately or store at -80 °C until ready to use. Section 2: Determine...

  11. CRISPR Library Amplification

    Type
    Protocol
    ...in representation compared to the pre-amplified stock. A last option is to perform a gel extraction of...
Showing: 701 - 711 of 711 results