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We narrowed to 971 results for: lars;

Showing: 371 - 380 of 971 results

  1. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ... very specific biological targets. However, as popular and useful antibodies are, they are in a midst ...as non-human primates and humans. Since we need larger-volume antibodies for thicker tissues and whole... immunoglobin component that can be produced in large “copies.” This homogeneity circumvents the batch-to-batch...reproducibility and cost-effectiveness for broad and large quantity uses. We therefore use both rAb and mAB...same target to see if the results are the same/similar, emphasizing the antibody’s specificity. Strength...from the background noise. If the sample images similarly to the negative control, that informs us that ...

  2. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...polyadenylation signal by using in vitro analyses. Molecular and Cellular Biology. 1992. PMID: 1333042. PubMed Central... thought that poly(A)s are used to control the cellular concentration of regulatory RNAs and may additionally...secondary structure to induce transcript cleavage, similar to the Rho-independent termination found in prokaryotes...terminate transcription. Polymerase I uses a process similar to the prokaryotic Rho-dependent mechanism, whereas...Terminators: The RNA 3′-End Chronicles. Journal of molecular biology. 2011. PMID: 21439297. PubMed Central ...

  3. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...Simon! Derek P. Simon received his PhD in Cellular and Molecular Biology from the University of Michigan...blogger Derek Simon. The surgeries and standard molecular neuroscience validation experiments we discussed...cemented to the animal’s skull using dental cement (a similar procedure as implanting a guide cannula). A fiber... Gi/o protein-coupled vertebrate rhodopsin in cerebellar Purkinje cells. The Journal of biological chemistry...PMCID: PMC3466483. 4. Kim TI, et al. Injectable, cellular-scale optoelectronics with applications for wireless...

  4. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...cells are a great option for viral production, particularly for labs that already do mammalian cell culture...for the generation of viruses, though they are a popular choice for protein production. The most commonly...infect insects. This is what makes insect cells a popular choice for protein production. AAV and lentiviruses...systems. Baculovirus transfer plasmids have a much larger packaging capacity — up to 38 kb! Generally, insect...in between passages to eliminate dead cells and cellular debris. While adding time (about three to four...supernatant in a centrifuge to remove any unwanted cellular debris, as you would when collecting a lentivirus...

  5. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...specific genes in mammalian cells. Despite the popularity of CRISPR-based knockout/knock-in systems, these...nucleotide sequences are exchanged between two similar or identical molecules of DNA. Gene targeting takes...targeting constructs. In the example shown in Figure 1, large sequences corresponding to exons 1 and 3 of the ... the cells that have randomly integrated all or large portions of the plasmid. Deliver your construct ...insert GFP into the genome, we’d create a plasmid similar to the one shown below, where the sequence of GFP...CRISPR is very good at making knockouts, knocking in large sections of DNA can be more difficult. Addgene depositors...

  6. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ... that can bind effectively to protein targets, similar to how antibodies do. The structure of the common...(~150 kDa) and Y-shaped because it contains two large heavy chains and two small light chains that stick...antibodies to get to, like ones that are hidden in the molecular crevices proteins on the cell surface. Figure...optimally binding sybody, you want to start out with a large and diverse pool of sybodies that hypothetically...because it has the advantage of starting out with a larger initial starting library size (up to 1012 members...Characterization of Enzyme Variants. In: Methods in Molecular Biology. Humana Press, pp 133–148. https://doi.org...

  7. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...already a packaging capacity larger than AAV vectors. This capacity is large enough to carry the Cas9 gene...compared to other viral vectors, AdVs can provide a large transgene packaging capacity. For instance, with...non-transformed cells yielded rates of targeted mutagenesis similar to those achieved by isogenic AdVs encoding TALENs... B-thalassemia, Sickle Cells Disease, Duchenne Muscular Dystrophy, Alpha1-antitrypsin deficiency (most... animals in order to create animal models for particular cancer. That’s exactly what the lab of Andrea...

  8. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    ...into embryos and then become larvae. There are three larval stages where larvae molt in order to continue...continue growing in the next larval stage. After the third larval stage, Drosophila will pupate, which takes...fertilized egg by its mother. Inside the pupal case, the larva will metamorphose into its adult form. In all, it...

  9. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ... might need to use multiple techniques for a particularly stubborn prep. Check the bacterial strain Increase...strains, including HB101 and derivatives, release large amounts of carbohydrates that can inhibit the cell...levels of protein, not plasmid DNA. If you need large amounts of DNA from a plasmid in a protein expression...choice of strain will ultimately depend on the particular features of your plasmid, it is advisable to ...coli strains. Increase the volume Starting with a larger culture volume can improve plasmid yield. Our lab...gas exchange in the overnight culture. Generally, large cultures in flasks may be shaken around 220 RPM,...

  10. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...depositing lab. There may be other plasmids with similar names in the collection, but you can use this name...some of Addgene’s plasmids is an indication of popularity. A yellow flame indicates that a plasmid has ... on our website, one of the available filters popularity (e.g. only showing those plasmids which have ... than 100 times).     I guess it's cool to be popular, but how do I know if a plasmid is actually useful...If the depositor has indicated that there is a particular mutation found within the gene/insert, this information...means you can see which labs have already used a particular plasmid, and which experimental systems it was...
Showing: 371 - 380 of 971 results