We narrowed to 883 results for: prot

...bacteria, yeast can post-translationally modify proteins yet they still share many of the same technical...

...antibody, CRISPR, disease research, visualization, protein expression, synthetic biology, and viral vector...Collection Visualization Michael Davidson Fluorescent Protein Collection Allen Institute for Brain Science AAV... Collection Botman-Teusink Yeast FP Collection Protein Expression Cell Migration Consortium Malate Dehydrogenase...Reagents for improved expression of recombinant phosphoproteins - Rinehart FreeGenes Project Open Enzyme Collection...

...observations about the issue, assessed potential protocol modifications, and ultimately decided on the changes...

.... Serotype refers to the type of capsid (outer protein shell) used to package an rAAV vector; these capsids...

...single-panel figures, one of which was a cartoon of the protein.) In the middle of my presentation, lost in a sea...

... the work you do to make sharing reagents and protocols so easy!” — Nina Jain, Harvard Medical School....

... errors occur), deletions, mutations, in-frame protein fusions, and multiple cloning sites. In the simplest...

...from Addgene here! Future possibilities for Cas proteins With their enhanced specificity, eSpCas9, SpCas9...

...in a single reaction. For detailed Golden Gate protocols, complete with helpful tips and tricks, see The...

...explains 1-2 potential pitfalls of the intended protocol, and offers alternative solutions to demonstrate...

...AAV, work has focused on modifying the outside protein shell, or capsid, of the virus so that it only ...

...from the benefit of resistance plasmids, which protect the host from attacks by other organisms. To conserve...

... However, since the lab was highly focused on protecting intellectual property, it worked away in isolation...

...exonuclease protection factor La to PEmax to enhance pegRNA stability epegRNA — additional protection added ...PE2 plus additional sgRNA PE4 — PE2 plus MLH1 (a protein component of MMR) mutant to inhibit MMR PE5 — PE3...reference page . CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content...

...can be safely handled at the lowest biosafety protection levels. We offer 1,077 in-stock viral vector ... your AAV experiments, remember to share your protocols and results through Addgene’s Data Hub . By contributing...globe and shared our well-developed and trusted protocols and peer-reviewed techniques (Link opens in a ...preps. Data Hub Access Addgene’s Data Hub for protocols and results shared by other scientists using AAV...

...technology for targeting enzymes and other useful protein domains to a specific DNA sequence ( Figure 1A ...Biotechnology in August, uses a serial ligation protocol to assemble arrays. Dr. Joung, co-founder of the...TAL effector arrays in a 2012 issue of Nature Protocols . More information and help can be found at TALeffector...Pubmed PMID: 21822241 . TAL effectors: customizable proteins for DNA targeting Bogdanove AJ, Voytas DF. Science...

... and capsid proteins necessary for AAV vector production and assembly. The capsid protein encoded in the...production and assembly of viral capsids. The capsid protein encoded in the packaging plasmid will determine... Videos Addgene's Biosafety Resources Addgene Protocols General Transfection AAV Production in HEK293T...

...of DNA to make RNA, translation of RNA to make proteins. That's central to everything that goes on inside...

...the same page in terms of the vocabulary--DNA, protein, plasmid, gene, etc. Though I talked about CRISPR...

...processes: cell wall synthesis, DNA replication, or protein synthesis. Because they target such generic processes...