We narrowed to 802 results for: eng

1. Getting to Know Expansion Microscopy

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   ...traditional full IgG secondaries, can improve signal strength (Sherry and Stiles, 2022). Fourth, signal continuity...

2. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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   ...also theoretically possible, but likely more challenging, with lentiviral expression. For more detail ...

3. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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   ...motif attached to a fluorophore (Figure 1b). Engineered ligases (e.g., lipoic acid ligase, biotin ligase...

4. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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   ...beginning of the third chromosome, ten base pairs in length. An emerging family of sequencing techniques function...

5. Plasmids 101: Colony PCR

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   ...Promega Overview for Screening for Recombinants New England Biolabs Overview of Colony PCR Additional Resources...

6. A Career in Grants: How To Become a Grant Professional

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   ...Becoming a grant professional is rewarding and challenging!  Jo Miller, GPC, CSMS is the founder and principal...

7. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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   ...that provides a community for biologists and bioengineers to share their educational materials, protocols...

8. Your Lentiviral Plasmid FAQs Answered

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   ...the transfer plasmid. The deletion prevents full-length viral RNA from being produced in target cells and...

9. Negotiating Work and Life: How to Find the Joy

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   ...your plan to live a good life may be the most challenging assay you will ever have the opportunity to develop...

10. Plasmids 101: Codon usage bias

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    ...‐gene study reveals a high success rate of full‐length human proteins expressed in Escherichia coli." ...

11. Plasmids 101: Plasmid Incompatibility

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    ....0172913 Pubmed PMID: 28245276 Liu X, Wang D, Wang H, Feng E, Zhu L, Wang H (2012) Curing of Plasmid pXO1 from...

12. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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    ...Shawnna Buttery, Kat Milligan-Myhre, and Pragati Chengappa.  Financial and moral supporters Denise Akob, ...

13. To Codon Optimize or Not: That is the Question

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    ...optimization? Codon optimization is an approach in gene engineering to improve gene expression by changing synonymous...

14. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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    ...blocking initiation or splicing. (RNAi, in contrast, engages existing gene regulatory mechanisms to block activity...

15. New Tools Enable CRISPRa for Neuroscience Applications

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    ...uses a nuclease-dead variant of Cas9 (dCas9) and engineered combinations of transcriptional activators to...

16. Twenty Years of Sharing: Expanding Accessibility

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    ...countries means that our shipping team is always engaged in some form of problem-solving. When universal...

17. Hot Plasmids: Fall 2024

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    ...common microscopy methods. Plus, the luminescence wavelength (460 nm) is shorter than many popular fluorescence...

18. Hot Plasmids Spring 2024

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    ...This is similar to the proposed mechanism for engineered pegRNAs (epegRNAs), which add a structural motif...

19. Tips for Writing a Good Cover Letter

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    Blog Post

    ...skills and experiences fit the job requirements, strengthened an application. Even so, there were examples...

20. Plasmids 101: Broad Host Range Plasmids

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    ...across gram negative hosts. Biotechnology and Bioengineering, 106(2), 326–332. https://doi.org/10.1002/bit...