We narrowed to 961 results for: REP
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TypeBlog Post...ahead of time so that lab members have time to prepare their thoughts. Then at the lab meeting, the team...everyone stands up, disappears from the video, and prepares a coffee, or simply moves away from the desk to...
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5 Great Apps for Lab Life
TypeBlog Post...those of the guest blogger and do not necessarily represent the views of Addgene. I think it’s safe to assume...smartphone to help me with my research in terms of preparedness, organization, and sharing data. Here are five... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog Post...of simple biological machines R bodies can repeatedly extend and contract in response to changing pH... Biology Posts Suggest Plasmid Tools for the Repository Resources on Addgene.org Read our Hot Plasmids... -
Synthetic Photobiology: Optogenetics for E. coli
TypeBlog Post...phosphorylation, promoting transcription of a GFP reporter. Subsequent exposure to red light rapidly deactivates...larger systems. The work of Schmidl et al. also represents a large step forward in optimizing TCSs. Over... -
Advice for Scientists Starting a Lab
TypeBlog Post...enough that everyone wants to recruit rather than repel and hoard.” If you've already secured a faculty ...Tom Ellis has a one word answer: “Email. Nothing prepared me for what a PI without a personal assistant ... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog Post...of posts explains how to critically assess the reported properties of fluorescent proteins, how to do ...protein tagged with a fluorescent protein and repeating this with another fluorescent protein, a ranking... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog Post... information on each cancer clinical sequencing report includes rare variants that haven’t been characterized...MAPK1/ERK2 Missense Mutants. Brenan L et al. Cell Reports, 2016. PubMed PMID: 27760319. PubMed Central PMCID... -
Top Requested Lentivirus and AAV of 2016
TypeBlog Post...though we do provide a few non-CRISPR related viral preps. Cas9 is the headliner of most CRISPR applications...lentivirus. Secondly, you can use a LentiCas9-Blast viral prep to create a single stable Cas9-expressing cell line... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog Post...activated Ras/Erk signaling by using a fluorescent reporter for Erk kinase translocation. These results demonstrate...control protein activity Read about RANbodies, reporter nanobody fusions Learn more about using the secondary... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog Post... the most frequently used Cas9, spCas9 from Streptococcus pyogenes, this sequence is 3’-NGG. But Cas9s...Central PMCID: PMC6342934. Teng, Fei, et al. "Repurposing CRISPR-Cas12b for mammalian genome engineering... -
A Quick Guide to a Career in Software Product Management
TypeBlog Post...off for the day. 1:45 PM--Write final report. Work on a final report for a user research project I led. ... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog Post...populations or a huge smear across your FSH vs reporter plot. In this post, I’ll walk you through how ...Purification Protocol Video rAbs in the Addgene repository... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog Post...(nick, bind, cleave, mixed). This information represents the ‘big picture’ features of the enzymes documented...Classification System for Class 2 CRISPR-Cas Enzymes [Preprint]. Biochemistry, Biophysics, and Structural Biology... -
Viral Vectors 101: Types of viruses
TypeBlog Post...addition of a helper virus or plasmid allows it to replicate episomally, essentially remaining an extrachromosomal...extrachromosomal part of the host genome that can replicate and divide along with the endogenous DNA. AAV also... -
From Lab Bench to Leadership: Transitioning into Management as a PhD
TypeBlog Post...as exciting as it is challenging. How do you prepare for this shift and navigate the pitfalls? Here'...team success. This shift requires deliberate preparation and a willingness to embrace new responsibilities... -
How Addgene Creates Content
TypeBlog Post...entire mission is to enable that process. Our repository of plasmids, AAVs, antibodies, and data allows...and cited figures from papers or image sharing repositories. Then we format, do a final review to catch ... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog Post...which can then activate expression of a luciferase reporter in the nucleus. The Roth lab showed that this ... on neuron activity (firing or inhibition). AAV preps from DREADD plasmids allow you to easily express... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog Post...which there are never enough, especially in underrepresented groups) reach multiple mentees efficiently...pre-reads, videos or exercises to be completed in preparation for the meeting or to be read or watched during... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...research. But be aware that the fluorescent proteins reported in publications are only characterized under a...publishing it as (part of) figure 1, uploading it as a preprint, or documenting it in some other way. Besides ... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog Post... al., 2011). Additionally, researchers have reprogrammed regenerating tissues without using genome engineering...technologies are now available for fluorescent reporting and genetically encoded fluorescent voltage indicators...