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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 935 results for: Kin

Showing: 721 - 740 of 935 results

  2. Hot Plasmids - October 2020

    Type
    Blog Post
    ... ability to study and understand the complex interworking of the 86 billion neurons in the human brain... opens the door to in vivo experiments such as tracking the synaptic maturation pattern of hippocampal...

  3. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...detection We and other members of the lab had been working on advancing the paper-based platform toward a ...when we needed additional expertise. Perhaps most striking is that there were seven institutions involved...

  4. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...has slightly different activation/inactivation kinetics. For example, the light-gated cation channel, ...protein-coupled vertebrate rhodopsin in cerebellar Purkinje cells. The Journal of biological chemistry. 2011...

  5. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...binding distinct PAM sequences. Now, Hu et al., working in David Liu’s lab, have gone one step further,...Once you’ve tried it, let us know how xCas9 is working for you in the comments section! References Hu...

  6. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...setup. This requires a less involved setup when working with mice and slightly less red tape for approval...has psuedotypes. Psuedotyping is the process of making a hybrid AAV, with the genome from one serotype...

  7. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ... fluorescent proteins.  Currently, the team is working to identify how MagLOV’s structure changes under...starting point for much more. If you’re interested in working on these questions or collaborating, the York Lab...

  9. Pooled Library Amplifications

    Type
    Blog Post
    ...bacteria can grow and compete in the same container, making it easier for a smaller, recombined plasmid to ...developing your amplification protocol! We recommend looking at protocols used for libraries that are similar...

  10. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ... side, successful indel generation and epitope knockin was achieved by a few respondents suggesting that...inconvenient. Another possible reason for the trickiness of NgAgo is the inability of mature NgAgo protein...

  12. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...the transformation efficiency of the strain: when asking the cell to perform multiple recombination events...recommend Phusion or Q5. Speed up your life Rather than making up your PCR mix every time, pre-made aliquots of...

  13. MXS Chaining

    Type
    Blog Post
    ...475nm Lyn-tag (derived from the tyrosine-protein kinase Lyn) Membranes 3 mCherry 587nm/ 610 nm human ...Anderson JC, Dueber JE, Leguia M, Wu GC, Goler JA, Arkin AP, et al. BglBricks: A flexible standard for biological...

  15. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...culture experience. Let’s go over the basics of making rAbs. Production Recombinant antibody production... because it secretes fewer of its own proteins, making the purification process simpler. Due to its high...

  16. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...don’t require a PAM sequence at the target locus, making them more flexible than Cas9/Cpf1. Some Cas13 enzymes...prefer targets with a given single base protospacer flanking site (PFS) sequence, but orthologs like LwaCas13a...

  18. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...Ampicillin, often just called amp, is the historical king of the antibiotic resistance realm. Amp is a semisynthetic...period after transformation (the dreaded 60 minutes shaking at 37 degrees) can be shortened since the plated...

  19. DNA Purification Without a Kit

    Type
    Blog Post
    ... bonds with water molecules and coaxes it into sticking to the hydrophobic glass filter. Each glass filter...depending on the size of bacterial culture you’re working with. Overall, the glass syringe filter method ...
Showing: 721 - 740 of 935 results