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We narrowed to 935 results for: Kin

Showing: 741 - 760 of 935 results

  2. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...library screen only to find the system itself is not working because your reporter protein doesn’t function ...Rajagopala S.V., Stellberger T., Uetz P.  Exhaustive Benchmarking of the yeast two-hybrid system. Nature Methods...

  3. DNA Purification Without a Kit

    Type
    Blog Post
    ... bonds with water molecules and coaxes it into sticking to the hydrophobic glass filter. Each glass filter...depending on the size of bacterial culture you’re working with. Overall, the glass syringe filter method ...

  4. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...and develop an area of expertise. Whether you’re seeking a full-time job or want to be an independent grant...a mentor, find your people Find someone who is working or has worked as a Grant Professional to mentor...

  5. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...optogeneic actuators and sensors at Addgene. Cell marking/selection: Expression constructs like plasmids ...Because of its stability, GFP can be used in lineage tracking capacities in cell fate studies. It can also be...

  6. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...many questions from scientists all over the world looking for some additional information or clarification...functional before investing time and resources into making a stable cell line. When expressing a viral construct...

  7. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...The assay only uses one antigen binding antibody, making it less specific than other methods. In addition...positive control will confirm that the procedure is working and should always be included.  For a negative...

  8. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...lentivirus to be time consuming, difficult, and lacking in reproducibility. Read on for some tips for getting...overly confluent or too old. Ensuring that you are working with healthy, happy cells is one of the easiest...

  9. Hot Plasmids - August 2020

    Type
    Blog Post
    ...the yeast extracellular surface) which enables tracking of protease activity with a fluorescent protein...that the best motif was from the Lck gene. For marking microtubules of mammalian cells, the author found...

  12. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...research vision uninterrupted and then you will begin working through your aims over the next 45 minutes. The...salient details. Make the most of your limited speaking time. Beyond the information you seek to convey...

  13. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...high fidelity during action potentials of fast-spiking neurons. The same A-to-D mutation in the related...useful materials for research into Alzheimer’s, Parkinson’s, ALS, and Huntington’s Disease, including plasmids...

  14. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ... Specific High Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK and SHERLOCKv2) (Gootenberg et al., ... Specific High Sensitivity Enzymatic Reporter UnLOCKING Cas13 (C2c2) was first identified in 2016 by the...

  15. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...tools for cellular targeting. Lately, they’ve been working on two tools that expand and exploit CRISPR, both...Jonathan and experts in CRISPR evolution, were also working on the project. Using computational observations...

  16. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ... used as a test to see how well treatments are working to fight the cancer.  Beyond early modifications...Asbestos Disease Awareness Organization (ADAO) are working toward such a ban, and the president of the organization...

  17. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...positive selection and a p15A origin of replication, making it incompatible with other p15A plasmids. pLys ...of Replication The Promoter Region – Let's Go! Looking for More Plasmids 101 Articles?  Find them all ...

  18. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...biologist you would say. The student was of course talking about the now well-known FRET, aka Fluorescence...and the extinction coefficient of the acceptor (linking the quantity of absorbed light, at a given wavelength...

  19. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...thoughts on research statements. Snapp, who is always looking for ways to improve his eBook, adds: "If you have... fit into a department and bring in grants. What kind of colleague will you be? Do you understand what...
Showing: 741 - 760 of 935 results