We narrowed to 957 results for: REP

1. Protocol - How to Design Primers

   Type

   Protocol

   ...together. One must selectively block and unblock repeatedly the reactive groups on a nucleotide when adding...

2. A Quick Guide to a Career in Software Product Management

   Type

   Blog Post

   ...off for the day. 1:45 PM--Write final report. Work on a final report for a user research project I led. ...

3. GPCRs: How Do They Work and How Do We Study Them?

   Type

   Blog Post

   ...which can then activate expression of a luciferase reporter in the nucleus. The Roth lab showed that this ... on neuron activity (firing or inhibition). AAV preps from DREADD plasmids allow you to easily express...

4. From Lab Bench to Leadership: Transitioning into Management as a PhD

   Type

   Blog Post

   ...as exciting as it is challenging. How do you prepare for this shift and navigate the pitfalls? Here'...team success. This shift requires deliberate preparation and a willingness to embrace new responsibilities...

5. Flow Cytometry Readouts: Yes, No, and Everything in Between

   Type

   Blog Post

   ...populations or a huge smear across your FSH vs reporter plot. In this post, I’ll walk you through how ...Purification Protocol Video rAbs in the Addgene repository...

6. A Practical Approach to Choosing the B(right)est Fluorescent Protein

   Type

   Blog Post

   ...of posts explains how to critically assess the reported properties of fluorescent proteins, how to do ...protein tagged with a fluorescent protein and repeating this with another fluorescent protein, a ranking...

7. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

   Type

   Blog Post

   ... information on each cancer clinical sequencing report includes rare variants that haven’t been characterized...MAPK1/ERK2 Missense Mutants. Brenan L et al. Cell Reports, 2016. PubMed PMID: 27760319. PubMed Central PMCID...

8. Top Requested Lentivirus and AAV of 2016

   Type

   Blog Post

   ...though we do provide a few non-CRISPR related viral preps. Cas9 is the headliner of most CRISPR applications...lentivirus. Secondly, you can use a LentiCas9-Blast viral prep to create a single stable Cas9-expressing cell line...

9. CasPEDIA: A Functional Classification of Cas Enzymes

   Type

   Blog Post

   ...(nick, bind, cleave, mixed). This information represents the ‘big picture’ features of the enzymes documented...Classification System for Class 2 CRISPR-Cas Enzymes [Preprint]. Biochemistry, Biophysics, and Structural Biology...

10. Viral Vectors 101: Types of viruses

    Type

    Blog Post

    ...addition of a helper virus or plasmid allows it to replicate episomally, essentially remaining an extrachromosomal...extrachromosomal part of the host genome that can replicate and divide along with the endogenous DNA. AAV also...

11. How Addgene Creates Content

    Type

    Blog Post

    ...entire mission is to enable that process. Our repository of plasmids, AAVs, antibodies, and data allows...and cited figures from papers or image sharing repositories. Then we format, do a final review to catch ...

12. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type

    Blog Post

    ...activated Ras/Erk signaling by using a fluorescent reporter for Erk kinase translocation. These results demonstrate...control protein activity Read about RANbodies, reporter nanobody fusions Learn more about using the secondary...

13. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type

    Blog Post

    ... the most frequently used Cas9, spCas9 from Streptococcus pyogenes, this sequence is 3’-NGG. But Cas9s...Central PMCID: PMC6342934. Teng, Fei, et al. "Repurposing CRISPR-Cas12b for mammalian genome engineering...

14. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type

    Blog Post

    ...which there are never enough, especially in underrepresented groups) reach multiple mentees efficiently...pre-reads, videos or exercises to be completed in preparation for the meeting or to be read or watched during...

15. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type

    Blog Post

    ...research and how they will use plasmids and viral preps from Addgene to further their work. The Association...catalog would allow us to enhance our biosensor repository as we find ourselves lacking several crucial ...

16. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type

    Blog Post

    ... about being a science writer?” to which Susan replied, “What’s a science writer?” Learning about SciComm...donors such as fundraising materials and progress reports, in addition to articles for the Fred Hutch website...

17. Seven Tips for Using LinkedIn as a Scientist

    Type

    Blog Post

    ...requests without a personal message and they may report you as spam or “I Don’t Know This Person.” If that...profile and not updating it. LinkedIn may actually replace the resume some day (I’ve already abandoned my ...

18. Troubleshooting Your Plasmid Cloning Experiment

    Type

    Blog Post

    ...strain is growing fine, it is probably time to prepare new plates with a fresh stock of antibiotic. Many...can use the chromatogram to spot whether any discrepancies between your sequencing result and the expected...

19. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type

    Blog Post

    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...rapidly screen thousands of proteins for one that can report electrical activity via imaging. They took the ...

20. Transferable Skills Guide: Problem Solving

    Type

    Blog Post

    ...problem, you might even be expected to write up a report to communicate your findings to your colleagues... of contexts. As such, scientists are uniquely prepared to be strong problem solvers both in and out of...