We narrowed to 961 results for: REP
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TypeBlog Post...requests without a personal message and they may report you as spam or “I Don’t Know This Person.” If that...profile and not updating it. LinkedIn may actually replace the resume some day (I’ve already abandoned my ...
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Troubleshooting Your Plasmid Cloning Experiment
TypeBlog Post...strain is growing fine, it is probably time to prepare new plates with a fresh stock of antibiotic. Many...can use the chromatogram to spot whether any discrepancies between your sequencing result and the expected... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...rapidly screen thousands of proteins for one that can report electrical activity via imaging. They took the ... -
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute
TypeBlog Post... about being a science writer?” to which Susan replied, “What’s a science writer?” Learning about SciComm...donors such as fundraising materials and progress reports, in addition to articles for the Fred Hutch website... -
Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog Post...research and how they will use plasmids and viral preps from Addgene to further their work. The Association...catalog would allow us to enhance our biosensor repository as we find ourselves lacking several crucial ... -
Transferable Skills Guide: Problem Solving
TypeBlog Post...problem, you might even be expected to write up a report to communicate your findings to your colleagues... of contexts. As such, scientists are uniquely prepared to be strong problem solvers both in and out of... -
What's New in CRISPR - November 2021
TypeBlog Post...their Cell paper, the Liu Lab found that mismatch repair (MMR) interfered with the desired resolution of...Biotechnology Find the PRIME-del plasmids Deletion and replacement of long genomic sequences The PEDAR strategy ... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog Post...strand DNA generation, such as homology directed-repair. Cas12a is also conducive for targeting AT-rich...requirement: TTN or TTTN. Best for: homology-directed repair or therapeutic delivery. Cas14 – Mini, but single... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog Post...that this small change made a big difference. Replacing the mCherry with the HA tag resulted in abundant...the experiment. Since the fused protein could represent a hindrance to the successful expression of the... -
Gendered Innovations: Why Does Sex of the Cell Matter?
TypeBlog Post...of Research on Women’s Health, emphasizes that reporting the sex of cells and tissues—when that information...diagnostics and therapeutic interventions you develop represent the whole human population. To learn a little ... -
Career Coaching for Scientists: Why and Where Do I Find One?
TypeBlog Post...Thesis (who has also blogged for us) offers a free report on her website called “Secrets to Success in Graduate...development to mentoring for women and under-represented minorities. Her online content is of great value... -
Mouse Modeling, Part 2: Breeding and Crossing Mice
TypeBlog Post...problems with transgenic mice that can impact reproduction include embryonic lethality, a short lifespan... to mouse modeling now complete, you should be prepared to plan an experimental strategy that uses genetically... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog Post...To expand the targeting scope of ABE8, the team replaced S. pyogenes Cas9 in the ABE complex with PAM-variant...selection of guideRNAs. Of particular note, ABE8s reported in this work did not cause genome-wide, guide-... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post... (abundant in eukaryotes) A Near-Infrared BiFC Reporter for In Vivo Imaging of Protein-Protein Interactions...green-yellow or orange-red fluorogen A split fluorescent reporter with rapid and reversible complementation. Tebo... -
Antibodies 101: Choosing the Right Isotype
TypeBlog Post...lot to lot specificity and tend towards more reproducible results. Since polyclonal antibodies contain... reactive ones. And make sure to check for any reported cross-reactivity when planning your experiment... -
Plasmid Modification by Annealed Oligo Cloning (with Protocols)
TypeProtocol...plate. Be sure to pick multiple colonies for mini-prepping and verify insert by sequencing. Reference Page... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog Post...have been around since 2000 when Terskikh et al. reported the production of dsRed E5. This timer predictably...fluorescent timers that change color from blue to red report on cellular trafficking." Nature chemical biology... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog Post... design, there are few tools to analyze CRISPR repair outcomes, which is important for quality control...analysis because of its ease-of-use and comprehensive reports. With CrispyCrunch, you get the all information... -
Common Injection Routes in Mice
TypeBlog Post...finally, to expert. Figure 1. Visual representation of common injection routes in mice. Image created...events. Intradermal injections require a bit of extra prep, as the injection site area must be shaved first... -
PhD Applications After COVID
TypeBlog Post...transcripts if requested Thank them, and eagerly await a reply. It’s a good idea to email around three professors...about your research goals and education, too! Prepping for the interview Many universities offer free...