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We narrowed to 1,052 results for: FER

Showing: 721 - 740 of 1052 results
  1. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

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    Blog Post
    .... Northeastern compiles a list of hundreds of different jobs for students to apply to, and after a grueling...marketing and social media co-op. There are a lot of different worries that come along with entering the workforce...of my co-op. I had to do research on loads of different best practices, create an email strategy, and ...
  2. Bringing Sustainable Practices to the Lab: Recycling

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    Blog Post
    ...meaningful changes in the lab could make a big difference in our global impact as scientists. Many scientists... single-stream or city recycling services don’t offer plastic recycling, specialized companies can recycle...Sustainable Practices to the Lab: Easy Action Items References Geyer, Roland, Jenna R. Jambeck, and Kara Lavender...
  3. Embracing Serendipity: A Crucial Element in the PhD Journey

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    Blog Post
    ...room for serendipity during your PhD studies and offer two simple steps to welcome these unforeseen moments...project's potential. Widening your search creates fertile ground for serendipitous encounters that could ...enrich the depth and creativity of your work, while offering mental health benefits by encouraging activities...
  4. Choosing a Good Mentor for Scientists

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    Blog Post
    ...in this series. Check out Joanne's Reddit AMA Different types of mentors for grad school vs. postdoc Choosing...Choosing an advisor to complete a successful PhD is different from choosing a lab for postdoctoral work. However...think, “Oh, but that won’t happen to me – I am different.”  Unfortunately, a bad mentor can be bad news...
  5. History of CRISPR Cas - A tale of survival and evolution

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    Blog Post
    ...genes) system. This popular genome engineering tool offers flexibility, multiplexibility, and ease of use....moieties carry out these functions using slightly different Cas proteins and PAM sequences. A total of eight...involves CRISPR/Cas technology? Let us know.  References Ishino Y, Hideo, S, Makino K, Mitsuko A, Nakata...
  6. Intro to Our Summer SciComm Series!

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    Blog Post
    ...)    Science communication, or science writing, refers to the process of communicating science to laypeople...knowledge or interest in STEM subjects; we generally refer to them as the “interested public.” Subscribers ...field-specific terminology or language. This can refer both to terms that are only used in the field and...
  7. Meet the New Addgene Board Members!

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    Blog Post
    ...diseases. “Today, I am a scientist that helps with different aspects of manufacturing and quality, especially...microRNA-based system for polymerase II-regulated RNA interference. In the first two months since publication, ... Jennifer Tsang...
  8. Antibodies 101: Epitope Tags

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    Blog Post
    ...Ready to start epitope tagging?   References and Resources References Terpe, K., Overview of tag protein...obvious con of GFP is its size – which can possibly interfere with protein function and localization. If you...
  9. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

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    Blog Post
    ...combinatorial expression of these three genes with different copy numbers, producing dozens of color hues (... using in utero electroporation. Then at your preferred time points, sacrifice the animals and fix the...website: https://dn-en.med.kyushu-u.ac.jp/     References Cai, Dawen, et al. "Improved tools for the Brainbow...
  10. Plasmids 101: TOPO Cloning

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    Blog Post
    ...topoisomerase I (1). If a 5′ hydroxyl group from a different DNA strand comes along, it can attack this covalent...Gel purify your PCR product and incubate it with buffer, Taq and dATPs at 72C for 10-15min.  When mixing...may allow you to see colonies within 8 hours. References: 1. Shuman S. "Recombination mediated by vaccinia...
  11. Hot Plasmids: FRET-Based Biosensors

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    Blog Post
    ...field is a series of fluorescence resonance energy transfer (FRET)-based calcium biosensors. Perhaps most ...enable observation of the interaction between different molecules like zinc and calcium. Ultimately, Merkx... start using it. For us, it’s like a test.” References Thestrup et al. Optimized ratiometric calcium...
  12. Early Career Researcher Toolbox: Free Tools for Science Writing

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    Blog Post
    ...media, etc.). The free version of Grammarly also offers basic writing suggestions, like alternative word...same features, but this chart highlights the differences between these two citation managers. If you want...a favorite writing tool, let us know below! References and Resources You Can Grad School podcast episode...
  13. Creating accessible biology activities in schools with BioBits

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    Blog Post
    ...are, how complicated (and again, expensive) the different machines are, and how many regulations are in ...advocacy. You can email her at [email protected].   References 1. Huang, Ally, et al. "BioBits™ Explorer: A ...lab techniques Check out our molecular biology reference pages Find plasmids from the BioBits BrightTM ...
  14. FPbase: A new community-editable fluorescent protein database

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    Blog Post
    ...sequences and lineages, FRET efficiencies, additional references, etc...) and a more robust database structure...address).  One may also simply attach important references that serve to further characterize a protein ...Currently, all submissions also require a DOI referencing primary literature, and every effort is made ...
  15. Trading Spaces - Visiting Scientist Edition

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    Blog Post
    ...remote collaborations Transferable Skills Guide: External collaborations Transferable Skills Guide: Teamwork...ask questions if you don’t understand something. Offer to help out wherever you can and don’t forget to...
  16. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

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    Blog Post
    ...used a solution of cell culture medium, which is buffered and contains the pH indicator phenol red. We froze...permeable to CO2 at low temperatures. We tested two different types of tubes: the ones we use to store lentivirus...also using the thawed virus within 24 hours. References 1. Murphy BM, Swarts S, Mueller BM, van der Geer...
  17. The Power Behind NGS Plasmid Validation: seqWell

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    Blog Post
    ...hundreds of different plasmids from a single Illumina MiSeq® run. As shown in Fig. 2, below, differences in input...constructs like plasmids. The plexWell™ workflow also offers a highly efficient NGS-based alternative to Sanger...
Showing: 721 - 740 of 1052 results