Skip to main content
Addgene

We narrowed to 961 results for: REP

Showing: 741 - 760 of 961 results
  1. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...requests without a personal message and they may report you as spam or “I Don’t Know This Person.” If that...profile and not updating it. LinkedIn may actually replace the resume some day (I’ve already abandoned my ...
  2. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...strain is growing fine, it is probably time to prepare new plates with a fresh stock of antibiotic. Many...can use the chromatogram to spot whether any discrepancies between your sequencing result and the expected...
  3. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...problem, you might even be expected to write up a report to communicate your findings to your colleagues... of contexts. As such, scientists are uniquely prepared to be strong problem solvers both in and out of...
  4. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...their Cell paper, the Liu Lab found that mismatch repair (MMR) interfered with the desired resolution of...Biotechnology Find the PRIME-del plasmids  Deletion and replacement of long genomic sequences The PEDAR strategy ...
  5. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...strand DNA generation, such as homology directed-repair. Cas12a is also conducive for targeting AT-rich...requirement: TTN or TTTN. Best for: homology-directed repair or therapeutic delivery.   Cas14 – Mini, but single...
  6. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...of Research on Women’s Health, emphasizes that reporting the sex of cells and tissues—when that information...diagnostics and therapeutic interventions you develop represent the whole human population. To learn a little ...
  7. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...Thesis (who has also blogged for us) offers a free report on her website called “Secrets to Success in Graduate...development to mentoring for women and under-represented minorities. Her online content is of great value...
  8. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...problems with transgenic mice that can impact reproduction include embryonic lethality, a short lifespan... to mouse modeling now complete, you should be prepared to plan an experimental strategy that uses genetically...
  9. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...lot to lot specificity and tend towards more reproducible results. Since polyclonal antibodies contain... reactive ones. And make sure to check for any reported cross-reactivity when planning your experiment...
  10. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...have been around since 2000 when Terskikh et al. reported the production of dsRed E5. This timer predictably...fluorescent timers that change color from blue to red report on cellular trafficking." Nature chemical biology...
  11. Common Injection Routes in Mice

    Type
    Blog Post
    ...finally, to expert.   Figure 1. Visual representation of common injection routes in mice. Image created...events. Intradermal injections require a bit of extra prep, as the injection site area must be shaved first...
  12. PhD Applications After COVID

    Type
    Blog Post
    ...transcripts if requested Thank them, and eagerly await a reply. It’s a good idea to email around three professors...about your research goals and education, too! Prepping for the interview Many universities offer free...
Showing: 741 - 760 of 961 results