We narrowed to 766 results for: Ncl

...Protocol for creating a glycerol stock, including additional tips for long-term storage....the appropriate temperature. Growth conditions, including copy number and growth temperature, can be found...

...Protocol for how to purify DNA from an agarose gel, including tips to help improve the resolution and separation...increase the resolution (crispness) of your DNA bands include: a) running the gel at a lower voltage for a longer...

...to consider when selecting sequencing primers, including: Is the primer unique? Make sure the primer only...your plasmid, please email [email protected] and include: The sequencing result The original trace file ...

... You need to do this because you don’t want to include the weight of the weighing boat or paper into your...paper in the biohazard solid waste container. Conclusion Appropriately weighing reagents is important ...

...chew back" reaction, in which a single dNTP is included to stop the exonuclease function of the enzyme...manufacturer's instructions. In this case, we will include the free nucleotide dGTP in the reaction (exclude...

...A collection of Addgene's video content, including how-to screen captures, lab procedure protocols, and...some of the key strategies of aseptic technique, including personal protective equipment, setting up a clean...

... or recommend specific products or equipment. Inclusion of this information is solely for transparency... is both functioning as expected and specific, include a positive control sample that you know expresses...

...protocol for performing agarose gel electrophoresis, including tips to improve resolution and separation of bands...increase the resolution (crispness) of your DNA bands include: a) running the gel at a lower voltage for a longer...

...cloning by restriction enzyme digest (subcloning), including design and experimental procedures....Design (Choosing enzymes) Many DNA analysis tools, including Addgene’s Sequence Analyzer , allow you to identify...

... or recommend specific products or equipment. Inclusion of this information is solely for transparency...handling, use proper personal protective equipment including laboratory coat, laboratory goggles, and gloves...

... or recommend specific products or equipment. Inclusion of this information is solely for transparency... both functioning as expected and is specific, include a positive control sample that you know expresses...

... or recommend specific products or equipment. Inclusion of this information is solely for transparency... of the standard curve you will need to either include higher concentrations in the standard curve or ...

...without a kit. The general steps of this protocol include homogenization/lysis of cells or tissues, extraction...For the precipitation step, two options are also included: using Isopropanol or Lithium Chloride. This protocol...

...of Endura Duos, Lucigen, 60242-1) Alternatives include Stbl4 cells or other ultra-high efficiency electrocompetent..., gentler practices, and colder reagents (not including the recovery media!). Do not proceed with Maxipreps...

...efficiently infect a broad range of cell types, including both dividing and non-dividing cells. Addition...warranty of any kind either expressed or implied, including but not limited to the implied warranties of fitness...

...AATTAATT CCGCGCGG GTTAAC - 5' = 28 bp We also need to include additional bases to complement the overhangs generated...

...that cut your plasmid. Many DNA analysis tools, including Addgene’s Sequence Analyzer , allow you to identify...

...to save the pellet, the supernatant, or both. Conclusion Centrifuges are important tools for many different...

...Activity" and can happen for a variety of reasons, including high glycerol concentration. Learn more at (Link...

...transfection; therefore, plasmid DNA purification should include an endotoxin removal step. For high quality plasmid...