We narrowed to 808 results for: eng

1. Experimenting with New Careers while in Grad School

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   Blog Post

   ...realized I wanted a career in science outreach: engaging the public to promote an awareness and understanding...

2. Genome-wide Screening Using CRISPR

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   ...multiple replicates and several cell types can strengthen your results. Enrichment or depletion of multiple...

3. Light Sheet Fluorescence Microscopy

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   ...C.D. Hojer, J.G. Egen, F. Hellal, F. Bradke, M. Sheng, H.-U. Dodt. (2012). Nature Protocols, 7(11), 1983...

4. Tips for Using BLAST to Verify Plasmids

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   ...included in the search to maximize the alignment length. While this recommendation is not infallible, we...

5. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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   ...also theoretically possible, but likely more challenging, with lentiviral expression. For more detail ...

6. Getting to Know Expansion Microscopy

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   ...traditional full IgG secondaries, can improve signal strength (Sherry and Stiles, 2022). Fourth, signal continuity...

7. Simplify Cloning with in vivo Assembly

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   ...temperature (Tm) of around 60 °C for all primers. The length of the homologous region should be around 20 bp...

8. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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   ...motif attached to a fluorophore (Figure 1b). Engineered ligases (e.g., lipoic acid ligase, biotin ligase...

9. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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   ...beginning of the third chromosome, ten base pairs in length. An emerging family of sequencing techniques function...

10. Plasmids 101: Colony PCR

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    ...Promega Overview for Screening for Recombinants New England Biolabs Overview of Colony PCR Additional Resources...

11. A Career in Grants: How To Become a Grant Professional

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    ...Becoming a grant professional is rewarding and challenging!  Jo Miller, GPC, CSMS is the founder and principal...

12. Your Lentiviral Plasmid FAQs Answered

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    ...the transfer plasmid. The deletion prevents full-length viral RNA from being produced in target cells and...

13. Negotiating Work and Life: How to Find the Joy

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    ...your plan to live a good life may be the most challenging assay you will ever have the opportunity to develop...

14. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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    ...blocking initiation or splicing. (RNAi, in contrast, engages existing gene regulatory mechanisms to block activity...

15. New Tools Enable CRISPRa for Neuroscience Applications

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    ...uses a nuclease-dead variant of Cas9 (dCas9) and engineered combinations of transcriptional activators to...

16. Transferable Skills Guide: External Collaborations

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    ...relationships. Personally, I find this to be the most challenging and time-intensive part of relationship building...

17. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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    ...Induced Pluripotent Stem Cells (iPSCs): Benefits, Challenges and the Road Ahead." Journal of Alzheimer's disease...

18. AAV Titers: Where Do They Come From And What Do They Mean?

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    ...28546538. PubMed Central PMCID: PMC5445070. 6. Wang, Feng, et al. "A reliable and feasible qPCR strategy for...

19. Transferable Skills Guide: Career Planning Resources

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    ...development (or lack thereof) One of the biggest challenges facing students today is that many graduate and...

20. Fluorescent Proteins 101: Aptamer Fluorophores

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    .... PubMed Central PMCID: PMC4800036. 12. Wang, Rongsheng E., et al. “Aptamer-Based Fluorescent Biosensors...