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...other. Phusion High-Fidelity DNA Polymerase - incorporates nucleotides to “fill in” the gaps in the annealed... product by restriction digest . Sequence the important regions of your final plasmid, particularly the...

...that sharing the full details of our protocols supports reproducibility and accelerates science. Here,...information is solely for transparency intended to support reproducibility in science. Last Update: January...

...Addgenie Leila Haery has summed up some of the most important characteristics of retroviruses, lentiviruses,...

...access to IPI educational resources developed to support use of their antibody tools. Addgene also offers...

...series! Follow @addgene on TikTok for bite-sized portions of antibody concepts. Each short video answers...

...connect with other researchers working with AAV, support open science, and maybe even win a $500 travel ...

...and vote for your favorite! Our mascot who has supported us over the past eight years now has a new friend...

...Conclusion Pipetting accurately and precisely is important since even small changes in the volume could affect...Beyond knowing the proper way to pipette, it’s important for scientists to calibrate their pipettes to ...

...Experiences (CUREs)Author: Jason PellettieriNew Acoustic Reporter Genes: Ultrasound Imaging of Gene ExpressionAuthors...

...organelles, lipoproteins, and macromolecules. Importantly, iodixanol is inert and non-toxic to mammalian...and unlike other density gradient media, it is reportedly not necessary to remove iodixanol prior to use...

... students and teachers interested in JEI (the importance of word of mouth and personal networking!) and...

...working with Andrew, the head of our scientific support team, to tackle any experimental questions you ...

...trick When combining reagents in the lab, it is important to mix them thoroughly before use.  For efficient...

...experiences. It will contain all of the juicy and important details that you won’t find in the final publication...

...introduced us to RUBY-red, a bright red pigment reporter for plants.  Cas7-11 and PASTE: Omar Abudayyeh...

...Generating DNA Molecular Weight Markers. Scientific Reports,7(1). doi:10.1038/s41598-017-02693-1. PubMed PMID...

... of 10 mL, we’ll want 200 mL of media total. Importantly, we’ll actually make a bit more than 200 mL (...kill most potential contaminants, certain types of spores will stubbornly survive even under the harsh conditions...

...that sharing the full details of our protocols supports reproducibility and accelerates science. Here,...information is solely for transparency intended to support reproducibility in science. General Guidelines ...

...background and education 3:49-4:35 : Kingdom on opportunities to go into science in Malawi 4:36-5:46 : How...

...18265393. Additional Resources on the Addgene Blog Important Considerations When Using AAVs AAVs Are Great ...