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We narrowed to 961 results for: REP

Showing: 721 - 740 of 961 results
  1. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...those of the guest blogger and do not necessarily represent the views of Addgene. I think it’s safe to assume...smartphone to help me with my research in terms of preparedness, organization, and sharing data. Here are five...
  2. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...of simple biological machines R bodies can repeatedly extend and contract in response to changing pH... Biology Posts  Suggest Plasmid Tools for the Repository Resources on Addgene.org Read our Hot Plasmids...
  3. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...phosphorylation, promoting transcription of a GFP reporter. Subsequent exposure to red light rapidly deactivates...larger systems. The work of Schmidl et al. also represents a large step forward in optimizing TCSs. Over...
  4. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...enough that everyone wants to recruit rather than repel and hoard.” If you've already secured a faculty ...Tom Ellis has a one word answer: “Email. Nothing prepared me for what a PI without a personal assistant ...
  5. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...though we do provide a few non-CRISPR related viral preps. Cas9 is the headliner of most CRISPR applications...lentivirus. Secondly, you can use a LentiCas9-Blast viral prep to create a single stable Cas9-expressing cell line...
  6. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...(nick, bind, cleave, mixed). This information represents the ‘big picture’ features of the enzymes documented...Classification System for Class 2 CRISPR-Cas Enzymes [Preprint]. Biochemistry, Biophysics, and Structural Biology...
  7. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...addition of a helper virus or plasmid allows it to replicate episomally, essentially remaining an extrachromosomal...extrachromosomal part of the host genome that can replicate and divide along with the endogenous DNA. AAV also...
  8. How Addgene Creates Content

    Type
    Blog Post
    ...entire mission is to enable that process. Our repository of plasmids, AAVs, antibodies, and data allows...and cited figures from papers or image sharing repositories. Then we format, do a final review to catch ...
  9. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...which can then activate expression of a luciferase reporter in the nucleus. The Roth lab showed that this ... on neuron activity (firing or inhibition). AAV preps from DREADD plasmids allow you to easily express...
Showing: 721 - 740 of 961 results