Plasmids 101: Optimizing Plasmid Yields
Type
Blog Post
...of endA+ strains
Strains like Stbl3 have been engineered to reduce plasmid recombination, but are also...
We narrowed to 809 results for: eng
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Experimenting with New Careers while in Grad School
TypeBlog Post...realized I wanted a career in science outreach: engaging the public to promote an awareness and understanding... -
Genome-wide Screening Using CRISPR
TypeBlog Post...multiple replicates and several cell types can strengthen your results. Enrichment or depletion of multiple... -
Light Sheet Fluorescence Microscopy
TypeBlog Post...C.D. Hojer, J.G. Egen, F. Hellal, F. Bradke, M. Sheng, H.-U. Dodt. (2012). Nature Protocols, 7(11), 1983... -
Tips for Using BLAST to Verify Plasmids
TypeBlog Post...included in the search to maximize the alignment length. While this recommendation is not infallible, we... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...also theoretically possible, but likely more challenging, with lentiviral expression. For more detail ... -
Getting to Know Expansion Microscopy
TypeBlog Post...traditional full IgG secondaries, can improve signal strength (Sherry and Stiles, 2022). Fourth, signal continuity... -
Simplify Cloning with in vivo Assembly
TypeBlog Post...temperature (Tm) of around 60 °C for all primers. The length of the homologous region should be around 20 bp... -
Better Dyeing Through Chemistry & Small Molecule Fluorophores
TypeBlog Post...motif attached to a fluorophore (Figure 1b). Engineered ligases (e.g., lipoic acid ligase, biotin ligase... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post...beginning of the third chromosome, ten base pairs in length. An emerging family of sequencing techniques function... -
Plasmids 101: Colony PCR
TypeBlog Post...Promega Overview for Screening for Recombinants New England Biolabs Overview of Colony PCR Additional Resources... -
A Career in Grants: How To Become a Grant Professional
TypeBlog Post...Becoming a grant professional is rewarding and challenging! Jo Miller, GPC, CSMS is the founder and principal... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog Post...the transfer plasmid. The deletion prevents full-length viral RNA from being produced in target cells and... -
Negotiating Work and Life: How to Find the Joy
TypeBlog Post...your plan to live a good life may be the most challenging assay you will ever have the opportunity to develop... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog Post...blocking initiation or splicing. (RNAi, in contrast, engages existing gene regulatory mechanisms to block activity... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog Post...uses a nuclease-dead variant of Cas9 (dCas9) and engineered combinations of transcriptional activators to... -
"Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science
TypeBlog Post...describing the labeled regions, as well as the strength and density of labeling in each. This allows users... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog Post...countries means that our shipping team is always engaged in some form of problem-solving. When universal... -
Hot Plasmids: Fall 2024
TypeBlog Post...common microscopy methods. Plus, the luminescence wavelength (460 nm) is shorter than many popular fluorescence... -
To Codon Optimize or Not: That is the Question
TypeBlog Post...optimization? Codon optimization is an approach in gene engineering to improve gene expression by changing synonymous...