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We narrowed to 958 results for: Tes

Showing: 721 - 740 of 958 results

  1. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...as KLH or Freund’s adjuvant is injected, which stimulates the overall immune response. Once the initial...weeks to increase the antibody titer. The titer is tested following each immunization until the desired level...

  3. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...load The Wikipedia definition of cognitive load states: “cognitive load refers to the used amount of working...even the most enthusiastic of learners.  What contributes to cognitive load?  Everything! Here are some...

  4. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...and isolated with antibiotic selection. The team tested this system in vitro and in vivo. They identified...fluorescent proteins into one bright particle that segregates into one cell during cell division. To achieve...

  5. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...below): 1. Resistant cells among the separate replicates would contain the same labels - i.e. they would...subpopulation 2. Resistant cells among the separate replicates would have different labels - i.e. they would...

  7. Pipetting Protocol

    Type
    Protocol
    ...video for tips on pipetting in the lab. Equipment Pipettes Pipette tips Waste container Containers to hold...Tips Although there are many different types of pipettes that have different mechanisms to disperse liquids...liquids, this protocol is focused on single channel pipettes. These come in standard sizes: P2, P10, P20, P200...would read as 050. Each volume display on these pipettes will also have small tick marks at the bottom....Liquids of various viscosity have different flow rates. The more viscous a liquid, the lower the flow rate...’s important for scientists to calibrate their pipettes to make sure that they are properly tuned to dispense...

  8. A Look at Addgene's QC Process

    Type
    Blog Post
    ...the deposited sequence(s) in SnapGene. SnapGene annotates the plasmid maps based on SnapGene’s “Common Feature...at a best practices meeting, where the QC team updates and reviews our sequencing QC standards to ensure...

  9. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ... with plasmids that allow them to eat unusual substrates that other organisms can’t use. Other plasmids...undiscovered functions, many are simply genetic parasites. Cryptic plasmids tend to be small and poorly ...

  10. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...transfer plasmid, the component that physically integrates into the host genome, the virus is replication...decontaminating liquid waste, remember that the percentage indicates the proportion of the decontaminate in the waste...

  11. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...clones and thus the potential to bind many different sites on the target protein they tend to have a strong...and bind to your primary antibody. Manning et al. tested a variety of commercially available secondary antibodies...

  12. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...prohibitively expensive for many labs. We’ve created and tested plasmids that use CRISPR/Cas9 to endogenously tag... few single base pair mutations in crRNA target sites found within the homology areas of the plasmid to...

  14. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...immunogenicity of AdVs is what makes them ideal candidates for applications in oncolysis and vaccination...by recombination. It is therefore recommended to test for the presence of RCA in the viral stock by doing...

  17. Tips for Technical Support Calls

    Type
    Blog Post
    ...number of messages in our thread or remove a good 5 minutes from our phone call. If your concern is based...get. There are questions that are answerable in minutes and questions that could take weeks to resolve ...

  18. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...virus in Brazil that was correlated with greater rates of infection and rare, but severe, symptoms, including...unlike most research, but the urgent need for the test did catalyze our resolve to get the work done as...

  19. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...0.22 µm filter) or by a centrifugation step (5 minutes at 300-500 g). After removing packaging cell debris...fibronectin-enhanced retroviral transduction of primary human T lymphocytes for adoptive immunotherapy." Acute Leukemias ...

  20. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...biology of the worm into a computer model that recapitulates how molecular biology impacts worm locomotion...generally funded on a membership model - everyone contributes each month - with only occasional supplementation...
Showing: 721 - 740 of 958 results