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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 738 results for: Sis;

Showing: 701 - 720 of 738 results

  1. Water Bath Protocol

    Type
    Protocol
    ...allow the temperature to stabilize. Use water resistant markers, such as permanent markers, to label your...water bath, be sure to use a marker that is also resistant to chemicals such as ethanol. Writing on the tops...

  4. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...generate predictions of these outcomes. Rigorous analysis is essential to ensure off-target events are below...

  5. A Control for All Seasons

    Type
    Blog Post
    ... dead or dying cells so that you can base your analysis on living cells only (for example, propidium iodide...

  13. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ... cells (for example, plasmid with a puromycin resistance cassette, such as PX459). After you co-transfect...

  14. DNA Quantification

    Type
    Protocol
    ...such as TE). You will get much more accurate and consistent readings from DNA in a buffered solution. Reference...

  15. Protocol - How to Design Primers

    Type
    Protocol
    ...Reaction Plasmid Cloning by PCR Agarose Gel Electrophoresis Primer Design for PCR Oligonucleotide primers...

  18. Ligation Independent Cloning

    Type
    Protocol
    ...linearized vector from the reaction mixture by gel electrophoresis followed by gel purification . The cut vector...

  20. Pipetting Protocol

    Type
    Protocol
    ...multiple “stops” on the plunger, where you feel resistance while pressing down on the pipette. Stop at the...
Showing: 701 - 720 of 738 results