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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 958 results for: Tes

Showing: 741 - 760 of 958 results

  3. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ..., VWR 76322-154 Pipettes, 5 mL, VWR 89130-896 Pipettes, 10 mL, VWR 89130-898 Pipettes, 25 mL, VWR 89130...of our protocols supports reproducibility and accelerates science. Here, we list the specific equipment...89130-900 Pipettes, 50 mL, VWR 89130-902 Gel loading tips, Corning 4853 Microcentrifuge tubes, Neptune 3745...mobility of the bands (sample AR0016 in Figure 1) indicates that the samples may not have been processed correctly...

  4. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...also enabled imaging of their propagation along dendrites for the first time (Figure 3). The team also used...a patient with non-neurodegenerative diseases (Diabetes & cancer). Image adapted from Wu and Wang 2024...

  5. Plasmids 101: Degron Tags

    Type
    Blog Post
    ... compared to other systems because the system modulates protein post-translationally, unlike many other...through interacting ligands. After an E3 ligase facilitates ubiquitination of the Halo-tagged protein in ...

  6. PhD Applications After COVID

    Type
    Blog Post
    ...applied to PhD programs in Biology around the United States in 2021, I know that this process only became even...program you’ll be using for the interview. Having notes close to your computer with things you want to highlight...

  7. Transferable Skills: Negotiation

    Type
    Blog Post
    ...(Can I attend this conference?) and with my lab mates on which lab jobs needed to be done (Who is responsible...your needs actually are: Maybe you only need 15 minutes of the two hour time slot, so she can take the ...

  9. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...inside and outside your field. Your colleagues, lab mates, and PI can provide feedback on your technical presentation...pressed for time, limit yourself to five to ten minutes per poster. Ask the presenter to give the “elevator...

  12. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...sequence. Co-delivery of wild-type Cas9 and a gRNA generates a double-strand break in the target DNA, which...experiment, the use of appropriate controls, multiple replicates and several cell types can strengthen your results...

  13. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...using a pinhole.  However, the excitation light excites all of the fluorophores as it passes through the...the laser light sheet, typically 2-6  microns, illuminates only one thin plane of the sample surrounding...

  15. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...Aligning two nucleotide sequences is probably the fastest BLAST search to perform and will save you time ...comparing different isoforms. In addition to our favorites, there are a number of sequence alignment tools...

  16. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...Figure 3: The life cycle of a retrovirus. Image courtesy of Jeremy Luban, MD. Figure 3 depicts the ... the National Academy of Sciences of the United States of America, 54(1), 137–144. https://doi.org/10.1073...

  18. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...results in preclinical studies some potential candidates for rare to ultra-rare ocular diseases are being... of new capsid variants, designing expression cassettes to regulate and optimize transgene levels, and...

  19. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...study by the NSF showed that among US 4th graders tested on the Science National Assessment of Educational... you don’t have children but are interested in testing your SciComm skills or are interested in a career...

  20. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...within the folded protein (green region). Methanol dehydrates the protein, which can disrupt protein structure...at your antibody datasheet to see if it has been tested in IF and if a protocol is available. When possible...
Showing: 741 - 760 of 958 results