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We narrowed to 749 results for: rev

Showing: 721 - 740 of 749 results
  1. Centrifugation

    Type
    Protocol
    ...given as Relative Centrifugal Force (RCF) or Revolutions Per Minute (RPM), these are not the same units...
  2. Immunocytochemistry

    Type
    Protocol
    ...antibody concentration will vary between antibodies. Review the manufacturer's instructions before starting...
  3. Western Blot

    Type
    Protocol
    ...and blocking buffer will vary between antibodies. Review the instructions before starting your experiment...
  4. Technical Design of a Western Blot

    Type
    Blog Post
    ...performing your own validation assays. If you’re using previously published antibodies, check the manuscript and...
  5. CRISPR Guide

    Type
    Guide
    ...the donor template, incorporating mutations that prevent further Cas9 cleavage will improve the accuracy... method in which Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is fused to the C-terminus...the desired edit is incorporated into the DNA by reverse transcription. The edited DNA strand displaces ...editing. PASTE uses a CRISPR-Cas9n fused to both a reverse-transcriptase and a serine integrase (Bxb1), along...dCas9-mediated gene activation or repression is reversible, as it does not permanently modify the genomic...multiple fusions. The effects of CRISPRi/a may be reversible once the effector is inactivated or removed from... and even cell differentiation. The system is reversible, with a companion CRISPRon to undo repression...
  6. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...round of reverse transcription. After incorporation into a host cell, this deletion prevents further transcription...retroviruses is made of RNA. This retroviral RNA is reverse transcribed into DNA before being integrated in...genome of a host. Going from RNA to DNA is the reverse of what you would expect in biology — hence the...–11 kb, encoded on RNA (Figure 1). This RNA is reverse transcribed into the provirus, the cDNA that will...components. pol in trans Precursor protein containing reverse transcriptase and integrase components. Envelope...Engineered for safety and efficiency. Content last reviewed: 9 June 2025...
Showing: 721 - 740 of 749 results