We narrowed to 957 results for: REP
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TypeBlog Post...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...rapidly screen thousands of proteins for one that can report electrical activity via imaging. They took the ...
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Seven Tips for Using LinkedIn as a Scientist
TypeBlog Post...requests without a personal message and they may report you as spam or “I Don’t Know This Person.” If that...profile and not updating it. LinkedIn may actually replace the resume some day (I’ve already abandoned my ... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog Post...strain is growing fine, it is probably time to prepare new plates with a fresh stock of antibiotic. Many...can use the chromatogram to spot whether any discrepancies between your sequencing result and the expected... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...research. But be aware that the fluorescent proteins reported in publications are only characterized under a...publishing it as (part of) figure 1, uploading it as a preprint, or documenting it in some other way. Besides ... -
Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog Post...research and how they will use plasmids and viral preps from Addgene to further their work. The Association...catalog would allow us to enhance our biosensor repository as we find ourselves lacking several crucial ... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog Post...of Research on Women’s Health, emphasizes that reporting the sex of cells and tissues—when that information...diagnostics and therapeutic interventions you develop represent the whole human population. To learn a little ... -
Career Coaching for Scientists: Why and Where Do I Find One?
TypeBlog Post...Thesis (who has also blogged for us) offers a free report on her website called “Secrets to Success in Graduate...development to mentoring for women and under-represented minorities. Her online content is of great value... -
Antibodies 101: Choosing the Right Isotype
TypeBlog Post...lot to lot specificity and tend towards more reproducible results. Since polyclonal antibodies contain... reactive ones. And make sure to check for any reported cross-reactivity when planning your experiment... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog Post...To expand the targeting scope of ABE8, the team replaced S. pyogenes Cas9 in the ABE complex with PAM-variant...selection of guideRNAs. Of particular note, ABE8s reported in this work did not cause genome-wide, guide-... -
Mouse Modeling, Part 2: Breeding and Crossing Mice
TypeBlog Post...problems with transgenic mice that can impact reproduction include embryonic lethality, a short lifespan... to mouse modeling now complete, you should be prepared to plan an experimental strategy that uses genetically... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post... (abundant in eukaryotes) A Near-Infrared BiFC Reporter for In Vivo Imaging of Protein-Protein Interactions...green-yellow or orange-red fluorogen A split fluorescent reporter with rapid and reversible complementation. Tebo... -
Common Injection Routes in Mice
TypeBlog Post...finally, to expert. Figure 1. Visual representation of common injection routes in mice. Image created...events. Intradermal injections require a bit of extra prep, as the injection site area must be shaved first... -
PhD Applications After COVID
TypeBlog Post...transcripts if requested Thank them, and eagerly await a reply. It’s a good idea to email around three professors...about your research goals and education, too! Prepping for the interview Many universities offer free... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog Post... design, there are few tools to analyze CRISPR repair outcomes, which is important for quality control...analysis because of its ease-of-use and comprehensive reports. With CrispyCrunch, you get the all information... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog Post...have been around since 2000 when Terskikh et al. reported the production of dsRed E5. This timer predictably...fluorescent timers that change color from blue to red report on cellular trafficking." Nature chemical biology... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog Post...work, and it begins with the central dogma - replication of DNA, transcription of DNA to make RNA, translation... tame the rest of a cell. The BIOFAB plasmids represent the first systematic collection of genetic parts... -
The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data
TypeBlog Post...dramatically when there are many new COVID-19 cases being reported (right, red), likely due to quarantining {%...noiseless) model for our data: We choose to let yx(t) represent the number of unique PIs or labs placing orders... -
A Guide to Starting Your Own Journal Club
TypeBlog Post...club? Where will the journal club take place? Preparing for the first meeting There are many important...include the paper, any supplemental data, software repositories, or other information needed by someone to critically... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog Post...cell types, gene editing using homology directed repair (HDR), and generating large genomic deletions. ...delivery. You’ve decided to use RNPs, now what? Preparing the Cas9-gRNA ribonucleoprotein The first step... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog Post... programmability of CRISPR-Cas, these systems represent the next generation of tools for efficient and...genomes engineering In the Sternberg group’s initial report of VchINT, three separate plasmids and multiple...